E-325

Material Data Sheet

UCH-L3

Price: $110.00
Catalog #: E-325

UCH-L3

Price: 
$110
Quantity: 
25 μg
Data Sheet: 
application/pdf icon325_hUchL3.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 7.6, 1 mM DTT. Actual concentration will vary with specific Lot #.

MW: 
26 kDa
Purity: 
> 95% by SDS-PAGE

Also known as: Ubiquitin C-terminal Hydrolase L3 (UCH-L3)

Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters from the C-terminus of ubiquitin. UCH-L3 is a member of the low molecular weight UCHs that processes ubiquitin precursors and ubiquitinated proteins to generate monomeric ubiquitin. This enzyme is also able to cleave at the C-terminus of NEDD8. UCH-L3, like UCH-L3 may have roles in the maintenance of neurons in the gracile tract, nucleus tractus solitaris and area postrema.

Use & Storage

Use: 
Typical enzyme concentration to support hydrolysis of substrates in vitro is 0.05-5 nM depending on conditions and substrate. Pre-incubation for 15 minutes with 10 mM DTT is recommended to achieve maximum activity.
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Day I.N., et al. (1990) Biochem J. 268:521-524
Dang L.C. et al. (1998) Biochem. 37:1868-1879
Johnston S.C., et al. (1997) EMBO.J. 16:3787-3796
Johnston S.C., et al. (1999) EMBO.J. 18:3877-3887
Kurihara L.J., et al. (2000) Mol. Cell. Biol. 20:2498-2504
Leroy E., et al. (1998) DNA.Res. 5:397-400
Mayer A.N. and Wilkinson K.D. (1989) Biochem. 28:166-172
Pickart C.M., et al. (1985) J.Biol.Chem. 260:7903-7910
Wada H., et al. (1998) Biophys. Biochem. Res. Comm. 351:688-692
Wilkinson K.D., et al. (1989) Science. 246:670-673
Wilkinson K.D., et al. (1999) J. Mol. Biol. 291:1067-1077