Material Data Sheet
Also known as: Ubiquitin C-terminal Hydrolase L3 (UCH-L3)
Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters from the C-terminus of ubiquitin. UCH-L3 is a member of the low molecular weight UCHs that processes ubiquitin precursors and ubiquitinated proteins to generate monomeric ubiquitin. This enzyme is also able to cleave at the C-terminus of NEDD8. UCH-L3, like UCH-L3 may have roles in the maintenance of neurons in the gracile tract, nucleus tractus solitaris and area postrema.
Stock:X mg/ml (X μM) in 50 mM HEPES pH 7.6, 1 mM DTT. Actual concentration will vary with specific Lot #.
Purity:> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support hydrolysis of substrates in vitro is 0.05-5 nM depending on conditions and substrate. Pre-incubation for 15 minutes with 10 mM DTT is recommended to achieve maximum activity.
Store at -80°C. Avoid multiple freeze/thaw cycles.
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