E-320

Material Data Sheet

Isopeptidase T/USP5

Price: $110.00
Catalog #: E-320

Isopeptidase T/USP5

Price: 
$110 $175
Quantity: 
10 μg 25 μg
Data Sheet: 
application/pdf icon320_rIsoT.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES, 150 mM NaCl, 1mM DTT, pH 8.0 Actual concentration will vary with specific Lot #.

MW: 
97 kDa
Purity: 
> 95% by SDS-PAGE

Also known as: IsoT

Isopeptidase T is a member of the high molecular weight ubiquitin C-terminal hydrolases (UCHs) that are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters from the C-terminus of ubiquitin. This enzyme primarily disassembles free polyubiquitin chains linked through isopeptide bonds. This activity has a critical role in the recycling of Ub and in the regulation of the activity of the 26S proteasome.

Use & Storage

Use: 
Typical enzyme concentration to support hydrolysis of substrates in vitro ranges from 10-100 nM depending on conditions and substrate. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dang L.C., et al. (1998) Biochemistry 37:1868-18679
Falquet L., et al. (1995) FEBs Lett. 376:233-237
Haldari T., et al. (1992) J.Biol. Chem. 267:719-727
Lacombe T., et al. (2002) FEBS Lett. 531:469-474
Lam Y.A., et al. (1997) Nature 385:737-740
Wilkinson K.D., et al. (1995) Biochemistry 34:14535-14546
Stein R.L., et al. (1995) Biochemistry 34:12616-12623
Wing S.S (2003) Int. J. Biochem. Cell. Biol. 35:590-605