E-319

Material Data Sheet

His6-UFM1 Activating Enzyme (UBA5)

Price: $250.00
Catalog #: E-319

His6-UFM1 Activating Enzyme (UBA5)

Price: 
$250
Quantity: 
25 μg
Data Sheet: 
application/pdf icon319_His Ufm1 E1_Uba5.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH8.0. Concentration varies with lot number.

MW: 
48 kDa
Purity: 
> 95% by SDS-PAGE

Also known as: UBA5

His6-UFM1 Activating Enzyme is the E1-like enzyme responsible for the activation of the ubiquitin-like modifier, Ufm1 (UL-500). The enzyme contains a conserved ThiF domain with a nearby cysteine catalytic residue (Cys250)and ATP-binding motif GXGXXG. This protein is highly conserved in humans and other higher eukaryotes, but not yeast.The enzyme utilizes ATP to adenylate the C-terminal glycine residue of Ufm1, forming a high-energy thiolester bond with the active site cysteine residue and the release of AMP. The second step is the trans-esterification reaction whereby Ufm1 is transferred to the E2-like protein Ufc1 (E2-675). This protein has an N-terminal His6-tag.

Use & Storage

Use: 
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dou, T. et al. (2005) Molec. Biol. Rep. 32: 265-271.

Komatsu, M et al. (2004) EMBO J. 23: 1977-1986.