E-318

Material Data Sheet

His6-ATG7 isoform 1

Price: $299.00
Catalog #: E-318

His6-ATG7 isoform 1

Price: 
$299
Quantity: 
50 µg
Data Sheet: 

ATG7 is a 78 kDa cytosolic ubiquitin­E1­like enzyme that plays a key role in the autophagy pathway of intracellular bulk degradation.  It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8 proteins including GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A, and the subsequent formation of autophagosomes.  ATG7 is required for mitochondrial removal during erythropoiesis (“mitophagy”) and for the maintenance of axonal homeostasis. Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C­terminus, a region that is required for ATG8 lipidation. Human ATG7 shares 93% aa sequence identitiy with mouse and rat ATG7.  This recombinant proteins has an N-terminal 6-His tag.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.0, 150 mM NaCl, 2 mM TCEP, 10% glycerol
MW: 
80 kDa (monomer size)
Purity: 
> 90% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 

Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Ichimura Y., et al. (2000) Nature 408: 488-492

Komatsue M., et al. (2001) J. Biol. Chem. 276: 9846-9854

Mizushima N., et al. (1998) J. Biol. Chem. 273: 33889-33892

Ohsumi Y., et al. (2001) Nat. Rev.  2: 211-216

Reggiori F. and Klionsky D.J. (2002)  Euk. Cell.  1:11-21

Tanida I., et al. (2001) J. Biol. Chem. 276: 1701-1706

Tanida I., et al. (2002) J. Biol. Chem. 277: 13739-13744

Yu L., et al. (2004) Science 302: 1500-1502

Yuan W., et al. (1999) Mol. Cell. Biol. 10: 1353-1366