Material Data Sheet

His6-ATG7 isoform 1

Price: $299.00
Catalog #: E-318

His6-ATG7 isoform 1

50 µg
Data Sheet: 

ATG7 is a 78 kDa cytosolic ubiquitin­E1­like enzyme that plays a key role in the autophagy pathway of intracellular bulk degradation.  It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8 proteins including GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A, and the subsequent formation of autophagosomes.  ATG7 is required for mitochondrial removal during erythropoiesis (“mitophagy”) and for the maintenance of axonal homeostasis. Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C­terminus, a region that is required for ATG8 lipidation. Human ATG7 shares 93% aa sequence identitiy with mouse and rat ATG7.  This recombinant proteins has an N-terminal 6-His tag.

Product Information

X mg/ml (X µM) in 50 mM HEPES pH 7.0, 150 mM NaCl, 2 mM TCEP, 10% glycerol
80 kDa (monomer size)
> 90% by SDS-PAGE

Use & Storage


Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.


Store at –80°C. Avoid multiple freeze/thaw cycles.



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