E-315

Material Data Sheet

SUMO E1 (SAE1/UBA2)

Price: $225.00
Catalog #: E-315

SUMO E1 (SAE1/UBA2)

Price: 
$225.00
Quantity: 
25 µg

Conjugation of the ubiquitin-like modifier SUMO (Sentrin) requires the activities of the heterodimeric E1 (SAE1/SAE2) and the UBE2I (Ubc9) E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of all SUMO proteins, forming a high- energy thiolester bond with the cysteine residue of SAE2 and the concomitant release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO is transferred to Cys93 of UBE2I.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8, 10% (v/v) Glycerol, 200 mM NaCl, 1 mM DTT
MW: 
110 kDa
Purity: 
> 90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 

Store at –80°C. Avoid multiple freeze/thaw cycles.