E-315

Material Data Sheet

SUMO E1 (SAE1/UBA2)

Price: $200.00
Catalog #: E-315

SUMO E1 (SAE1/UBA2)

Price: 
$200
Quantity: 
25 μg
Data Sheet: 
application/pdf icon315_hsumoE1.pdf
Stock: 

X mg/ml (X μM) in 50 mM Hepes pH 8, 200 mM NaCl, 1 mM DTT, 10% glycerol. Actual concentration will vary with specific lot number.

MW: 
110 kDa
Purity: 
> 90% by SDS-PAGE

Also known as: SUMO Activating Enzyme (SAE1/SAE2), or UBA2

Conjugation of the ubiquitin-like modifier SUMO (Sentrin) requires the activities of the heterodimeric E1 (SAE1/SAE2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of all SUMO proteins, forming a high- energy thiolester bond with the cysteine residue of SAE2 and the concomitant release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO is transferred to Cys93 of UbcH9.

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Azume Y., et al. (2001) FASEB. J. 15:1825-1827

Desterro JMP.,  et al. (1999) J. Biol. Chem. 274:10618-10624

Okuma T.,  et al (1999) Biochem Biophys Res Commun. 254:693698

Tatham M.H.,  et al. (2001) J. Biol. Chem. 276:35368-35374