E-312

Material Data Sheet

GST-NEDD8 E1 (APPBP1/UBA3)

Price: $295.00
Catalog #: E-312

GST-NEDD8 E1 (APPBP1/UBA3)

Price: 
$295
Quantity: 
50 µg

Also know as: GST-NEDD8 Activating Enzyme (APPBP1/Uba3)

The ATP-coupled activation of NEDD8 that is required for subsequent charging of the NEDD8-specific E2 UbcH12 is catalyzed by heterodimeric APPBP1-Uba3 in humans. The enzyme catalyzes the activation of the C-terminal carboxyl group of NEDD8 by forming a high-energy thioester bond in an ATP-dependent manner.    Uba3 shows 43% homology to the C-terminal half of the ubiquitin activating E1 enzyme Uba1. The Uba3- catalyzed activation of NEDD8 exhibits an absolute requirement for APPBP1 which has high homology to the N-terminal half of Uba1.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl. Actual concentration will vary with specific lot number.
MW: 
135 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Lake M.W., et al. (2001) Nature. 414:325-328

Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95

Walden H., et al. (2003) Nature. 422:330-334