E-311

Material Data Sheet

SUMO E1 (SAE1/UBA2)

Price: $250.00
Catalog #: E-311

SUMO E1 (SAE1/UBA2)

Price: 
250
Quantity: 
25 µg
Data Sheet: 
application/pdf icone-311-sumo-e1-sae1uba2.pdf

Also known as: SUMO Activating Enzyme (Aos1/Uba2)

Conjugation of the ubiquitin-like modifier SUMO (Sentrin) requires the activities of the heterodimeric E1 (Aos1/Uba2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of SUMO-1 (also SUMO-2 and SUMO- 3), forming a high-energy thiolester bond with the cysteine residue of Uba2 and the release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO-1 is transferred to Cys93 of UbcH9.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH8.0, 150 mM NaCl. Actual concentration will vary with specific Lot #.
MW: 
114 kDa
Purity: 
> 90% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dohmen R.J., et al. (1995) J. Biol. Chem. 270:18099-18109

Gong L., et al. (1999) FEBS Lett. 448:185-189

Johnson E.S., et al. (1997) EMBO J. 16:5509-5519

Okuma T., et al. (1999) Biochem Biophys Res Commun. 254:693698

Tatham M.H., et al. (2001) J. Biol. Chem. 276:35368-35374