E-310

Material Data Sheet

GST-SUMO E1 (SAE1/UBA2)

Price: $275.00
Catalog #: E-310

GST-SUMO E1 (SAE1/UBA2)

Price: 
$275
Quantity: 
50 μg
Data Sheet: 
application/pdf icon310_gstSumoE1.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH8.0, 150 mM NaCl. Actual concentration will vary with specific Lot #.

MW: 
140 kDa
Purity: 
> 90% by SDS-PAGE

Conjugation of the ubiquitin-like modifier SUMO requires the activities of the heterodimeric E1 (Aos1/Uba2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of SUMO-1 (also SUMO-2 and SUMO-3), forming a high-energy thiolester bond with the cysteine residue of Uba2 and the release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO-1 is transferred to Cys93 of UbcH9.

Use & Storage

Use: 
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dohmen R.J., et al. (1995) J. Biol. Chem. 270:18099-18109

Gong L., et al. (1999) FEBS Lett. 448:185-189

Johnson E.S., et al. (1997) EMBO J. 16:5509-5519

Okuma T., et al. (1999) Biochem Biophys Res Commun. 254:693698

Tatham M.H., et al. (2001) J. Biol. Chem. 276:35368-35374