Material Data Sheet
Also known as: UBA7
This E1 is responsible for the first step in ISG15-protein isopeptide bond formation. This enzyme catalyzes the activation of the C-terminal carboxyl group of ISG15 by forming a high-energy thioester bond in an ATP-dependent manner. The activated ISG15 is then transferred to UbcH8 and subsequently to lysine groups of target proteins via this thiolester conjugation cascade. The E1 enzyme is a critical component for the initiation of any in vitro conjugation reactions.
Stock:X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual concentration will vary with specific Lot #.
Purity:> 90% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Store at –80°C. Avoid multiple freeze/thaw cycles.
Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95
Yuan W., et al. (2001) EMBO. J. 20: 362-371