E-309

Material Data Sheet

ISG15 E1/UBE1L

Price: $275.00
Catalog #: E-309

ISG15 E1/UBE1L

Price: 
$275
Quantity: 
25 μg
Data Sheet: 

Also known as: UBA7

This E1 is responsible for the first step in ISG15-protein isopeptide bond formation. This enzyme catalyzes the activation of the C-terminal carboxyl group of ISG15 by forming a high-energy thioester bond in an ATP-dependent manner. The activated ISG15 is then transferred to UbcH8 and subsequently to lysine groups of target proteins via this thiolester conjugation cascade. The E1 enzyme is a critical component for the initiation of any in vitro conjugation reactions.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual concentration will vary with specific Lot #.
MW: 
112 kDa
Purity: 
> 90% by SDS-PAGE

Use & Storage

Use: 
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95

Yuan W., et al. (2001) EMBO. J. 20: 362-371