E-306

Material Data Sheet

GST-Ubiquitin Activating Enzyme (UBE1)

Catalog #: E-306

GST-Ubiquitin Activating Enzyme (UBE1)

Datasheet: 
application/pdf icon306_hGSTE1.pdf
Product Source: 

human recombinant

This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its C-terminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions.

Product Information
Quantity: 

50 μg

Price: 

$195

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0. Actual concentration varies with lot number

MW: 

136 kDa

Purity: 

> 95% by SDS-PAGE

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 

Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature
References: 

Ciechanover A., et al. (1982) J. Biol. Chem. 257:2537-2542
Haas A.L. and Rose I.A. (1982) J. Biol. Chem. 257:1032910337
Haas A.L., et al. (1982) J. Biol. Chem. 257:2543-2548
Handley P.M., et al. (1991) Proc. Natl. Acad. Sci. 88:258-262
Jonnalagadda S., et al. (1988) J. Biol. Chem. 263:5016-5019
Pickart C.M., et al. (1994) J. Biol. Chem. 269:7115-7123
Salvat C., et al. (2000) Eur. J. Biochem. 267:3712-3722
Stephen A.G., et al (1996) J. Biol. Chem. 271:15608-15614
Wilkinson K.D., et al. (1990) Biochem. 29:7373-7380