Material Data Sheet

His6-Ubiquitin E1 Enzyme (UBE1)

Price: $195.00
Catalog #: E-304

His6-Ubiquitin E1 Enzyme (UBE1)

50 µg
Data Sheet: 

This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its C-terminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions. Protein has a tag at the N-terminus.

Product Information

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 1 mM TCEP
121 kDa
> 95% by SDS-PAGE

Use & Storage


Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.


Store at –80°C. Avoid multiple freeze/thaw cycles.



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