E-301

Material Data Sheet

Ubiquitin Activating Enzyme (UBE1)

Price: $275.00
Catalog #: E-301

Ubiquitin Activating Enzyme (UBE1)

Price: 
$275
Quantity: 
50 μg
Data Sheet: 
application/pdf icon301_yE1.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH8.0. Concentration varies with Lot #.

MW: 
110 kDa
FW: 
95% by SDS-PAGE

This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its C-terminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in E1, yielding an ubiquitin-E1 thiolester and free AMP. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. E1 is a critical component for the initiation of any in vitro conjugation reactions.

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Ciechanover A., et al. (1982) J. Biol. Chem. 257:2537-2542

Ghaboosi N. and Deshaies R.J. (2007) Mol.Biol.Cell. 181:1953-1963

Haas A.L. and Rose I.A. (1982) J. Biol. Chem. 257:1032910337

Haas A.L., et al. (1982) J. Biol. Chem. 257:2543-2548

Handley P.M., et al. (1991) Proc. Natl. Acad. Sci. 88:258-262

Hoefer M and Cook J.C. (1991) FEBS. Lett. 289:54-59

Jonnalagadda S., et al. (1988) J. Biol. Chem. 263:5016-5019

MacGrath J.P. et al. (1991) EMBO J. 10:227-236

Pickart C.M., et al. (1994) J. Biol. Chem. 269:7115-7123

Salvat C., et al. (2000) Eur. J. Biochem. 267:3712-3722

Stephen A.G., et al. (1996) J. Biol. Chem. 271:15608-15614

Wilkinson K.D., et al. (1990) Biochem. 29:7373-7380