Material Data Sheet

5X Loading Buffer

Price: $35.00
Catalog #: B-125

5X Loading Buffer

1 mL
Data Sheet: 

Loading buffer for separation and visualization proteins with SDS-PAGE and Western Blot analysis. Sample can be prepared under reducing conditions with the addition of a reducing agent such as β-mercaptoethanol or DTT.

Use & Storage


Dilute to 1X final when adding to sample.  For reducing conditions, add 5% (v/v) β-mercaptoethanol or 10-20 mM DTT, final, to 1X loading buffer and sample.  Heat sample/loading buffer to desired temperature for 5 minutes prior to loading on SDS-PAGE gel.  Do not store buffer with reducing agent; add fresh reducing agent immediately prior to each use.


Store at room temperature.