B-100

Material Data Sheet

50X E1 Stop Buffer

Price: $20.00
Catalog #: B-100

50X E1 Stop Buffer

Price: 
$20
Quantity: 
1 ml
Data Sheet: 
application/pdf iconB-100_50X E1 Stop Buffer_.pdf
Stock: 

1ml of 0.5M EDTA 

This buffer contains the optimal formulation to stop thiolester/conjugation reactions dependent on E1 activating enzyme activities which are Mg-ATP dependent. Ethylenediamine tetracetic acid (EDTA) is a metal chelator that quenches activity of these Mg-ATP dependent enzymes when added to an assay in excess.

Use & Storage

Use: 

Dilute to 1X in reaction to stop enzyme activity for final concentration of 10mM in assay.

Storage: 
Store at -20°C. Avoid multiple freeze/thaw cycles.