AP-120

Material Data Sheet

E6, Human Papillomavirus type 16

Price: $125.00
Catalog #: AP-120

E6, Human Papillomavirus type 16

Price: 
$125
Quantity: 
25 μg

E6 (Early protein 6) is a viral protein produced in cells infected with the Human Papillomavirus. E6 forms a complex with the host cell ubiquitin ligase E6AP (E6-Associated-Protein) generating a ligase activity that polyubiquitinates tumor suppressors p53 and p73 and targets them to the 26S proteasome for degradation. As a result DNA damage and chromosomal instabilities increase, often leading to cell proliferation and cancer. The E6/E6AP complex also targets other substrates for ubiquitination, such as TERT, BAK1, FADD, and pro-CASP8—none of which appear to be substrates for E6AP in the absence of E6. This protein is untagged, and contains six cysteine-to-serine substitutions at positions 23, 58, 87, 104, 118, and 147 (HPV type 16 sequence numbering).

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.0, 400 mM NaCl, 2 mM DTT
MW: 
19 kDa
Purity: 
> 90% by SDS-PAGE

Use & Storage

Use: 

Typical concentration to support E6AP-mediated p53 ubiquitination in vitro is 0.1-2 μM depending on experimental conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.