Material Data Sheet

Ubiquilin 1 Tandem UBA (TUBE2) Agarose

Price: $169.00
Catalog #: AM-130

Ubiquilin 1 Tandem UBA (TUBE2) Agarose

250 µL


Ubiquilin-1 contains an N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. It associates with proteasomes and ubiquitin ligases, and is thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. Ubiquilin-1 has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display increased affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). TUBEs also display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. This affinity resin can be used for the enrichment, isolation and identification of K48-linked (preferentially) or K63-linked poly-Ub chains or ubiquitinated substrates that contain these linkages.

Product Information

0.25 ml of TUBE2 Agarose is supplied in a 0.5 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl.

Use & Storage


Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

The agarose can be re-used for at least 2 applications if properly maintained. After use, clean resin with 5 ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C, 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.




Bertram, L., et al. (2005) New Eng. J. Med. 352: 884-894

Bird, T. D. (2008) New Eng. J. Med. 352: 862-864,

Ganguly, A., et al. (2008) Hum. Molec. Genet. 17: 293-302

Hanaoka, E., et al. (2000) J. Hum. Genet. 45: 188-191

Hjerpe, R., et al. (2009) EMBO Reports. 10: 1250-1258

Hurley, J., et al. (2006) Biochem. J. 399: 361-372

Kamboh, M. I., et al. (2006) Molec. Psychiat. 11: 273-279

Kleijnen, M. F., et al. (2000) Molec. Cell 6: 409-419

Ozaki, T., et al. (1997) DNA Cell Biol. 16: 985-991

Slifer, M. A., et al.(2005) New Eng. J. Med. 352: 2752-2753

Smemo, S.; Nowotny, P., et al. (2006) Ann. Neurol. 59: 21-26

Sweetser, D. A., et al. (2005) Genes Chromosomes Cancer 44: 279-291