AM-125

Material Data Sheet

HR23A Tandem UBA (TUBE1) Agarose

Price: $159.00
Catalog #: AM-125

HR23A Tandem UBA (TUBE1) Agarose

Price: 
$159
Quantity: 
250 µL

hHR23A has two UBA domains that can each bind ubiquitin in addition to an N-terminal UBL domain that binds S5a and S2, two components of the 26S proteasome. hHR23a recognizes ubiquitin through a predominately hydrophobic surface formed by residues within α1 and α3 of each of its UBA domains. Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display increased affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). TUBEs also display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. This affinity resin can be used for the enrichment, isolation and identification of K63-linked (preferentially) or K48-linked poly-Ub chains or ubiquitinated substrates that contain these linkages.

Product Information

Stock: 
  0.25 ml of TUBE1 agarose is supplied in a 0.5 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl.

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

Storage: 
The agarose can be re-used for at least 2 applications if properly maintained. After use, clean resin with 5 ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C, 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.

Literature

References: 

 

Hjerpe, R., et al. (2009) EMBO Reports. 10: 1250-1258

Hurley, J., et al. (2006) Biochem. J. 399: 361-372

Masutani, C, et al. (1994) EMBO J. 13: 1831-1843

Van der Spek, P.J., et al. (1994) Genomics. 23: 651-658

Van der Spek, P.J., et al. (1996) Genomics. 31: 20-27

Wang, G., et al. (2000) Hum. Molec. Genet. 9: 1795-1803