AM-120

Material Data Sheet

RAP80 UIM Domains Agarose

Price: $215.00
Catalog #: AM-120

RAP80 UIM Domains Agarose

Price: 
$215
Quantity: 
250 µL
Data Sheet: 

Rap80 (Receptor Associated Protein 80) interacts with BRCA1, a ubiquitin E3-ligase which functions in conjugation with the BARD1 deubiquitinating enzyme. BRCA1 is recruited to DNA damage sites by poly-ubiquitin chains through Rap80 which contains 2 tandem ubiquitin-interacting motifs (UIMs). Rap80 constitutes a protein complex with ABRA1 which interacts with the BRCT domain of BRCA1. Upon DNA damage the Rap80-ABRA1 complex targets the BRCA1-BARD1 complex to K6- and K63-linked poly-Ub chains at these foci. The UIM domains of Rap80 have been shown to have preferential binding to K-6 and K63-linked Ub chains and binds to K48-chain with a much lower efficiency. It is not known if Rap80 UIMs interact with Ub chains linked via K11, K27 or K33. Rap80 has a low affinity for mono-, di- and tri-Ub but binds efficiently to tetra (or greater) ubiquitin chains. This affinity resin can be used for the enrichment, isolation and identification of ubiquitinated proteins that are non-K48-linked.

Product Information

Stock: 
0.25 ml RAP80 UIM domains agarose is supplied in a 0.5 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl, 1 mM NaN3.

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

Storage: 
The agarose can be re-used for at least 2 applications if properly maintained. After use, clean resin with 5 ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C, 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.

Literature

References: 

Buchberger A. (2002) Tren. Cell. Biol. 12 :216-221
Hurley J.H., et al. (2006) Biochem. J. 399 :361-372
Kim H., et al. (2007) Science. 316:1202-1205
Kim H., et al. (2008) Mol. Cells. 25:457-61
Sobhian B., et al. (2007) Science. 316:1198-1202
Wang B., (2007) Science. 316:1194-1198
Wu W., et al. (2008) Cell. Div. 31-10
Yan J., et al. (2007) Canc. Res. 15:6647-6656