Material Data Sheet

Ataxin UIM Domains Agarose

Price: $215.00
Catalog #: AM-115

Ataxin UIM Domains Agarose

250 µL
Data Sheet: 

Ataxin-3 protein belongs to a novel group of cysteine proteases similar to USP-type ubiquitin proteases and has deubiquitinating activity in vitro. The full-length protein contains an catalytic N-terminal Josephin domain, three ubiquitin interacting motifs (UIMs), and a variable C-terminus with a polyglutamine stretch. Ataxin-3 has deconjugating activity and functions as a mixed linkage, chain editing enzyme with preferential cleavage of K63 linkages in mixed chains. Ataxin3 also bind both K48-linked and K63-linked poly-Ub chains via its UIM domains and preferentially interacts with four or more ubiquitin units. This affinity resin can be used for the enrichment, isolation and identification of K48-linked (preferentially) or K63-linked poly-Ub chains or ubiquitinated substrates that contain these linkages.

Product Information

0.25 ml of Ataxin UIM domains agarose is supplied in 0.5 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl.

Use & Storage


Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions. 


The agarose can be re-used for at least 2 applications if properly maintained. After use, clean resin with 5 ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C, 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.



Albrecht., et al. (2004) Eur. J. Biochem. 271:3155-3170
Buchberger A. (2002) Tren. Cell. Biol. 12 :216-221
Burnett B., et al. (2003) Hum. Mol Genet. 12 :3195-3205
Hurley J.H., et al. (2006) Biochem. J. 399 :361-372
Mao Y., et al. (2005)Proc. Natl. Acad. Sci. 102 :12700-12705
Shoesmith S.J., et al. (2005) J. Biol. Chem. 280 :32026-32034