AM-115

Material Data Sheet

Ataxin UIM Domains Agarose

Price: $215.00
Catalog #: AM-115

Ataxin UIM Domains Agarose

Price: 
$215
Quantity: 
250 µL
Data Sheet: 

Ataxin-3 protein belongs to a novel group of cysteine proteases similar to USP-type ubiquitin proteases and has deubiquitinating activity in vitro. The full-length protein contains an catalytic N-terminal Josephin domain, three ubiquitin interacting motifs (UIMs), and a variable C-terminus with a polyglutamine stretch. Ataxin-3 has deconjugating activity and functions as a mixed linkage, chain editing enzyme with preferential cleavage of K63 linkages in mixed chains. Ataxin3 also bind both K48-linked and K63-linked poly-Ub chains via its UIM domains and preferentially interacts with four or more ubiquitin units. This affinity resin can be used for the enrichment, isolation and identification of K48-linked (preferentially) or K63-linked poly-Ub chains or ubiquitinated substrates that contain these linkages.

Product Information

Stock: 
0.25 ml of Ataxin UIM domains agarose is supplied in 0.5 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl.

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions. 

Storage: 

The agarose can be re-used for at least 2 applications if properly maintained. After use, clean resin with 5 ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C, 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.

Literature

References: 

Albrecht., et al. (2004) Eur. J. Biochem. 271:3155-3170
Buchberger A. (2002) Tren. Cell. Biol. 12 :216-221
Burnett B., et al. (2003) Hum. Mol Genet. 12 :3195-3205
Hurley J.H., et al. (2006) Biochem. J. 399 :361-372
Mao Y., et al. (2005)Proc. Natl. Acad. Sci. 102 :12700-12705
Shoesmith S.J., et al. (2005) J. Biol. Chem. 280 :32026-32034