AM-100

Material Data Sheet

S5a/Angiocidin Agarose

Price: $256.00
Catalog #: AM-100

S5a/Angiocidin Agarose

Price: 
$256
Quantity: 
0.5 ml
Data Sheet: 
application/pdf iconAM-100_S5a agarose.pdf
Stock: 

0.5 ml S5a/Angiocidin agarose is supplied in 1 ml total volume of 50 mM

Hepes pH 7.5, 250 mM NaCl, 1 mM NaN3.

S5a (Rpn10) is a component of the regulatory complex (19S) of the 26S proteasome. By binding to and recognizing poly-ubiquitinated proteins, it functions as a receptor for proteins destined for proteolytic degradation. The protein recognizes ubiquitin chains and conjugates via two ubiquitin-interacting motif (UIM) domains. S5a has a low affinity for mono-, di- and tri-Ub but binds efficiently to tetrameric ubiquitin and has a preference for longer Ub polymers. This affinity resin can be used for the enrichment, isolation and identification of ubiquitinated proteins, 26S substrates or proteins that contain ubiquitin-like domains.

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions. Some S5a may leach off the resin with each use.

Storage: 
The agarose can be re-used for at least 2 applications if properly maintained. After use, clean resin with 5 ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.

Literature

References: 

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Haririnia A., et al. (2007) J.Mol Biol. 368:753-766
Kang Y., et al. (2007) J. Mol Biol. 369:168-176.
Layfield R., et al. (2001) Proteomics 1:773-777
Mayor T., et al. (2005) Mol. Cell. Proteomics. 4:741-751.
Mueller T.D. and Feignon J. (2003) EMBO J. 22:4634-4645
Saeki Y., et al. (2002) Biochem Biophys Res Commun. 293:986-992
Ventadour S., et al. (2007) J. Biol. Chem. 282: 5302-5309
Wang,Q., et al. (2005) J. Mol. Biol. 348: 727-739