A-812

Material Data Sheet

NEDD8 Polyclonal Ab

Price: $150.00
Catalog #: A-812

NEDD8 Polyclonal Ab

Price: 
$150
Quantity: 
200 μl
Data Sheet: 
application/pdf iconA-812_antiNedd8.pdf
Stock: 

PBS pH 7.4, 20% glycerol.

Antigen: 
Full-length, highly purified human Nedd8 protein.
The ubiquitin-like protein NEDD8 is conjugated to targets by the NEDD8-specific E1 activating enzyme (APP-B1/UBA3), the Ubch12 E2 enzyme, and the ROC1/Rbx1 RING FINGER E3 ligase. NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family.
Storage: 
Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.
Specificity: 
Recommended dilution range for Western blot analysis is 1:100 to 1:1000. Antibody does not cross-react with ubiquitn, SUMO, or ISG15 proteins. Non-functional for immunoprecipitation.</p>

Literature

References: 

Gong L. et al. (1999) J. Biol. Chem. 274: 12036-12042
Hori T., et al. (1999) Oncogene. 18:6829-6834
Kamura T., et al. (1999) Genes. Dev. 13:2928-2933
Kumar S., et al. (1993) Biophys. Biochem. Res. Comm. 195:393-399
Morimoto M., et al. (2003) Biophys. Biochem. Res. Comm. 301:392-398
Wada H., et al. (1999) Biophys. Biochem. Res. Comm. 275:100-105
Whitby F.G., et al. (1998) J. Biol. Chem. 273: 34983-34991