A-718

Material Data Sheet

Human SUMO2/3, MAb

Price: $239.00
Catalog #: A-718
Human SUMO2/3, MAb
Price: 
$239
Quantity: 
100 µg
Data Sheet: 
Small Ubiquitin­like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post­translational modification process termed SUMOylation. Unlike ubiquitination, which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO1, SUMO2, SUMO3, and SUMO4 are all translated as propeptides, containing C-terminal prosegments following the diglycine motif that marks the end of the mature forms. Following prosegment cleavage, SUMO1, 2, and 3 may then be enzymatically attached to a lysine on a target protein. It is not clear whether SUMO4 is processed in a similar fashion.

Product Information

Stock: 
0.5 mg/mL in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide
Isotype: 
IgG2A. Clone # 852908
Western Blot Data: 
Antigen: 
Purified, recombinant human SUMO3. Accession Number P55854

Use & Storage

Use: 
Recommended concentration for Western Blot is 0.1 - 0.5 µg/ml.
Storage: 
Store at -20°C.
Specificity: 
This antibody detects endogenous, human SUMOylated proteins in Western blots. This antibody has equivalent reactivity to SUMO2 and SUMO3 in Western blots with recombinant SUMO proteins. It has less than 5% cross-reactivity with recombinant SUMO4 and no cross-reactivity with recombinant SUMO1.

Literature

References: 

Desterro J.M., et al. (1997) FEBs. Lett. 417: 297-300

Okama T., et al. (1999) Biochem. Biophys. Res. Comm. 254: 693-698

Seeler J-S. and Dejean A. (2003) Nat. Rev. 4: 690-699

Su H-L., et al. (2002) Gene 296: 65-73

Tatham M.H., et al. (2001) J. Biol. Chem. 276: 35368-35374

Yeh E.T.H., et al. (2000) Gene 248: 1-14