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Material Data Sheet
SUMO Polyclonal Ab
Price: $150.00
SUMO Polyclonal Ab
Price:
150
Quantity:
200 µL
Data Sheet:
The ubiquitin-like SUMO proteins are conjugated to a variety of proteins in the presence of UbcH9 and the Aos1/Uba2 (yeast) or SAE1/SAE2 (human) activating enzyme. Sumolyation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred
directly from UbcH9 to specific substrates. Sumolyated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation.
Product Information
Stock:
PBS pH 7.4
Antigen:
Full-length, highly purified human SUMO-1 protein. Use & Storage
Use:
Recommended dilution range for Western blot analysis is 1:500 to 1:1500. Antibody cross-reacts with SUMO1, SUMO2 and SUMO3 proteins, but not ubiquitin, Nedd8 or ISG15 proteins.
Storage:
Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.
Literature
References:
Desterro J.M., et al. (1997) FEBs. Lett. 417:297-300
Okama T., et al. (1999) Biochem. Biophys. Res. Comm. 254:693-698
Seeler J-S. and Dejean A. (2003) Nat. Rev. 4:690-699
Su H-L., et al. (2002) Gene 296:65-73
Tatham M.H., et al. (2001) J. Biol. Chem. 276:35368-35374
Yeh E.T.H., et al. (2000) Gene 248:1-14
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