A-655

Material Data Sheet

Anti-UbcH12

Catalog #: A-655

Anti-UbcH12

Datasheet: 
application/pdf iconA-655_antiH12.pdf
Product Source: 

rabbit polyclonal

also known as Ubc12,hUbc12, UBE2M

NEDDylation requires the coordinated activities of UbcH12 and the APP-BPI/Uba3 activating enzyme. This E2 enzyme mediates the covalent conjugation of the ubiquitin-like protein NEDD8 to a limited number of cellular proteins such as Cdc53/Cul1 and other members of the cullin family (which are structural subunits of the Skp1-based and elongin B/C-based families of ubiquitin protein ligases). The NEDD8 pathway is essential for cell viability in fission yeast, and in mammalian cells it is essential for cell cycle progression and morphogenesis.

Product Information
Quantity: 

200 μl

Price: 

$150

Stock: 

PBS pH 7.4, 20% glycerol.

Antigen: 

Full-length, highly purified human UbcH12 protein.

Supplied: 

Protein A affinity purified rabbit polyclonal antibody.

Use & Storage
Storage: 

Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.

Specificity: 

Recommended dilution range for Western blot analysis is 1:100 to 1:1000.

Literature
References: 

Amir R.E., et al. (2002) J. Biol. Chem. 277: 23253-23259
Gong L., et al. (1999) J. Biol. Chem. 274: 12036-12042
Hochstrasser M. (2000) Nat. Cell. Biol. 2: E153-E157
Osaka F., et al. (1998) Genes. Dev. 12:2263-2268
Podust V.N., et al. (2000) Proc. Natl. Acad. Sci. 97:4579-4584
Tateishi K., et al. (2001) J. Cell. Biol. 155:571-580
Wada H., et al. (2000) J. Biol. Chem. 275: 17008-17015