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Material Data Sheet
UbcH12/UBE2M Polyclonal Ab
Price: $150.00
UbcH12/UBE2M Polyclonal Ab
Price:
150
Quantity:
200 µL
Data Sheet:
NEDDylation requires the coordinated activities of Ubc12 and the APP-BPI/Uba3 activating
enzyme. This E2 enzyme mediates the covalent conjugation of the ubiquitin-like protein NEDD8
to a limited number of cellular proteins such as Cdc53/Cul1 and other members of the cullin
family (which are structural subunits of the Skp1-based and elongin B/C-based families of
ubiquitin protein ligases). The NEDD8 pathway is essential for cell viability in fission yeast, and
in mammalian cells it is essential for cell cycle progression and morphogenesis.
Product Information
Stock:
PBS pH 7.4, 20% glycerol.
Antigen:
Full-length, highly purified human UbcH12 protein.
Supplied:
Protein A affinity purified rabbit polyclonal antibody. Use & Storage
Use:
Recommended dilution range for Western blot analysis is 1:100 to 1:1000.
Storage:
Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.
Literature
References:
Amir R.E., et al. (2002) J. Biol. Chem. 277: 23253-23259
Gong L., et al. (1999) J. Biol. Chem. 274: 12036-12042
Hochstrasser M. (2000) Nat. Cell. Biol. 2: E153-E157
Osaka F., et al. (1998) Genes. Dev. 12:2263-2268
Podust V.N., et al. (2000) Proc. Natl. Acad. Sci. 97:4579-4584
Tateishi K., et al. (2001) J. Cell. Biol. 155:571-580
Wada H., et al. (2000) J. Biol. Chem. 275: 17008-17015
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