A-655

Material Data Sheet

UbcH12/UBE2M

Price: $159.00
Catalog #: A-655

UbcH12/UBE2M

Price: 
$159
Quantity: 
200 µL

NEDDylation requires the coordinated activities of Ubc12 and the APP-BPI/Uba3 activating enzyme. This E2 enzyme mediates the covalent conjugation of the ubiquitin-like protein NEDD8 to a limited number of cellular proteins such as Cdc53/Cul1 and other members of the cullin family (which are structural subunits of the Skp1-based and elongin B/C-based families of ubiquitin protein ligases). The NEDD8 pathway is essential for cell viability in fission yeast, and in mammalian cells it is essential for cell cycle progression and morphogenesis.

Product Information

Stock: 
PBS pH 7.4, 20% glycerol.
Antigen: 
Full-length, highly purified human UbcH12 protein.
Supplied: 
Protein A affinity purified rabbit polyclonal antibody.

Use & Storage

Use: 

Recommended dilution range for Western blot analysis is 1:100 to 1:1000.

Storage: 

Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.

Literature

References: 

Amir R.E., et al. (2002) J. Biol. Chem. 277: 23253-23259
Gong L., et al. (1999) J. Biol. Chem. 274: 12036-12042
Hochstrasser M. (2000) Nat. Cell. Biol. 2: E153-E157
Osaka F., et al. (1998) Genes. Dev. 12:2263-2268
Podust V.N., et al. (2000) Proc. Natl. Acad. Sci. 97:4579-4584
Tateishi K., et al. (2001) J. Cell. Biol. 155:571-580
Wada H., et al. (2000) J. Biol. Chem. 275: 17008-17015