Anti-UbcH9

Catalog #: A-645 UbcH9 is a member of the E2 family and is homologous to ubiquitin-conjugating enzymes, but is specific for the conjugation of SUMO to a variety of target proteins. This E2 is unusual in that it interacts directly with protein substrates that are modified by SUMOylation, and may play a role in substrate recognition. UbcH9 can mediate the conjugation of the SUMO-1 to a variety of proteins including RanGAP1, IκBα, and PML without the requirement of an E3 ligase.

Product Information

Quantity: 

200 μl

Price: 

$150

Stock: 

PBS pH 7.4.

Antigen: 

Full-length, highly purified human UbcH9 protein.

Use & Storage

Storage: 

Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.

Specificity: 

Recommended dilution range for Western blot analysis is 1:100 to 1:1000.

Literature

References: 

Desterro J.M., et al. (1999) J. Biol. Chem. 274:10618-24
Joana M.P, et al. (1997) FEBS. Lett. 417:3168-3179
Johnson E.S and Blobel G. (1997) J. Biol. Chem. 272:26799-26794
Ito K., et al. (1999) Cytogenet. Cell. 84:99-104
Liu Q., et all. (1999) J. Biol. Chem. 274:16979-16987
Matushewski K., et al. (1996) J. Biol. Chem. 271:2789-2794
Sampson D.A., et al. (2001) J. Biol. Chem. 276:21664-21669
Sampson D.A., et al. (2001) J. Biol. Chem. 276:21664-21669
Tatham M.H., et al. (2003) Biochem. 42:3168-3179