A-615

Material Data Sheet

UbcH5/UBE2D Polyclonal Ab

Price: $150.00
Catalog #: A-615

UbcH5/UBE2D Polyclonal Ab

Price: 
$150
Quantity: 
200 μl
Data Sheet: 
application/pdf iconA-615_antiH5.pdf
Stock: 

PBS pH 7.4, 20% glycerol.

Antigen: 
Full-length, highly purified human Ubch5 protein. The antibody recognizes isoforms UbcH5a, UbcH5b and UbcH5c.
Supplied: 

Protein A affinity purified rabbit polyclonal antibody.

UbcH5 enzymes are human homologs of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. UbcH5a mediates the degradation of a myriad of short-lived regulatory proteins (such as p53 in the presence of E6/E6-AP or MDM2, c-Fos, IκBα, p105) and abnormal proteins. UbcH5a has 89% and 88% sequence identity with UbcH5b and UbcH5c respectively.
Storage: 
Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.
Specificity: 
Recommended dilution range for Western blot analysis is 1:100 to 1:1000.</p>

Literature

References: 

Jensen J., et al. (1995) J. Biol. Chem. 270:30408-30414
Gehrke S.G., et al. (2003) Blood. 101:3288-3293
Nuber U. And Scheffner M. (1999) J. Biol. Chem. 274:7576-7582
Scheffner M., et al. (1994) Proc. Natl. Acad. Sci. 91:8797-8801
Schwarz S.E., et al. (1998) J. Biol. Chem. 273:12148-12154