A-610

Material Data Sheet

UbcH3/Cdc34

Price: $159.00
Catalog #: A-610

UbcH3/Cdc34

Price: 
$159
Quantity: 
200 µL
UbcH3 plays an essential role in the progression of cells from the G1 to S phase of the cell division cycle. One pathway (requiring Cdc34) initiates DNA replication by degrading a CDK (cyclin-dependent kinase) inhibitor. The second pathway, involves the anaphase-promoting complex (APC) which initiates chromosome egregation and exit from mitosis by degrading anaphase inhibitors and mitotic cyclins.

Product Information

Stock: 
PBS pH 7.4, 20% glycerol.
Antigen: 
Full-length, highly purified human UbcH3 protein.
Supplied: 
Protein A affinity purified rabbit polyclonal antibody.

Use & Storage

Use: 

Recommended dilution range for Western blot analysis is 1:100 to 1:1000.

Storage: 

Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.

Literature

References: 

Goebl M.G., et al. (1988) Science 241:1331-1335
Gonen H., et al. (1999) J. Biol. Chem. 274:14823-14830
King R. W., et al. (1996) Science 274:1652-1659
Listwan J., et al. (1998) EMBO. J. 17:368-383
Pintard L., et al. (2003) Nat. Cell. Biol. 5:856-857
Plon S.E., et al. (1993) Proc. Natl. Acad. Sci. 90:10484-10488
Ptak C., et al. (1994) J. Biol. Chem. 269:26539-26545
Seol J.H., et al. (1999) Gene. Dev. 13:1614-1626
Varelas X., et al. (2003) Mol. Cell. Biol. 23:5388-5400