A-603

Material Data Sheet

UbcH1/UBE2K/E2-25K

Price: $159.00
Catalog #: A-603

UbcH1/UBE2K/E2-25K

Price: 
$159
Quantity: 
200 µL
Biochemically, UBE2K (E2-25K) can catalyze the formation of K48-linked ubiquitin chains in the absence of an E3 ligase. This enzyme has a unique C-terminal UBA domain that plays a critical role in E1 selectivity and chain synthesis. Recently, UBE2K (or E2-25K or HIP2) has been shown to bind to the N- terminus of huntington and may play a role in Huntington’s disease.

Product Information

Stock: 
PBS pH 7.4, 20% glycerol
Antigen: 
Full-length, highly purified human UbcH1 protein.
Supplied: 
Protein A affinity purified rabbit polyclonal antibody.

Use & Storage

Use: 

Recommended dilution range for Western blot analysis is 1:100 to 1:1000.

Storage: 

Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.

Literature

References: 

Chen Z.J., et al. (1991) J. Biol. Chem. 266:15698-15704
Haldeman M.T., et al. (1997) Biochem. 36:10526-10537
Kalchman M.A.., et al. (1996) J. Biol. Chem. 271:19385-19394
Mastrandrea L.D., et al. (1998) Biochem. 37:9784-9792
Pickart C.M., et al. (1992) J. Biol. Chem. 267:14418-14423
Piotrowski J., et al. (1997) J. Biol. Chem. 272:23712-23721
Song S., et al. (2003) Mol. Cell 12:553-563
Yao T. and Cohen R.E. (2000) J. Biol. Chem. 275:36862-36868