A-306

Material Data Sheet

UBE1L/ISG15 E1 Polyclonal Ab

Price: $220.00
Catalog #: A-306

UBE1L/ISG15 E1 Polyclonal Ab

Price: 
$220
Quantity: 
100 μl
Data Sheet: 
application/pdf icon306-ube1lisg15-e1-polyclonal-ab.pdf
This E1 is responsible for the first step in ISG15-protein isopeptide bond formation. This enzyme catalyzes the activation of the C-terminal carboxyl group of ISG15 by forming a high-energy thioester bond in an ATP-dependent manner. The activated ISG15 is then transferred to UbcH8 and subsequently to lysine groups of target proteins via this thiolester conjugation cascade. The E1 enzyme is a critical component for the initiation of any in vitro conjugation reactions.

Product Information

Stock: 
34 mg/ml antiserum. No preservatives added.
Immunogen: 
Synthetic peptide D<sup>222</sup>CDPRSIHVREDGSLEIGD<sup>240</sup> of N-terminus of UBE1L&nbsp;
Reactivity: 
Antibody detects human UBE1L protein (112 kDa).

Use & Storage

Use: 

Functional for Western blotting and Immunohistochemistry.

Storage: 
Store vial at -20°C. Avoid freeze-thaw cycles.

Literature

References: 

Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95
Kitareenwan S., et al. (2002) Proc. Natl. Acad. Sci. 99:3806-3811
Kok K., et al. (1994) Canc. Res. 54:4183-4187
Pitha-Rowe, I., et al. (2004) J. Biol. Chem. 279:18178-18187
Pitha-Rowe, I., et al. (2004) Canc. Res. 264:8109-8115
Yuan W., et al. (2001) EMBO. J. 20: 362-371