Anti-UBE1L ISG15 E1

Catalog #: A-306 This E1 is responsible for the first step in ISG15-protein isopeptide bond formation. This enzyme catalyzes the activation of the C-terminal carboxyl group of ISG15 by forming a high-energy thioester bond in an ATP-dependent manner. The activated ISG15 is then transferred to UbcH8 and subsequently to lysine groups of target proteins via this thiolester conjugation cascade. The E1 enzyme is a critical component for the initiation of any in vitro conjugation reactions.

Product Information

Quantity: 

100 μl

Price: 

$220

Stock: 

34 mg/ml antiserum. No preservatives added.

Host: 

Rabbit Polyclonal

Immunogen: 

Synthetic peptide D²²²CDPRSIHVREDGSLEIGD²⁴⁰ of N-terminus of UBE1L

 

Reactivity: 

Antibody detects human UBE1L protein (112 kDa).

Use & Storage

Storage: 

Store vial at -20°C. Avoid freeze-thaw cycles.

Specificity: 

Functional for Western blotting and Immunohistochemistry.

Literature

References: 

Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95
Kitareenwan S., et al. (2002) Proc. Natl. Acad. Sci. 99:3806-3811
Kok K., et al. (1994) Canc. Res. 54:4183-4187
Pitha-Rowe, I., et al. (2004) J. Biol. Chem. 279:18178-18187
Pitha-Rowe, I., et al. (2004) Canc. Res. 264:8109-8115
Yuan W., et al. (2001) EMBO. J. 20: 362-371