1334

Material Data Sheet

Bafilomycin A1

Price: $129.00
Catalog #: 1334

Bafilomycin A1

Price: 
$119
Quantity: 
10 µg
Data Sheet: 

Chemical Name: (3Z,5E,7R,8S,9S,11E,13E,15S,16R)-8-­Hydroxy-16-[(1S,2R,3S)-2-hydroxy-1-methyl-3-[(2R,4­R,5S,6R)-tetrahydro-2,4-dihydroxy-5-methyl-6-(1-me­thylethyl)-2H-pyran-2-yl]butyl]-3,15-dimethoxy-5,7­,9,11-tetramethyloxacyclohexadeca-3,5,11,13-tetrae­n-2-one

Biological Activity

Highly potent, selective inhibitor of vacuolar H+-ATPases (IC50 = 0.6 - 1.5 nM in bovine chromaffin granules). Selective for v-ATPase over other ATP hydrolyzing enzymes such as F-ATPases and the H+/K+-ATPase (P-ATPase). Thought to inhibit autophagy either by blocking autophagosome-lysosome fusion (in H4IIE cells), or by blocking lysosomal degradation.

Product Information

MW: 
622.84
Purity: 
>98%
Solubility: 
Soluble in DMSO
Storage: 
Desiccate at -20°C

Literature

References: 

Yamamoto et al (1998) Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion between autophagosomes and lysosomes in rat hepatoma cell line, H-4-II-E cells. Cell Struct.Funct. 23 33. PMID: 9639028.

Gagliardi et al (1999) Chemistry and stucture activity relationships of bafilomycin A1, a potent and selective inhibitor of the vacuolar H+-ATPase. Curr.Med.Chem. 6 1197. PMID: 10519916.

Fass et al (2006) Microtubules support production of starvation-induced autophagosomes but not their targeting and fusion with lysosomes. J.Biol.Chem. 281 36303. PMID: 16963441.