A-110

New
Material Data Sheet

Phospho-Ubiquitin (pS65), PAb

Price: $295.00
Catalog #: A-110

Phospho-Ubiquitin (pS65), PAb

Price: 
$295
Quantity: 
50 µg
Data Sheet: 

Serine/Threonine kinase PINK1 (PTEN-induced putative kinase protein 1) plays a critical role in preventing mitochondrial dysfunction during cellular stress.  PINK is translated in the cytosol, then translocated to the outer mitochondrial membrane where it is rapidly cleaved and degraded as a part of normal mitochondrial function.  In damaged (depolarized) mitochondria PINK becomes stabilized and accumulates, resulting in the subsequent phosphorylation of numerous proteins on the mitochondrial surface including Mfn2.  Ultimately PARK2 (E3 Ubiquitin Ligase Parkin) is recruited to the damaged mitochondria where it is activated by PINK-mediated phosphorylation of PARK2 at serine 65, and PARK2 interaction with phosphorylated Ubiquitin (also phosphorylated by PINK on serine 65).  This signaling cascade is critical for clearing the damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2.

Product Information

Stock: 
0.5 mg/ml in PBS pH 7.4, 50% Glyverol
Antigen: 
Peptide based on phosphorylated (serine 65) human Ubiquitin, Accession # P0CG47

Use & Storage

Use: 

Recommended concentration for Western Blot is 0.5 - 1 µg/ml.

Storage: 

Store at -20°C.  Storage at other temperatures is not recommended.

Specificity: 
This antibody detects mono- and Polyubiquitin chains that are phosphorylated on Serine 65. It has no cross-reactivity with non-phosphorylated Ubiquitin, Ubiquitin phosphorylated at Serine 57 or Tyrosine 59, or phosphorylated Parkin.