A-104

Material Data Sheet

Anti-Ubiquitin

Price: $195.00
Catalog #: A-104

Anti-Ubiquitin

Price: 
$195
Quantity: 
50 µg
Data Sheet: 

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast. In mammals, four ubiquitin genes encode for two ubiquitin­ribosomal fusion proteins and two polyubiquitin proteins.  Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa.  Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C-­terminal glycine residue of ubiquitin and a lysine residue in the target protein.  This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi­step process that requires three enzymes: a ubiquitin­activating (E1) enzyme, a ubiquitin­conjugating (E2) enzyme, and a ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP­dependent Proteolysis Factor 1 (APF­1).  In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post­endocytic sorting.  

Product Information

Stock: 
0.5  mg/mL in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide
Antigen: 
Human Ubiquitin+1 synthetic peptide

Use & Storage

Use: 

Recommended concentration for Western Blot is 0.1 - 0.5 µg/ml.

Storage: 

Store at -20°C.

Specificity: 
This antibody detects human Ubiquitin and Ubiquitin+1 in Western blots