A-101

Material Data Sheet

Anti-Ubiquitin K48 Linkage

Price: $239.00
Catalog #: A-101

Anti-Ubiquitin K48 Linkage

Price: 
$239
Quantity: 
50 µg
Data Sheet: 

Polyubiquitin chains are composed of ubiquitin monomers that are covalently linked through isopeptide bonds (other than linear, or “Met1-linked” polyubiquitin).  Isopeptides are formed between a lysine residue of one Ubiquitin molecule and the C-­terminal glycine residue of another Ubiquitin molecule. Seven of the seventy-six amino acids in ubiquitin are lysine residues that can participate in polyubiquitin chain formation.  Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes.  K48-linked polyubiquitin chains attached to substrate proteins often serve as a recognition sequence for targeting and destruction of the substrate by the 26S Proteasome. This antibody detects the K48 linkage. It does not detect monoubiquitin or ubiquitin liked via any other lysine residue.  Reactivity across all species is anticipated.

Product Information

Stock: 
0.5  mg/mL in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide

Use & Storage

Use: 

Recommended concentration for Western blot is 0.1-0.5 µg/ml.

Storage: 

Store at -20°C.

Specificity: 
This antibody detects endogenous, human proteins containing K48-linked polyubiquitin chains in Western blots. This antibody detects purified, recombinant K48-linked polyubiquitin chains, but has no cross-reactivity to monoubiquitin or polyubiquitin of other linkages.

Literature

References: 

Behrends, C. & J.W. Harper (2011) Nat. Struct. Mol. Biol. 18: 520. 

Greene, W. et al. (2012) PLoS Pathog. 8: e1002703. 

Henry, A.G. et al. (2012) Dev. Cell  23: 519. 

Scheffner, M. et al. (1995) Nature  373: 81. 

Sharp, P.M. & W.­H. Li (1987) Trends Ecol. Evol.  2: 328. 

Tong, X. et al. (2012) J. Biol. Chem.  287: 25280. 

Wei, W. et al. (2004) Nature  428: 194. 

Zhang, J. et al. (2012) J. Biol. Chem.  287: 28646.