0384

Material Data Sheet

N-Acetyl-L-leucyl-L-leucyl-L-methional

Price: $109.00
Catalog #: 0384

N-Acetyl-L-leucyl-L-leucyl-L-methional

Price: 
$109 $455
Quantity: 
10 mg  50 mg
Data Sheet: 
application/pdf icon0384-n-acetyl-l-leucyl-l-leucyl-l-methional.pdf

Peptide aldehydes form a covalent hemiacetal adduct with the 20S proteasome, inhibiting the chymotrypsin-like peptidase activity. These inhibitors are reversible and cell-permeable, and also inhibit the activity of calpains and cathepsins.

Product Information

Structure: 
Purity: 
> 95% by TLC, HPLC. Structure confirmed by NMR.
Solubility: 
Soluble in DMSO up to 15 mg/ml. Pellet dry compound prior to DMSO addition.

Use & Storage

Use: 

Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Pre-incubation with compound prior to assay is recommended for maximal effect. 20S proteasome Ki = 2 μM

Storage: 
Store DMSO stock at -20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Griscavage, J.M., et al. (1995) Biochem. Biophys. Res. Comm. 215:721729

Kisselev A.F., et al. (2003) J. Biol. Chem. 278:35869-35877

Mellgren R.L. (1997) J. Biol. Chem. 272:29899-29903

Myung J., et al. (2001) Med. Res. Rev. 4:245-273

Vinitsky A., et al. (1994) J. Biol. Chem. 269:29860-29860