Product References

Boston Biochem's products have been used successfully in hundreds of UPP-related publications. We have listed our most recent references below to provide our customers with an exceptional resource for product use information. The references are organized to mirror our product divisions for easy location. Products referenced in each article are listed below in red. Please inquire to request archives as we have curated references back to 2003.

Substrate Proteins

Neilsen, P.M. et al. (2012) "New 26S Proteasome Inhibitors with High Selectivity for Chymotrypsin-Like Activity and p53-Dependent Cytotoxicity." ACS Chem. Biol.

PubMed ID: 23190346 | Product(s) Referenced: purified rabbit20S proteasome, Suc-LLVY-AMC

Zhu, Y. et al. (2012) "Synthesis and inhibitory activities against colon cancer cell growth and proteasome of alkylresorcinols." J. Agric. Food Chem. 60(35): 8624–8631.

PubMed ID: 22897570 | Product(s) Referenced: human 20S proteasome, proteasome activator PA28, Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY-AMC)

Galisson, F., et al. (2011) “A Novel Proteomics Approach to Identify SUMOylated Proteins and Their Modification Sites in Human Cells.” Molecular and Cellular Proteomics, 10: M110.004796.

PubMed ID: 21098080 | Product(s) Referenced: E2-25K

Wu, T., et al. (2010) “UBE2S drives elongation of K11-linked ubiquitin chains by the Anaphase-Promoting Complex.” PNAS 107: 1355 - 1360.

PubMed ID: 20080579 | Product(s) Referenced: E2-25K

Kim, H.T., et al. (2009) "S5a promotes protein degradation by blocking synthesis of non-degradable forked ubiquitin chains." EMBO J. 28: 1867-1877.

Gho, A.M., et al. (2008). "Components of the ubiquitin-proteasome pathway compete for surfaces on Rad23 family proteins." BMC Biochem. 9:4.

PubMed ID: 18234089 | Product(s) Referenced: E2-25K

Yamada, M., J. Ohnishi, et al. (2006). "NARF, an emo-like Kinase (NLK)-associated Ring Finger Protein Regulates the Ubiquitylation and Degradation of T Cell Factor/Lymphoid Enhancer Factor (TCF/LEF)." J. Biol. Chem. 281(30): 20749-20760.

PubMed ID: 16714285 | Product(s) Referenced: E2-25K

SUMO

Chicooree, N. et al. (2013) "A novel approach to the analysis of SUMOylation with the independent use of trypsin and elastase digestion followed by database searching utilising consecutive residue addition to lysine." Rapid Commun. Mass Spectrom. 27(1): 127–134.

PubMed ID: 23239325 | Product(s) Referenced: di-Ub-K48, di-Ub-K63, di-SUMO-2, di-SUMO-3
PubMed ID: 23751493 | Product(s) Referenced: poly-SUMO chains (3-8)
PubMed ID: 23751493 | Product(s) Referenced: SAE1/2
PubMed ID: 23751493 | Product(s) Referenced: SUMO1
PubMed ID: 23751493 | Product(s) Referenced: SUMO2
PubMed ID: 23751493 | Product(s) Referenced: SUMO2
PubMed ID: 23547032 | Product(s) Referenced: SUMO
PubMed ID: 23673342 | Product(s) Referenced: His6-SUMO2
PubMed ID: 23673342 | Product(s) Referenced: SAE1/SAE2
PubMed ID: 23673342 | Product(s) Referenced: SUMO1-K16R
PubMed ID: 23673342 | Product(s) Referenced: SUMO1-VS
PubMed ID: 23673342 | Product(s) Referenced: SUMO2-K11R
PubMed ID: 23888040 | Product(s) Referenced: SUMO1
PubMed ID: 23943332 | Product(s) Referenced: di-SUMO(2/3) protein substrates
PubMed ID: 23990779 | Product(s) Referenced: His-SUMO-1
PubMed ID: 23990779 | Product(s) Referenced: His-SUMO-2
PubMed ID: 23990779 | Product(s) Referenced: His-SUMO-3

Osula, O. et al. (2012) "Identification of protein SUMOylation sites by mass spectrometry using combined microwave-assisted aspartic acid cleavage and tryptic digestion." J Mass Spectrom 47(5): 644–654.

PubMed ID: 22576878 | Product(s) Referenced: di-SUMO-2, di-SUMO-3

Liu, Y. et al. (2012) "NF-κB Repression by PIAS3 Mediated RelA SUMOylation." PLoS ONE 7(5): e37636.

PubMed ID: 22649547 | Product(s) Referenced: SAE1/SAE2

Luo, K. et al. (2012) "Sumoylation of MDC1 is important for proper DNA damage response." The EMBO journal.  2012 May 25;

PubMed ID: 22635276 | Product(s) Referenced: SUMO-activating enzyme (UBA2), SUMO1, SUMO2, SUMO3

Rendtlew Danielsen, J. et al. (2012) "DNA damage-inducible SUMOylation of HERC2 promotes RNF8 binding via a novel SUMO-binding Zinc finger." J. Cell Biol. 197(2): 179–187.

PubMed ID: 22508508 | Product(s) Referenced: SUMO1, SUMO2

Guo, Z. et al. (2012) "Sequential Posttranslational Modifications Program FEN1 Degradation during Cell-Cycle Progression." Molecular cell.

PubMed ID: 22749529 | Product(s) Referenced: ubiquitination reaction buffer, UBE1, UBE2M, HeLa S100 fraction, SUMOylation kits, ubiquitination kit

Hu, X. et al. (2012) "Rap80 Protein Recruitment to DNA Double-strand Breaks Requires Binding to Both Small Ubiquitin-like Modifier (SUMO) and Ubiquitin Conjugates." J. Biol. Chem. 287(30): 25510–25519.

PubMed ID: 22689573 | Product(s) Referenced: SUMO2 3– 8, agarose-SUMO2 beads, Ub Lys-63 2–7,

Cotto-Rios, X.M. et al. (2012) "Deubiquitinases as a signaling target of oxidative stress." Cell Rep 2(6): 1475–1484.

PubMed ID: 23219552 | Product(s) Referenced: UbVME, recombinant ubiquitin chain K48-linked, UCH-L1, SENP1-CD, STAM, recombinant ubiquitin chain K63-linked, SUMO1-vs, USP7, AMSH,

Chang, C., et al (2011) “A Novel Cyclic AMP/Epac1/CaMKI Signaling Cascade Promotes GCM1 Desumoylation and Placental Cell Fusion.” Mol. Cell. Biol. 31: 3820 - 3831.

PubMed ID: 21791615 | Product(s) Referenced: His-SUMO1