Product References

Boston Biochem's products have been used successfully in hundreds of UPP-related publications. We have listed our most recent references below to provide our customers with an exceptional resource for product use information. The references are organized to mirror our product divisions for easy location. Products referenced in each article are listed below in red. Please inquire to request archives as we have curated references back to 2003.

E3 Ligase

Cotto-Rios, X.M. et al. (2012) "Deubiquitinases as a signaling target of oxidative stress." Cell Rep 2(6): 1475–1484.

PubMed ID: 23219552 | Product(s) Referenced: UbVME, recombinant ubiquitin chain K48-linked, UCH-L1, SENP1-CD, STAM, recombinant ubiquitin chain K63-linked, SUMO1-vs, USP7, AMSH,

Kalia, L.V., et al. (2011) “Ubiquitinylation of [alpha]-Synuclein by Carboxyl Terminus Hsp70-Interacting Protein (CHIP) Is Regulated by Bcl-2-Associated Athanogene 5 (BAG5).” PloS one, 6: e14695. doi:10.1371/journal.pone.0014695 .

PubMed ID: 21358815 | Product(s) Referenced: HDM2, MuRF1

Bosanac, I., et al. (2011) “Modulation of K11-Linkage Formation by Variable Loop Residues within UbcH5A.” Journal of Mol. Bio.: doi:10.1016/j.jmb.2011.03.011.* .

PubMed ID: 21396940 | Product(s) Referenced: MURF1

Shin, J., et al. (2011) “PARIS (ZNF746) Repression of PGC-1[alpha] Contributes to Neurodegeneration in Parkinson's Disease.” Cell, 144: 689-702.

PubMed ID: 21376232 | Product(s) Referenced: His-Parkin

Jin, X, et al. (2010) “An Atypical E3 Ligase Zinc Finger Protein 91 Stabilizes and Activates NF-B-inducing Kinase via Lys63-linked Ubiquitination.” J. Biol. Chem. 285: 30539-30547.

PubMed ID: 20682767 | Product(s) Referenced: Lys63-only ubiquitin

Kaneko, M., et al. (2010) “Loss of HRD1-Mediated Protein Degradation Causes Amyloid Precursor Protein Accumulation and Amyloid-[beta] Generation.” J. Neurosci., 3924 - 3932.

PubMed ID: 20237263 | Product(s) Referenced: RP-HRD1 (E3)

Chymkowitch, P., et al. (2010) "The phosphorylation of the androgen receptor by TFIIH directs the ubiquitin/proteasome process.” The EMBO Journal 30(3): 468–479.

PubMed ID: 21157430 | Product(s) Referenced: MDM2

Fu, W., et al. (2009) "MDM2 Acts Downstream of p53 as an E3 Ligase to Promote FOXO Ubiquitination and Degradation." J. Biol. Chem. 284: 13987-14000.

PubMed ID: 19321440 | Product(s) Referenced: MDM2

Siepe, D. and Jentsch, S. (2009) "Prolyl isomerase Pin1 acts as a switch to control the degree of substrate ubiquitylation.” Nature Cell Biology 11(8): 967–972.

PubMed ID: 19597489 | Product(s) Referenced: recombinant E3 (His6-Mdm2)

Newton, K., et al. (2008) "Ubiquitin Chain Editing Revealed by Polyubiquitin Linkage-Specific Antibodies.” Cell 134(4): 668–678.

PubMed ID: 18724939 | Product(s) Referenced: MuRF1 autoubiquitination reactions

Hrecka, K., M. Gierszewska, et al. (2007). "Lentiviral Vpr usurps Cul4-DDB1[VprBP] E3 ubiquitin ligase to modulate cell cycle." PNAS 104(28): 11778-11783.

PubMed ID: 17609381

Fortier, J. M. and J. Kornbluth (2006). "NK Lytic-Associated Molecule, Involved in NK Cytotoxic Function, Is an E3 Ligase." J. Immunol. 176(11): 6454-6463.

Product(s) Referenced: Polyclonal UbcH8 Ab

Dai, M.-S., D. et al. (2006). "Regulation of the MDM2-p53 Pathway by Ribosomal Protein L11 Involves a Post-ubiquitination Mechanism." J. Biol. Chem. 281(34): 24304-24313.

PubMed ID: 16803902 | Product(s) Referenced: purified human recombinant E2 (UbcH5a)

Burstein E., et al. (2004) "A novel role for XIAP in copper homeostasis through regulation of MURR1." EMBO J. 23(1): 244-254.

PubMed ID: 14685266 | Product(s) Referenced: E3

Fractions

PubMed ID: 24170810 | Product(s) Referenced: HeLa cytoplasmic extract (S-100 fraction)
PubMed ID: 23689986 | Product(s) Referenced: Fraction II HeLa Degradation Kit

Herr, R.A. et al. (2012) "Newly Discovered Viral E3 Ligase pK3 Induces Endoplasmic Reticulum-associated Degradation of Class I Major Histocompatibility Proteins and Their Membrane-bound Chaperones." J. Biol. Chem. 287(18): 14467–14479.

PubMed ID: 22403403 | Product(s) Referenced: deubiquiting enzyme inhibitor ubiquitin aldehyde, proteasome inhibitor MG132

Herr, R.A. et al. (2012) "Newly Discovered Viral E3 Ligase pK3 Induces Endoplasmic Reticulum-associated Degradation of Class I Major Histocompatibility Proteins and Their Membrane-bound Chaperones." J. Biol. Chem. 287(18): 14467–14479.

PubMed ID: 22403403 | Product(s) Referenced: Fraction II (FII) from rabbit reticulocyte extract

Watanabe, T. et al. (2012) "FBL2 regulates amyloid precursor protein (APP) metabolism by promoting ubiquitination-dependent APP degradation and inhibition of APP endocytosis." J. Neurosci. 32(10): 3352–3365.

PubMed ID: Fraction II Reticulocyte Conjugation kit | Product(s) Referenced: 22399757

Kensche, T. et al. (2012) "Analysis of Nuclear Factor-κB (NF-κB) Essential Modulator (NEMO) Binding to Linear and Lysine-linked Ubiquitin Chains and Its Role in the Activation of NF-κB." J. Biol. Chem. 287(28): 23626–23634.

PubMed ID: 22605335 | Product(s) Referenced: creatine phosphokinase, HeLa-S-100 fraction II, ubiquitin, ubiquitin aldehyde

Zhang, S. et al. (2012) "GST:Identification of RNF8 as a ubiquitin ligase involved in targeting the p12 subunit of DNA polymerase δ for degradation in response to DNA damage." J. Biol. Chem.

PubMed ID: 23233665 | Product(s) Referenced: ATP regenerating solution (ENS), ubiquitin aldehyde, HeLa S100 fraction, Recombinant human E1 ubiquitin activating enzyme, Recombinant human ubiquitin, Recombinant human ubiquitin K48R, Recombinant human ubiquitin K63R, Recombinant human UbcH5c, Recombinant human Cdc34, Recombinant human UbcH2

Arun, P., et al. (2011) “Senescence Marker Protein 30 (SMP30) Expression in Eukaryotic Cells: Existence of Multiple Species and Membrane Localization.” PLoS one, 6: e16545. doi:10.1371/journal.pone.0016545.

PubMed ID: 21347421 | Product(s) Referenced: S-100 Hela degradation mixture

Zhu, F., et al. (2011) “Phosphorylation of H2AX at Ser139 and a New Phosphorylation Site Ser16 by RSK2 Decreases H2AX Ubiquitination and Inhibits Cell Transformation.” Cancer Research, 71: 393.

PubMed ID: 21224359 | Product(s) Referenced: Fraction II (FII)-HeLa

Bhandari, D., et al. (2011) “Ubiquitination of mRNA cycling sequence binding protein from Leishmania donovani (LdCSBP) modulates the RNA endonuclease activity of its Smr domain.” FEBS Letters, 585: 809-813.

Product(s) Referenced: S-100 HeLa conjugation kit

Chaugule, V.K., et al. (2011) "Autoregulation of Parkin activity through its ubiquitin-like domain.” The EMBO Journal 30(14): 2853–2867.

PubMed ID: 21694720 | Product(s) Referenced: HeLa S100 fraction

Burnett, B.G., et al. (2009) "Regulation of SMN Protein Stability." Mol. Cell. Biol. 29: 1107-1115.

PubMed ID: 19103745 | Product(s) Referenced: ubiquitin conjugation enzyme fraction

Marteijn, J., et al. (2009) "The ubiquitin ligase Triad1 inhibits myelopoiesis through UbcH7 and Ubc13 interacting domains.” Leukemia 23(8): 1480–1489.

PubMed ID: 19340006 | Product(s) Referenced: reticulocyte fraction IIA

Wang, X., et al. (2009) “Ube2j2 ubiquitinates hydroxylated amino acids on ER-associated degradation substrates.” J. Cell Biol. 187: 655 - 668.

PubMed ID: 19951915 | Product(s) Referenced: FII from rabbit reticulocyte extract

Chiang M-H., et al. (2008) "Ubiquitin-Conjugating Enzyme UBE2D2 Is Responsible for FBXW2 (F-Box and WD Repeat Domain Containing 2)-Mediated Human GCM1 (Glial Cell Missing Homolog 1) Ubiquitination and Degradation." Biol Reprod 79(5):914-920.

PubMed ID: 18703417 | Product(s) Referenced: Recombinant CDC34

Liu, J., et al. (2008) "A therapeutic dose of doxorubicin activates ubiquitin-proteasome system-mediated proteolysis by acting on both the ubiquitination apparatus and proteasome." Am. J. Physiol. Heart.Circ. Physiol. 295(6): H2541-H2550.

PubMed ID: 18978187 | Product(s) Referenced: ubiquitin conjugation enzymes (F-340)

Todi, S. V., et al. (2007). "Cellular turnover of the polyglutamine disease protein ataxin-3 is regulated by its catalytic activity." J. Biol. Chem.: 282(40): 29348-29358.

PubMed ID: 17693639 | Product(s) Referenced: rabbit 26 S fractions

Zhu, F., et al. (2006). "COOH-Terminal Src Kinase-Mediated c-Jun Phosphorylation Promotes c-Jun Degradation and Inhibits Cell Transformation." Cancer Res. 66(11): 5729-5736.

PubMed ID: 16740711 | Product(s) Referenced: Fraction II HeLa Conjugation Kit

Doss-Pepe E. W., et al. (2005) "[alpha]-Synuclein and Parkin Contribute to the Assembly of Ubiquitin Lysine 63-linked Multiubiquitin Chains." J. Biol. Chem. 280(17): 16619-16624.

PubMed ID: 15718234 | Product(s) Referenced: Fraction II

Kaneko-Oshikawa C., et al. (2005) "Mammalian E4 Is Required for Cardiac Development and Maintenance of the Nervous System." Mol. Cell. Biol. 25(24): 10953-10964.

PubMed ID: 16314518 | Product(s) Referenced: S100 lysate protein

Marteijn J. A. F., et al. (2005) "The E3 ubiquitin-protein ligase Triad1 inhibits clonogenic growth of primary myeloid progenitor cells." Blood 106(13): 4114-4123.

PubMed ID: 16118314 | Product(s) Referenced: http://bloodjournal.hematologylibrary.org/content/106/13/4114.long

Song B.-L. and DeBose-Boyd R.A. (2004) "Ubiquitination of 3-Hydroxy-3-methylglutaryl-CoA Reductase in Permeabilized Cells Mediated by Cytosolic E1 and a Putative Membrane-bound Ubiquitin Ligase." J. Biol. Chem. 279(27): 28798-28806.

PubMed ID: 15090540 | Product(s) Referenced: Fraction I, Fraction II, S100 isolated from HeLa cells

Sampathu, D.M., et al. (2003) "Ubiquitination of [alpha]-synuclein is not required for formation of pathological inclusions in [alpha]--Synucleinopathies." Am. J. Path. 163: 91-100.

PubMed ID: 12819014 | Product(s) Referenced: ubiquitin conjugation fractions A and B (derived from mammalian cell lysate)

Shim, M., et al. (2003). "Lithium stabilizes the CCAAT/Enhancer-binding protein [alpha] (C/EBP[alpha]) through a glycogen synthase kinase 3 (GSK3)-independent pathway involving direct inhibitor of proteasomal activity." Genes Cells 11(7): 731-744.

PubMed ID: 12668682 | Product(s) Referenced: HeLa S-100 fraction

Ohh, M., et al. (2002). "An intact NEDD8 pathway is required for Cullin-dependent ubiquitylation in mammalian cells.” EMBO Reports 3(2): 177-182.

PubMed ID: 11818338 | Product(s) Referenced: HeLa FII

Inhibitor

PubMed ID: 24273049 | Product(s) Referenced: SucLLVY-AMC
Product(s) Referenced: MG132
PubMed ID: 24158907 | Product(s) Referenced: MG132
PubMed ID: 23475958 | Product(s) Referenced: ubiquitin aldehyde
PubMed ID: 23318177 | Product(s) Referenced: 19S inhibitor b-AP15
PubMed ID: epoximycin | Product(s) Referenced: 23437325
PubMed ID: 23535459 | Product(s) Referenced: MG132
PubMed ID: 23486064 | Product(s) Referenced: ubiquitin aldehyde
PubMed ID: 23424652 | Product(s) Referenced: HA-Ub vinyl sulfone
PubMed ID: 23458873 | Product(s) Referenced: MG-132 proteosome inhibitor
PubMed ID: 23541081 | Product(s) Referenced: MG-132