Product References

Boston Biochem's products have been used successfully in hundreds of UPP-related publications. We have listed our most recent references below to provide our customers with an exceptional resource for product use information. The references are organized to mirror our product divisions for easy location. Products referenced in each article are listed below in red. Please inquire to request archives as we have curated references back to 2003.

Enzymes

PubMed ID: 24069405 | Product(s) Referenced: recombinant yeast E1 (Ube1)
PubMed ID: 23962426 | Product(s) Referenced: recombinant human E1
PubMed ID: 24076975 | Product(s) Referenced: yeast E1
PubMed ID: 23885119 | Product(s) Referenced: E1
PubMed ID: 23861750 | Product(s) Referenced: E1
PubMed ID: 24043303 | Product(s) Referenced: Ube1

ISG15

Basters, A. et al. (2012) "High yield expression of catalytically active USP18 using a Trigger Factor fusion system." BMC Biotechnol. 12(1): 56.

PubMed ID: 22916876 | Product(s) Referenced: HA-ISG15 vinyl sulfone (ISG15-VS), ISG15-amidomethyl coumarin (AMC), HA-Ubiquitin (Ub-VS)

Na, C.-H. et al. (2012) "Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis." J. Proteome Res. 11(9):4722-32.

PubMed ID: 22871113 | Product(s) Referenced: K48 di-Ub, recombinant purified ISG15, recombinant purified Nedd8, recombinant purified Ub

Capodagli, G., et al. (2011) “Structural analysis of a viral ovarian tumor domain protease from the Crimean-Congo Hemorrhagic Fever virus in complex with covalently bonded ubiquitin.” Journal of Virology, 85: 3621-3630.

PubMed ID: 21228232 | Product(s) Referenced: ISG15-AMC

James, T., et al. (2011) “Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease.” PNAS, 108: 2222-2227.

PubMed ID: 21245344 | Product(s) Referenced: ISG15–AMC

Malakhova O.A. and Zhang D-E. (2008) "ISG15 Inhibits Nedd4 Ubiquitin E3 Activity and Enhances the Innate Antiviral Response." J. Biol. Chem. 283: 8783-8787.

PubMed ID: 18287095 | Product(s) Referenced: ISG15

Ratia, K, et al. (2008). "A non-covalent class of papain-like protease/deubiquitinase inhibitors blocks SARS virus replication." PNAS 105 (42): 16119-16124 .

PubMed ID: 18852458 | Product(s) Referenced: ISG15-AMC

Catic A., et al. (2007) "Screen for ISG15-cross-reactive Deubiquitinases." PLoS ONE 2(7): E769.

PubMed ID: 17653289 | Product(s) Referenced: ISG15

Affinity Matrices / Proteins

Hu, X. et al. (2012) "Rap80 Protein Recruitment to DNA Double-strand Breaks Requires Binding to Both Small Ubiquitin-like Modifier (SUMO) and Ubiquitin Conjugates." J. Biol. Chem. 287(30): 25510–25519.

PubMed ID: 22689573 | Product(s) Referenced: SUMO2 3– 8, agarose-SUMO2 beads, Ub Lys-63 2–7,

Durcan, T.M., et al. (2011) “The Machado–Joseph disease-associated mutant form of ataxin-3 regulates parkin ubiquitination and stability.” Human Molecular Genetics, 20: 141-154.

PubMed ID: 20940148 | Product(s) Referenced: His-ataxin-3 (14Q)

Gerlach, B., et al. (2011) "Linear ubiquitination prevents inflammation and regulates immune signalling.” Nature 471(7340): 591–596.

PubMed ID: 21455173 | Product(s) Referenced: agarose beads coupled to ubiquitin

LaLonde, D. and Bretscher, A. (2011) “The UBX Protein SAKS1 Negatively Regulates Endoplasmic Reticulum-associated Degradation and p97-dependent Degradation.” The Journal of Biological Chemistry, 286: 4892-4901.

PubMed ID: 21135095 | Product(s) Referenced: His6-tagged Ataxin-3

Sun, H., et al. (2011) “Bcr-Abl ubiquitination and Usp9x inhibition block kinase signaling and promote CML cell apoptosis.” Blood, 117: 3151-3162.

PubMed ID: Bcr-Abl ubiquitination and Usp9x inhibition block kinase signaling and promote CML cell apoptosis. | Product(s) Referenced: ataxin-agarose beads, Rap80-agarose beads

Feng, L., et al. (2010) “The Lys63-specific Deubiquitinating Enzyme BRCC36 Is Regulated by Two Scaffold Proteins Localizing in Different Subcellular Compartments.” J. Biol. Chem. 285: 30982-30988.

PubMed ID: 20656690 | Product(s) Referenced: RAP80 UIM-agarose beads

Liang, J., et al. (2010) “MCP-induced protein 1 deubiquitinates TRAF proteins and negatively regulates JNK and NF-B signaling.” J. Exp. Med. 207: 2959-2973.

PubMed ID: 21115689 | Product(s) Referenced: ubiquitin-agarose

De Schutter, J., et al. (2009) “SHIP2 (SH2 Domain-containing Inositol Phosphatase 2) SH2 Domain Negatively Controls SHIP2 Monoubiquitination in Response to Epidermal Growth Factor.” J. Biol. Chem. 284: 36062 - 36076.

PubMed ID: 19880507 | Product(s) Referenced: ubiquitin-agarose beads

Lis, E. T. and F. E. Romesberg (2006). "Role of Doa1 in the Saccharomyces cerevisiae DNA Damage Response." Mol. Cell. Biol. 26(11): 4122-4133.

PubMed ID: 16705165 | Product(s) Referenced: ubiquitin agarose

Kowanetz K., et al. (2004) "Suppressors of T-cell Receptor Signaling Sts-1 and Sts-2 Bind to Cbl and Inhibit Endocytosis of Receptor Tyrosine Kinases." J. Biol. Chem. 279(31): 32786-32795.

PubMed ID: 15159412 | Product(s) Referenced: Ub-Agarose

Chains

PubMed ID: 23345508 | Product(s) Referenced: Poly-Ub K11
PubMed ID: 23345508 | Product(s) Referenced: Poly-Ub K27
PubMed ID: 23345508 | Product(s) Referenced: Poly-Ub K29
PubMed ID: 23345508 | Product(s) Referenced: Poly-Ub K33
PubMed ID: 23345508 | Product(s) Referenced: Poly-Ub K48
PubMed ID: 23345508 | Product(s) Referenced: Poly-Ub K6
PubMed ID: 23345508 | Product(s) Referenced: Poly-Ub K63
PubMed ID: 23403031 | Product(s) Referenced: PYR-41
PubMed ID: 23682733 | Product(s) Referenced: K48-linked ubiquitin polymers (di-, tri-, tetra-, and penta-ubiquitin)
PubMed ID: 23746843 | Product(s) Referenced: DiUb of Lys27-,Lys29- and Lys33-linkages
PubMed ID: 23591816 | Product(s) Referenced: polyUb2-7 linked by Lys63
PubMed ID: 23708998 | Product(s) Referenced: diubiquitin substrate
PubMed ID: 23625928 | Product(s) Referenced: K48-linked ubiquitin chains
PubMed ID: 23625928 | Product(s) Referenced: K63-linked ubiquitin chains
PubMed ID: 23670163 | Product(s) Referenced: K48-linked polyubiquitin chains
PubMed ID: 23670163 | Product(s) Referenced: K63-linked polyubiquitin chains
PubMed ID: 23670163 | Product(s) Referenced: K63-linked polyubiquitin chains
PubMed ID: 23869922 | Product(s) Referenced: Lys-48-linked multi-ubiquitin chains
PubMed ID: 23869922 | Product(s) Referenced: Lys-63-linked multi-ubiquitin chains
PubMed ID: 24071738 | Product(s) Referenced: K48-linked polyubiquitin chains (Ub2-7)
PubMed ID: 24071738 | Product(s) Referenced: K63-linked polyubiquitin chains (Ub2-7)
PubMed ID: 24069405 | Product(s) Referenced: K63-linked ubiquitin

Inhibitors

PubMed ID: 23760478 | Product(s) Referenced: E1
PubMed ID: 23938298 | Product(s) Referenced: MG132
PubMed ID: 23454125 | Product(s) Referenced: MG132

UFM1

Bacik, J., et al. (2010) “Crystal Structure of the Human Ubiquitin-activating Enzyme 5 (UBA5) Bound to ATP: MECHANISTIC INSIGHTS INTO A MINIMALISTIC E1 ENZYME.” J. Biol. Chem. 285: 20273 - 20280.

PubMed ID: 20368332 | Product(s) Referenced: UFM1

FAT10

PubMed ID: 23452859 | Product(s) Referenced: recombinant FAT10