Product References

Boston Biochem's products have been used successfully in hundreds of UPP-related publications. We have listed our most recent references below to provide our customers with an exceptional resource for product use information. The references are organized to mirror our product divisions for easy location. Products referenced in each article are listed below in red. Please inquire to request archives as we have curated references back to 2003.

E2 Conjugating

Gazdoiu S., et al. (2005) "Proximity-induced activation of human Cdc34 through heterologous dimerization." PNAS 102(42): 15053-15058.

PubMed ID: 16210246 | Product(s) Referenced: APP-BP1, Uba3

Gentry M. S., et al. (2005) "From The Cover: Insights into Lafora disease: Malin is an E3 ubiquitin ligase that ubiquitinates and promotes the degradation of laforin." PNAS 102(24): 8501-8506.

PubMed ID: 15930137 | Product(s) Referenced: human E2

Horn P. J., et al. (2005) "A Rik1-associated, cullin-dependent E3 ubiquitin ligase is essential for heterochromatin formation." Genes & Dev. 19(14): 1705-1714.

PubMed ID: 6024659 | Product(s) Referenced: recombinant human UbcH5b

Jang I.-C., et al. (2005) "HFR1 is targeted by COP1 E3 ligase for post-translational proteolysis during phytochrome A signaling." Genes & Dev. 19(5): 593-602.

PubMed ID: 15741320 | Product(s) Referenced: human E2 UbcH5b

Kim D. W., et al. (2005) "The Shigella flexneri effector OspG interferes with innate immune responses by targeting ubiquitin-conjugating enzymes." PNAS 102(39): 14046-14051.

PubMed ID: | Product(s) Referenced: anti-UbcH5

Shirogane T., et al. (2005) "SCF[beta]-TRCP Controls Clock-dependent Transcription via Casein Kinase 1-dependent Degradation of the Mammalian Period-1 (Per1) Protein." J. Biol. Chem. 280(29): 26863-26872.

PubMed ID: 15917222 | Product(s) Referenced: Ubc5

Yan C., et al. (2005) "Activating transcription factor 3, a stress sensor, activates p53 by blocking its ubiquitination." EMBO J. 24(13): 2425-2435.

PubMed ID: 15933712 | Product(s) Referenced: E2

Chen L. and Madura K. (2005) "Increased Proteasome Activity, Ubiquitin-Conjugating Enzymes, and eEF1A Translation Factor Detected in Breast Cancer Tissue." Cancer Res. 65(13): 5599-5606.

PubMed ID: 15994932 | Product(s) Referenced: anti-Ubch1 (E2)

Fotia, A.B., et al. (2005). "The ubiquitin-protein ligases Nedd4 and Nedd4-2 show similar ubiquitin-conjugating enzyme specificities." Intl. J. Biochem. Cell. Biol. 38(3): 472-479.

PubMed ID: 16337426 | Product(s) Referenced: GST-UbcH5b, GST-UbcH5c, UbcH10, UbcH3, UbcH5b

Lois L. M. and Lima C. D. (2005) "Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1." EMBO J. 24(3): 439-451.

PubMed ID: 15660128 | Product(s) Referenced: Ubc12, Ubc5, Ubc7

Wang Z., et al. (2005) "RING-H2 Protein WSSV249 from White Spot Syndrome Virus Sequesters a Shrimp Ubiquitin-Conjugating Enzyme, PvUbc, for Viral Pathogenesis." J. Virol. 79(14): 8764-8772.

PubMed ID: 15994770 | Product(s) Referenced: human GST-UbcH5b / human UbcH1, human UbcH10, human UbcH2, human UbcH3, human UbcH5a, human UbcH9

Yogosawa S., et al. (2005) "Ubiquitylation and Degradation of Serum-inducible Kinase by hVPS18, a RING-H2 Type Ubiquitin Ligase." J. Biol. Chem. 280(50): 41619-41627.

PubMed ID: 16203730 | Product(s) Referenced: E2-enzyme mixture

Zhang D. D., et al. (2005) "Ubiquitination of Keap1, a BTB-Kelch Substrate Adaptor Protein for Cul3, Targets Keap1 for Degradation by a Proteasome-independent Pathway." J. Biol. Chem. 280(34): 30091-30099.

PubMed ID: 15983046 | Product(s) Referenced: E2-UbcH5a

Zhang Y., et al. (2005) "Recognition and Ubiquitination of Salmonella Type III Effector SopA by a Ubiquitin E3 Ligase, HsRMA1." J. Biol. Chem. 280(46): 38682-38688.

PubMed ID: 16176924 | Product(s) Referenced: UbcH5a

Adachi K., et al. (2004) "Ubiquitylation of Nascent Globin Chains in a Cell-free System." J. Biol. Chem. 279(40): 41767-41774.

PubMed ID: 15297454 | Product(s) Referenced: E2

Bartee E., et al. (2004) "Downregulation of Major Histocompatibility Complex Class I by Human Ubiquitin Ligases Related to Viral Immune Evasion Proteins." J. Virol. 78(3): 1109-1120.

PubMed ID: 14722266 | Product(s) Referenced: UbcH2

Fotia A. B., et al. (2004) "Regulation of Neuronal Voltage-gated Sodium Channels by the Ubiquitin-Protein Ligases Nedd4 and Nedd4-2." J. Biol. Chem. 279(28): 28930-28935.

PubMed ID: 15123669 | Product(s) Referenced: UbcH5b

Wei, W., et al.(2004) "Degradation of the SCF component Skp2 in cell-cycle phase G1 by the anaphase promoting complex." Nature 428: 194-198.

PubMed ID: 15014503 | Product(s) Referenced: UbCH-10

Yang Q.-H. and Du C. (2004) "Smac/DIABLO Selectively Reduces the Levels of c-IAP1 and c-IAP2 but Not That of XIAP and Livin in HeLa Cells." J. Biol. Chem. 279(17): 16963-16970.

PubMed ID: 14960576 | Product(s) Referenced: various recombinant human E2

Burstein E., et al. (2004) "A novel role for XIAP in copper homeostasis through regulation of MURR1." EMBO J. 23(1): 244-254.

PubMed ID: 14685266 | Product(s) Referenced: E2

Mudgil Y., et al. (2004) "A Large Complement of the Predicted Arabidopsis ARM Repeat Proteins Are Members of the U-Box E3 Ubiquitin Ligase Family." Plant Physiology 134(1): 59-66.

PubMed ID: 14657406 | Product(s) Referenced: human UBC10, human UBC2A, human UBC3, human UBC5A, human UBC5B, human UBC6, human UBC7

Oh K.-J., et al. (2004) "The Papillomavirus E7 Oncoprotein Is Ubiquitinated by UbcH7 and Cullin 1- and Skp2-Containing E3 Ligase." J. Virol. 78(10): 5338-5346.

PubMed ID: 15113913 | Product(s) Referenced: ubiquitin-conjugating enzyme E2

Peng H.-M., et al. (2004) "Ubiquitylation of Neuronal Nitric-oxide Synthase by CHIP, a Chaperone-dependent E3 Ligase." J. Biol. Chem. 279(51): 52970-52977.

PubMed ID: 15466472 | Product(s) Referenced: GST-tagged UbcH5a

Plafker S. M., et al. (2004) "Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import." J. Cell Biol. 167(4): 649-659.

PubMed ID: 15545318 | Product(s) Referenced: anti-UbcH6

Secombe J. and Parkhurst S.M. (2004) "Drosophila Topors Is a RING Finger-containing Protein That Functions as a Ubiquitin-protein Isopeptide Ligase for the Hairy Basic Helix-Loop-Helix Repressor Protein." J. Biol. Chem. 279(17): 17126-17133.

PubMed ID: 14871887 | Product(s) Referenced: human UbcH5a

E3 Ligase

PubMed ID: 24210823 | Product(s) Referenced: E3 kit
PubMed ID: 24124579 | Product(s) Referenced: GST-MDM2
PubMed ID: 23512198 | Product(s) Referenced: his-purified recombinant Cullin 1
PubMed ID: 23512198 | Product(s) Referenced: his-purified recombinant Rbx1
PubMed ID: 23512198 | Product(s) Referenced: his-purified recombinant Skp1
PubMed ID: 23785288 | Product(s) Referenced: E3 ligase

Johnson, B.N. et al. (2012) "The ubiquitin E3 ligase parkin regulates the proapoptotic function of Bax." Proc. Natl. Acad. Sci. U.S.A. 109(16): 6283–6288.

PubMed ID: 22460798

Burchell, L. et al. (2012) "Small, N-terminal tags activate parkin e3 ubiquitin ligase activity by disrupting its autoinhibited conformation." PLoS ONE 7(4): e34748.

PubMed ID: 22496854 | Product(s) Referenced: His-Parkin

Johnson, B.N. et al. (2012) "The ubiquitin E3 ligase parkin regulates the proapoptotic function of Bax." Proc. Natl. Acad. Sci. U.S.A. 109(16): 6283–6288.

PubMed ID: 22460798 | Product(s) Referenced: UbcH7

Schneider, S. et al. (2012) "Development of a homogeneous AlphaLISA ubiquitination assay using ubiquitin binding matrices as universal components for the detection of ubiquitinated proteins." Biochimica et biophysica acta. 2012 Apr 5.

PubMed ID: 22504171

Hou, X. et al. (2012) "Mining and characterization of ubiquitin E3 ligases expressed in the mouse testis." BMC Genomics 13: 495.

PubMed ID: 22992278 | Product(s) Referenced: MYC-ubiquitin, purified recombinant E2 (UBE2D2/UBCH5B), purified recombinant E2 (UBE2D3/UBCH5C), purified recombinant E2 (Ube2k/E2-25K), purified recombinant E2 (UBE2L3/ UBCH7), putative E3s with GST tag, yeast E1