Product References - E2 conjugating

Boston Biochem's products have been used successfully in hundreds of UPP-related publications. We have listed our most recent references below to provide our customers with an exceptional resource for product use information. The references are organized to mirror our product divisions for easy location. Products referenced in each article are listed below in red. Please inquire to request archives as we have curated references back to 2003.

Hebron, M.L. et al. (2012) "Parkin ubiquitinates Tar-DNA binding protein-43 (TDP-43) and promotes its cytosolic accumulation via interaction with histone deacetylase 6 (HDAC6)." J. Biol. Chem.

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23258539 | recombinant human ubiquitin, recombinant UbcH7, E1 recombinant enzyme,
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23328397 | UbcH5c
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23453969 | Ubc13 E2 enzyme
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23455478 | rabbit E1 (UBE1)
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23455478 | rabbit E2 (UbcH5b)
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23459942 | UbcH5b
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23287719 | E2 (Ubch7)
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23401859 | GST-UBC12
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23332158 | “Ubiquitination assays were performed using enzymes obtained from Boston Biochem”
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23362280 | UbcH5b
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23555887 | E2
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23463011 | Rad6B
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23486064 | Ubch3
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23486064 | Ubch5c
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23269275 | E2 (Ubc5a)
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23150437 | E2 enzyme (UbcH5a)
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23150407 | recombinant human E2 enzyme UbcH5c
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23314748 | UbcH5
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23418353 | UbcH13/Uev1a
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23418353 | UbcH5b
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23453970 | recombinant human UbcH5a/UBE2D1
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23453805 | hRAD6B
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23453805 | hUbcH13
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23453805 | hUbcH5c
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23212909 | UbcH5b
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23212909 | UbcH5c
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23452855 | E2 UbcH5c
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23452855 | http://www.ncbi.nlm.nih.gov/pubmed/?term=23452855 His6-Ubc13/His6-Uev1A
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23512198 | Ubc7
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23328397 | UbcH10

Zhang, S. et al. (2012) "GST:Identification of RNF8 as a ubiquitin ligase involved in targeting the p12 subunit of DNA polymerase δ for degradation in response to DNA damage." J. Biol. Chem.

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23233665 | ATP regenerating solution (ENS), ubiquitin aldehyde, HeLa S100 fraction, Recombinant human E1 ubiquitin activating enzyme, Recombinant human ubiquitin, Recombinant human ubiquitin K48R, Recombinant human ubiquitin K63R, Recombinant human UbcH5c, Recombinant human Cdc34, Recombinant human UbcH2

Liu, C. et al. (2012) "CHFR is important for the first wave of ubiquitination at DNA damage sites." Nucleic Acids Res.

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23268447 | HA-Ub, E1, UbcH5C, Ubc13/Uev1a

Gerard, B. et al. (2012) "Lysine 394 is a novel Rad6B-induced ubiquitination site on beta-catenin." Biochimica et biophysica acta.

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22705350 | catalytically inactive rad6B, wild-type Rad6B

Guo, Z. et al. (2012) "Sequential Posttranslational Modifications Program FEN1 Degradation during Cell-Cycle Progression." Molecular cell.

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22749529 | ubiquitination reaction buffer, UBE1, UBE2M, HeLa S100 fraction, SUMOylation kits, ubiquitination kit

Cohen, S. et al. (2012) "Ubiquitylation by Trim32 causes coupled loss of desmin, Z-bands, and thin filaments in muscle atrophy." J. Cell Biol. 198(4): 575–589.

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22908310 | UbcH13, Uev1 E2-conjugating enzyme

Chung, D.-W.D. et al. (2012) "Characterization of the Ubiquitylating Components of the Human Malaria Parasite’s Protein Degradation Pathway." PLoS ONE 7(8): e43477.

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22912882 | re-energizing buffer, human recombinant UBE1 E1, Suc-LLVY-AMC, human recombinant UBC E2, biotin conjugated ubiquitin, ubiquitin,

Johnson, A.E. et al. (2012) "Fission Yeast Dma1 Requires RING Domain Dimerization for Its Ubiquitin Ligase Activity and Mitotic Checkpoint Function." J. Biol. Chem.

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10.1074/jbc.M112.349712 | recombinant E1, Ubc13/Uev1 E2 complex,

Janzer, A. et al. (2012) "The H3K4me3 histone demethylase Fbxl10 is a regulator of chemokine expression, cellular morphology and the metabolome of fibroblasts." The Journal of biological chemistry.

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22825849 | GST-UBC5a  human  recombinant protein

Hoxhaj, G. et al. (2012) "ZNRF2 is released from membranes by growth factors and with ZNRF1 regulates the Na+/K+ATPase." Journal of cell science.

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22797923 | E1 ubiquitin ligase, Ubc13/UEV1a, UbcH5a, ubiquitin

Zhuang, M. et al. (2012) "Substrates of IAP Ubiquitin Ligases Identified with a Designed Orthogonal E3 Ligase, the NEDDylator." Mol. Cell.

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23201124 | UbcH5b, UBE1, ubiquitin

Zhong, H.-J. et al. (2012) "A Metal-Based Inhibitor of NEDD8-Activating Enzyme." PLoS ONE 7(11): e49574.

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23185368 | anti-Ubc12 rabbit polyclonal antibody, NEDD8 Conjugation Initiation Kit

Zhao, Y. et al. (2012) "Non-Covalent Interaction between Polyubiquitin and GTP Cyclohydrolase 1 Dictates Its Degradation." PLoS ONE 7(9): e43306.

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22984419 | mixed ubiquitin chains (Ub2~7), sample buffer, Tetraubiquitin (Ub4), UIM-agarose

Zhang, Z. et al. (2012 "1,10-Phenanthroline promotes copper complexes into tumor cells and induces apoptosis by inhibiting the proteasome activity." J. Biol. Inorg. Chem. 17(8): 1257–1267.

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23053530 | purified human 20S proteasome, human ubiquitin activating enzyme E1, UbcH10

Zang, Y. et al. (2012) "Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage." Rice 5(1): 24.

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10.1186/1939-8433-5-24 | E1 enzyme, K48R ubiquitin, K63R ubiquitin, reaction buffer, Ub, Ubc13, Uev1A, ubiquitin-AMC, nonbiotinylated USP2 catalytic domain, USP10, USP47, USP5, USP7

Wang, J. et al. (2012) "A subset of mixed lineage leukemia proteins has PHD domain-mediated E3 ligase activity." J. Biol. Chem.

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23129768 | CDC34 antibody, HA tagged ubiquitin, human recombinant CDC34, human recombinant RAD6, human recombinant UBCH5c, human recombinant UBE1, recombinant histone H2A, recombinant histone H2B, recombinant histone H3, recombinant histone H4

Shibata, Y. et al. (2012) "p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO." Nat Commun 3: 1061.

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22990857 | His6-UbcH13/Uev1a, His6-UBE1, K48 only ubiquitin, K63 only ubiquitin, Lys48-linked polyubiquitin chains, Lys63-linked polyubiquitin chains, UbcH5c, wild-type ubiquitin

Shen, R.R. et al. (2012) "IKK{epsilon} phosphorylates TRAF2 to promote mammary epithelial cell transformation." Mol. Cell. Biol. 32(23):4756-68. 

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23007157 | Recombinant E1 ubiquitin activating enzyme, Ubc13 E2 enzyme, ubiquitin

Perrotta, I. et al. (2012) "Immunohistochemical analysis of the ubiquitin-conjugating enzyme UbcH10 in lung cancer: a useful tool for diagnosis and therapy." J. Histochem. Cytochem. 60(5): 359–365.

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22388643 | rabbit polyclonal anti-α-UbcH10

Pallante, P. et al. (2012) "UbcH10 overexpression in human lung carcinomas and its correlation with EGFR and p53 mutational status." Eur. J. Cancer.

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23102841 | anti-UbcH10

Neilsen, P.M. et al. (2012) "New 26S Proteasome Inhibitors with High Selectivity for Chymotrypsin-Like Activity and p53-Dependent Cytotoxicity." ACS Chem. Biol.

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23190346 | purified rabbit20S proteasome, Suc-LLVY-AMC

Mössinger, J. et al. (2012) "Phosphatidylinositol 4-kinase IIα function at endosomes is regulated by the ubiquitin ligase Itch." EMBO Rep. 13(12):1087-94.

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23146885 | E1 (UBE1), E2 (UbcH7)

Lu, C.-S., Truong, L.N., et al. (2012) "The RING finger protein RNF8 ubiquitinates Nbs1 to promote DNA double-strand break repair by homologous recombination." J. Biol. Chem.

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23115235 | rabbit E1, UbcH5c, E1, E2, ubiquitin

Jung, Y.-S. et al. (2012) "Pirh2 E3 Ubiquitin Ligase Modulates Keratinocyte Differentiation through p63." J. Invest. Dermatol.

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23235527 | E1, E2, ubiquitin

Liu, Z. et al. (2012) "Enhancement of Ubiquitin Conjugation Activity Reduces Intracellular Aggregation of V76D Mutant γD-Crystallin." Invest. Ophthalmol. Vis. Sci. 53(10): 6655–6665.

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22915036 | rabbit polyclonal antibody to UbcH5

Humphrey, R.K. et al. (2012) "Lysine 63-linked Ubiquitination Modulates Mixed Lineage Kinase-3 Interaction with JIP1 Scaffold protein in Cytokine-induced Pancreatic Beta Cell Death." J. Biol. Chem.

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23172226 | Mg-ATP mix cofactor mix, Myc-ubiquitin, recombinant UbE1,UbeH13-Uev1a heterodimer complex, Ub conjugation buffer,

Ling, Q. et al. (2012) "Chloroplast biogenesis is regulated by direct action of the ubiquitin-proteasome system." Science 338(6107): 655–659.

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23118188 | 6×His-tagged AtUBC8 E2, HA-ubiquitin

Lee, T.-H. et al. (2012) "Coordinated regulation of XPA stability by ATR and HERC2 during nucleotide excision repair." Oncogene.

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23178497 | GST-UbcH5a, HA-ubiquitin, recombinant UBE1

Hou, X. et al. (2012) "Mining and characterization of ubiquitin E3 ligases expressed in the mouse testis." BMC Genomics 13: 495.

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22992278 | MYC-ubiquitin, purified recombinant E2 (UBE2D2/UBCH5B), purified recombinant E2 (UBE2D3/UBCH5C), purified recombinant E2 (Ube2k/E2-25K), purified recombinant E2 (UBE2L3/ UBCH7), putative E3s with GST tag, yeast E1

Chau, J.F.L. et al. (2012) "A crucial role for bone morphogenetic protein-Smad1 signalling in the DNA damage response." Nat Commun 3: 836.

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22588298 | E2 (UbcH5c-gst), E1 (UBE1-gst),

Burrows, A.C. et al. (2012) "Skp1-Cul1-F-box Ubiquitin Ligase (SCFβTrCP)-Mediated Destruction of the Ubiquitin-Specific Protease USP37 During G2-phase Promotes Mitotic Entry." J. Biol. Chem.

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23027877 | MG132, UbcH10 antibody

Avila, J. and Devarenne, T.P. (2012) "Ubiquitination of the tomato cell death suppressor Adi3 by the RING E3 Ubiquitin Ligase AdBiL." Biochem. Biophys. Res. Commun.

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23178567 | FLAG tagged ubiquitin, Human E2s from the UbcH Enzyme Set

Hernandez-Ortega, S. et al. (2012) "Defective in Mitotic Arrest 1 (Dma1) ubiquitin ligase controls G1 cyclin degradation." J. Biol. Chem.

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23264631 | E1 enzyme (Uba1), E2 complex (HIS6x-Ubc13, HIS6x-Mms2),

Bai, L. et al. (2012) "UHRF2, another E3 ubiquitin ligase for p53." Biochemical and Biophysical Research Communications(0).

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22902639 | E1, E2 (UbcH5a)

Guo, H. et al. (2012) "E3 Ubiquitin Ligase Cbl-b Regulates Pten via Nedd4 in T Cells Independently of Its Ubiquitin Ligase Activity." Cell Reports 1: 472–482.

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10.1016/j.celrep.2012.04.008 | Ubc H5

Guo, H. et al. (2012) "E3 Ubiquitin Ligase Cbl-b Regulates Pten via Nedd4 in T Cells Independently of Its Ubiquitin Ligase Activity." Cell Reports 1: 472–482.

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10.1016/j.celrep.2012.04.008 | Ubc5

Liu, Y. et al. (2012) "NF-κB Repression by PIAS3 Mediated RelA SUMOylation." PLoS ONE 7(5): e37636.

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22649547 | Ubc9

Xin, D.-W. et al. (2012) "Functional Analysis of NopM, a Novel E3 Ubiquitin Ligase (NEL) Domain Effector of Rhizobium sp. Strain NGR234." PLoS Pathog. 8(5): e1002707.

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22615567 | UbcH5B (E2)

Milhollen, M.A. et al. (2012) "Treatment-emergent mutations in NAEβ confer resistance to the NEDD8-activating enzyme inhibitor MLN4924." Cancer Cell 21(3): 388–401.

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22439935 | UBC10

Luo, K. et al. (2012) "Sumoylation of MDC1 is important for proper DNA damage response." The EMBO journal.  2012 May 25.

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22635276 | E2

Chen, J. et al. (2012) "The ubiquitin ligase mLin41 temporally promotes neural progenitor cell maintenance through FGF signaling." Genes Dev. 26(8): 803–815.

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22508726 | Ubch5a

Gontan, C. et al. (2012) "RNF12 initiates X-chromosome inactivation by targeting REX1 for degradation." Nature 485(7398): 386–390.

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22596162 | UBCH5A

Luo, K. et al. (2012) "Sumoylation of MDC1 is important for proper DNA damage response." The EMBO journal.  2012 May 25;

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22635276 | UbcH5a

Pulvino, M. et al. (2012) "Inhibition of proliferation and survival of diffuse large B-cell lymphoma cells by a small-molecule inhibitor of the ubiquitin-conjugating enzyme Ubc13-Uev1A." Blood.

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22791293 | anti-UbcH5

Hu, R. et al. (2012) "Distinct transcriptional programs mediated by the ligand-dependent full-length androgen receptor and its splice variants in castration-resistant prostate cancer." Cancer research.

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22710436 | anti-UBE2C

Maezawa, S. et al. (2012) "Ubiquitylation of terminal deoxynucleotidyltransferase inhibits its activity." PLoS ONE 7(7): e39511.

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22808041 | rabbit pAb against UbcH5, rabbit pAb against UbcH6, rabbit pAb against UbcH7

An, J.Y. et al. (2012) "UBR2 of the N-End Rule Pathway Is Required for Chromosome Stability via Histone Ubiquitylation in Spermatocytes and Somatic Cells." PLoS ONE 7(5): e37414.

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22616001 | HR6B

Wu, J. et al. (2012) "Skp2 E3 Ligase Integrates ATM Activation and Homologous Recombination Repair by Ubiquitinating NBS1." Molecular cell. 2012 Mar 28;

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22464731 | E1, UBCH5c

Osula, O. et al. (2012) "Identification of protein SUMOylation sites by mass spectrometry using combined microwave-assisted aspartic acid cleavage and tryptic digestion." J Mass Spectrom 47(5): 644–654.

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22576878 | E2-25K substrate

Sambuughin, N. et al. (2012) "KBTBD13 interacts with Cullin 3 to form a functional ubiquitin ligase." Biochemical and biophysical research communications. 2012 Apr 20;

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22542517 | E2-UbcH5a

Shabbeer, S. et al. (2012) "BRCA1 targets G2/M cell cycle proteins for ubiquitination and proteasomal degradation." Oncogene.

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23246971 | recombinant His-cyclin B, HA-ubiquitin, E1, UbcH5c, UbcH1, Ubc13-MMS2, UbcH10, ATP (ERS), His-BRCA1, His-BARD1

Durcan, T.M., et al. (2011) “The Machado–Joseph disease-associated mutant form of ataxin-3 regulates parkin ubiquitination and stability.” Human Molecular Genetics, 20: 141-154.

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20940148 | E2

Gannavaram, S., et al. (2011) “Mitochondrial Associated Ubiquitin Fold Modifier-1 Mediated Protein Conjugation in Leishmania donovani.” PLoS one 6: e16156. doi:10.1371/journal.pone.0016156.

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21264253 | Human recombinant Uba5

Hodson, C., et al. (2011) “Structural Analysis of Human FANCL, the E3 Ligase in the Fanconi Anemia Pathway.” J. Biol. Chem. 286: 32628 - 32637.

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21775430 | E2

Zhu, F., et al. (2011) “Phosphorylation of H2AX at Ser139 and a New Phosphorylation Site Ser16 by RSK2 Decreases H2AX Ubiquitination and Inhibits Cell Transformation.” Cancer Research, 71: 393.

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21224359 | recombinant UbcH5a

Cao, X., et al. (2011) “WW Domain-containing E3 Ubiquitin Protein Ligase 1 (WWP1) Delays Cellular Senescence by Promoting p27 Kip1 Degradation in Human Diploid Fibroblasts.” J. Biol. Chem. 286: 33447 - 33456.

21795702 | UbcH7

Cotto-Rios, X.M., et al. (2011) “APC/CCdh1-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage.” J. Cell Biol. 194: 177 - 186.

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21768287 | UBCH10, UBCH5

Galisson, F., et al. (2011) “A Novel Proteomics Approach to Identify SUMOylated Proteins and Their Modification Sites in Human Cells.” Molecular and Cellular Proteomics, 10: M110.004796.

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21098080 | hUbC9, GST- RanGAP fragment 418–587

Hadian, K., et al. (2011) “NF-B Essential Modulator (NEMO) Interaction with Linear and Lys-63 Ubiquitin Chains Contributes to NF-B Activation.” J. Biol. Chem. 286: 26107 - 26117.

21622571 | Lys-63 specific E2 complex UbcH13/Uev1a

Huang, Z., et al. (2011) “Deubiquitylase HAUSP stabilizes REST and promotes maintenance of neural progenitor cells.” Nature Cell Biology, 13: 142-152.

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21258371 | His–Ubc H3, His–Ubc H5

Jones, J., et al. (2011) “The RAG1 V(D)J recombinase/ubiquitin ligase promotes ubiquitylation of acetylated, phosphorylated histone 3.3.” Immunology Letters, 2: 156-162.

1256161 | UbcH2

Kim, J., et al (2011) “Role of TRIM5[alpha] RING Domain E3 Ubiquitin Ligase Activity in Capsid Disassembly, Reverse Transcription Blockade, and Restriction of Simian Immunodeficiency Virus.” J. Virol. 85: 8116 - 8132.

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21680520 | UbcH5a

Lin, D., et al. (2011) “Biochemical and Structural Studies of a HECT-like Ubiquitin Ligase from Escherichia coli O157:H7.” The Journal of Biological Chemistry, 286: 441-449.

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20980253 | E2 enzymes

Lu, J., et al. (2011) “RNF170 Protein, an Endoplasmic Reticulum Membrane Ubiquitin Ligase, Mediates Inositol 1,4,5-Trisphosphate Receptor Ubiquitination and Degradation.” J. Biol. Chem. 286: 24426 - 24433.

21610068 | UbcH5b

Lu, K., et al. (2011) “Pivotal role of the SMURF1 C2 domain in substrate selection.” The Journal of Biological Chemistry: doi:10.1074/jbc.M110.211979.

21402695 | UbcH5c (E2)

Mao, X., et al. (2011) “COMMD1 (Copper Metabolism MURR1 Domain-containing Protein 1) Regulates Cullin RING Ligases by Preventing CAND1 (Cullin-associated Nedd8-dissociated Protein 1) Binding.” J. Biol. Chem. 286: 32355 - 32365.

21778237 | human His6-E1 (Uba1), UbcH10, UbcH3, UbcH5a, UbcH5b, UbcH5c, UbcH7

Meisenberg, C., et al. (2011) “Ubiquitin ligase UBR3 regulates cellular levels of the essential DNA repair protein APE1 and is required for genome stability.” Nucleic Acids Res. 10.1093/nar/gkr744.

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21933813 | E2

Nagel, C., et al. (2011) “Herpes Simplex Virus Immediate-Early Protein ICP0 Is Targeted by SIAH-1 for Proteasomal Degradation.” J. Virol. 85: 7644 - 7657.

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21632771 | ubiquitin-conjugating enzyme UbcH5b

Shibata, E., et al. (2011) “Selective Ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G Ubiquitin-Conjugating Enzymes via the CRL4Cdt2 Ubiquitin Ligase Complex.” Mol. Cell. Biol. 31: 3136 - 3145.

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21628527 | recombinant UBCH5C

Sung, C.K., et al. (2011) “Transcriptional and post-translational regulation of the quiescence factor and putative tumor suppressor p150Sal2.” The FASEB Journal fj.10-173674; published ahead of print January 12, 2011,doi:10.1096/fj.10-173674.

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21228219 | UbcH5c (E2-627)

Wang, Y., et al. (2011) “The ubiquitin-dependent proteasomal degradation (UPD) of human liver cytochrome P450 2E1: identification of sites targeted for phosphorylation and Ubiquitination.” The Journal of Biological Chemistry: doi: 10.1074/jbc.M110.176685 .

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21209460 | UbcH5a

Wu, K., et al. (2011) “Mono-ubiquitination Drives Nuclear Export of the Human DCN1-like Protein hDCNL1.” J. Biol. Chem. 286: 34060 - 34070

21813641 | APP-BP, Uba3, Ubc12, UbcH5c

Zhang, N., et al. (2011) “Ubiquitin Chain Trimming Recycles the Substrate Binding Sites of the 26 S Proteasome and Promotes Degradation of Lysine 48-linked Polyubiquitin Conjugates.” J. Biol. Chem. 286: 25540 - 25546.

21632534 | UbcH10

Zhao, R., et al. (2011) “Subunit 6 of the COP9 signalosome promotes tumorigenesis in mice through stabilization of MDM2 and is upregulated in human cancers.” JCI, 121: 851-865.

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21317535 | E2 (E2-622)

Cui, Y., et al. (2011) "SCFFBXL15 regulates BMP signalling by directing the degradation of HECT-type ubiquitin ligase Smurf1.” The EMBO Journal 30(13): 2675–2689.

21572392 | UbcH5c

Fischer, E.S., et al. (2011) "The Molecular Basis of CRL4DDB2/CSA Ubiquitin Ligase Architecture, Targeting, and Activation.” Cell 147(5): 1024–1039.

22118460 | hsUbcH5A, hsUbcH5B

Hessa, T., et al. (2011) "Protein targeting and degradation are coupled for elimination of mislocalized proteins.” Nature 475(7356): 394–397.

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21743475 | all mammalian E2 enzymes

Huang, H.S., et al. (2011) "Topoisomerase inhibitors unsilence the dormant allele of Ube3a in neurons.” Nature 481(7380): 185–189.

22190039 | UBCH7

Ikeda, F., et al. (2011) "SHARPIN forms a linear ubiquitin ligase complex regulating NF-[kappa] B activity and apoptosis.” Nature 471(7340): 637–641.

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21455181 | UbcH7

Kassmeier, M.D., et al. (2011) "VprBP binds full-length RAG1 and is required for B-cell development and V (D) J recombination fidelity.” The EMBO Journal. 31(4):945-58.

22157821 | UbcH13, UbcH2, UbcH3, UbcH5a, UbcH5b

Kho, C., et al. (2011) "SUMO1-dependent modulation of SERCA2a in heart failure.” Nature 477(7366): 601–605.

21900893 | UBC9

Maddika, S., et al. (2011) "WWP2 is an E3 ubiquitin ligase for PTEN.” Nature cell biology 13(6): 730–735.

21750408 | UbcH5b

Oberoi, T.K., et al. (2011) "IAPs regulate the plasticity of cell migration by directly targeting Rac1 for degradation.” The EMBO Journal 31(1): 14–28.

22117219 | UbcH5a

Solé, C., et al. (2011) "Control of Ubp3 ubiquitin protease activity by the Hog1 SAPK modulates transcription upon osmostress.” The EMBO Journal 30(16): 3274–3284.

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21743437 | Ubc5H5c

Song, M.S., et al. (2011) "Nuclear PTEN Regulates the APC-CDH1 Tumor-Suppressive Complex in a Phosphatase-Independent Manner.” Cell 144(2): 187–199.

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21241890 | UbcH10, Ubc5b

Tang, L.Y., et al. (2011) "Ablation of Smurf2 reveals an inhibition in TGF-[beta] signalling through multiple mono-ubiquitination of Smad3.” The EMBO Journal. 30(23):4777-89.

22045334 | E2-conjugating enzyme UbcH5c

Wang, F., et al. (2011) "Deacetylation of FOXO3 by SIRT1 or SIRT2 leads to Skp2-mediated FOXO3 ubiquitination and degradation.” Oncogene. 31(12):1546-57.

21841822 | UbcH3, Cdc34

Zhang, X., et al. (2011) "USP4 inhibits p53 through deubiquitinating and stabilizing ARF-BP1.” The EMBO Journal 30(11): 2177–2189.

21522127 | E2 (His-UbcH5a)

Zhang, Y., et al. (2011) "RNF146 is a poly (ADP-ribose)-directed E3 ligase that regulates axin degradation and Wnt signalling.” Nature Cell Biology 13(5): 623–629.

21478859 | E2 (UbcH5a)

Maddika, S., et al. (2011) "WWP2 is an E3 ubiquitin ligase for PTEN.” Nature cell biology 13(6): 730–735.

21750408 | UbcH5b

Bacik, J., et al. (2010) “Crystal Structure of the Human Ubiquitin-activating Enzyme 5 (UBA5) Bound to ATP: MECHANISTIC INSIGHTS INTO A MINIMALISTIC E1 ENZYME.” J. Biol. Chem. 285: 20273 - 20280.

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20368332 | UFC1

Banerjee, S., et al. (2010) “Modulation of SCF[beta]-TrCP-dependent IB Ubiquitination by Hydrogen Peroxide.” J. Biol. Chem. 285: 2665 - 2675.

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19933270 | His-tagged UbcH3

Dolan, P.J. and Johnson, G. (2010) “A Caspase Cleaved Form of Tau Is Preferentially Degraded through the Autophagy Pathway.” J. Biol. Chem. 285: 21978 - 21987.

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20466727 | Recombinant E1, UbcH5b

Ensminger, A. and Isberg, R. (2010) “E3 ubiquitin ligase activity and targeting of BAT3 by multiple Legionella pneumophila translocated substrates.” Infect. Immun., 78: 3905 - 3919.

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20547746 | E2

Hallows, K.R., et al. (2010) “Phosphopeptide Screen Uncovers Novel Phosphorylation Sites of Nedd4-2 That Potentiate Its Inhibition of the Epithelial Na+ Channel.” J. Biol. Chem. 285: 21671 - 21678.

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20466724 | rabbit E2

Kaneko, M., et al. (2010) “Loss of HRD1-Mediated Protein Degradation Causes Amyloid Precursor Protein Accumulation and Amyloid-[beta] Generation.” J. Neurosci., 3924 - 3932.

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20237263 | GST-UbcH5c (E2)

Kang, T.-H., et al. (2010) “Regulation of nucleotide excision repair activity by transcriptional and post-transcriptional control of the XPA protein.” Nucleic Acids Res. 10.1093/nar/gkq1318.* .

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21193487 | GST-UbcH5a

Ahlskog, J. K., et al. (2010) “Anaphase-Promoting Complex/Cyclosome Participates in the Acute Response to Protein-Damaging Stress.” Mol. Cell. Biol. 30: 5608-5620.

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20937767 | UbcH10

An, J.Y., et al. (2010) “UBR2 mediates transcriptional silencing during spermatogenesis via histone ubiquitination.” PNAS 107: 1912 - 1917.

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20080676 | GST-HR6B, HR6B

Li, X.S., et al. (2010) "Mammalian SWI/SNF-A Subunit BAF250/ARID1 Is an E3 Ubiquitin Ligase That Targets Histone H2B." Mol. Cell. Biol. 30: 1673 - 1688.

Full Paper
20086098 | E2 (UbcH5c)

Luo, H., et al. (2010) “The Arabidopsis Botrytis Susceptible1 Interactor Defines a Subclass of RING E3 Ligases That Regulate Pathogen and Stress Responses.” Plant Physiology 154: 1766-1782.

Full Paper
20921156 | E2 UbcH5b

Mo, P., et al. (2010) “MDM2 Mediates Ubiquitination and Degradation of Activating Transcription Factor 3.” J. Biol. Chem. 285: 26908 - 26915.

Full Paper
20592017 | E2

Patel, D. and McCance, D. J. (2010) “Compromised Spindle Assembly Checkpoint due to Altered Expression of Ubch10 and Cdc20 in Human Papillomavirus Type 16 E6- and E7-Expressing Keratinocytes.” J. Virol. 84: 10956-10964.

| Ubch10

Ramamoorthy, S., et al. (2010) “Isoform-Specific Degradation of PR-B by E6-AP Is Critical for Normal Mammary Gland Development.” Mol. Endocrinol. 24: 2099-2113.

Full Paper
20829392 | E2

Walters, M.S., et al. (2010) “The RING Finger Domain of Varicella-Zoster Virus ORF61p Has E3 Ubiquitin Ligase Activity That Is Essential for Efficient Autoubiquitination and Dispersion of Sp100-Containing Nuclear Bodies.” J. Virol. 84: 6861 - 6865.

Full Paper
20392849 | His-UbcH3, UbcH5c

Wu, T., et al. (2010) “UBE2S drives elongation of K11-linked ubiquitin chains by the Anaphase-Promoting Complex.” PNAS 107: 1355 - 1360.

Full Paper
20080579 | UbcH5a

Yang, Y., et al. (2010) “E3 ubiquitin ligase Mule ubiquitinates Miz1 and is required for TNF-induced JNK activation.” PNAS 107: 13444 - 13449.

Full Paper
20624960 | recombinant E2 (UbcH5)

Alvarez, S.E., et al. (2010) "Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2.” Nature 465(7301): 1084–1088.

Full Paper
20577214 | UbcH13–Uev1a E2 complex, UbcH5–Uev1a E2 complex

Chu, Y. and Yang, X. (2010) "SUMO E3 ligase activity of TRIM proteins.” Oncogene 30(9): 1108–1116.

Full Paper
20972456 | Ubc9

Chymkowitch, P., et al. (2010) "The phosphorylation of the androgen receptor by TFIIH directs the ubiquitin/proteasome process.” The EMBO Journal 30(3): 468–479.

Full Paper
21157430 | E2 (GST-UbcH5a)

Dynek, J.N., et al. (2010) "c-IAP1 and UbcH5 promote K11-linked polyubiquitination of RIP1 in TNF signalling.” The EMBO journal 29(24): 4198–4209.

Full Paper
21113135 | UbcH5a, UbcH5b, UbcH5c

Johnson, A.E. and Gould, K.L. (2010) "Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase.” The EMBO journal 30(2): 341–354.

Full Paper
21131906 | E2 (Ubc13/Uev1a)

Cohen, S., et al. (2009) "During muscle atrophy, thick, but not thin, filament components are degraded by MuRF1-dependent ubiquitylation." J. Cell Biol. 185: 1083-1095.

Full Paper
19506036 | UbcH1 E2-conjugating enzyme

Corcoran, C., et al. (2009) “Identification and Characterization of Two Novel Isoforms of Pirh2 Ubiquitin Ligase That Negatively Regulate p53 Independent of RING Finger Domains.” J. Biol. Chem. 284: 21955 – 21970 .

Full Paper
19483087

Enzler, T., et al. (2009) “MEKK1 Binds HECT E3 Ligase Itch by Its Amino-Terminal RING Motif to Regulate Th2 Cytokine Gene Expression.” J. Immunol. 183: 3831 - 3838.

Full Paper
19710465 | ubiquitination assay enzymes

Faustrup, H., et al. (2009) "USP7 counteracts SCF[beta]TrCP- but not APCCdh1-mediated proteolysis of Claspin." J. Cell Biol. 184(1):13-19.

Full Paper
19124652 | hUbcH5C

Fu, W., et al. (2009) "MDM2 Acts Downstream of p53 as an E3 Ligase to Promote FOXO Ubiquitination and Degradation." J. Biol. Chem. 284: 13987-14000.

Full Paper
19321440 | GST-Ubc5Hb

Isobe, T., et al. (2009) “Adenovirus E1A Inhibits SCFFbw7 Ubiquitin Ligase.” J. Biol. Chem. 284: 27766 - 27779.

Full Paper
19679664 | UbcH5c

Kadara, H., et al. (2009) “Identification of Gene Signatures and Molecular Markers for Human Lung Cancer Prognosis using an In vitro Lung Carcinogenesis System.” Cancer Prevention Research 2: 702 - 711.

Full Paper
19638491

Merbl, Y. and Kirschner M.W. (2009) "Large-scale detection of ubiquitination substrates using cell extracts and protein microarrays." PNAS 10 (8): 2543-2548.

Full Paper
19181856 | UbcH10

Sigl, R., et al. (2009) “Loss of the mammalian APC/C activator FZR1 shortens G1 and lengthens S phase but has little effect on exit from mitosis.” J. Cell Sci. 122: 4208 - 4217.

Full Paper
19861496 | UbcH10 (rb)

Bernal-Bayard, J. and Ramos-Morales, F. (2009) “Salmonella Type III Secretion Effector SlrP Is an E3 Ubiquitin Ligase for Mammalian Thioredoxin.” J. Biol. Chem. 284: 27587 - 27595.

Full Paper
19690162 | human recombinant UbcH5b

Burns, M., et al. (2009) "Parkin promotes intracellular A[beta]1–42 clearance." Hum. Mol. Genet. 18: 3206 - 3216.

Full Paper
19483198 | recombinant UbcH7

Cooper, E.M., et al. (2009) "K63-specific deubuiquitination by two JAMM/MPN+ complexes:BRISC-associated Brcc-36 and proteasomal Poh1." EMBO J. 28(6) 621-631.

Full Paper
19214193 | UbcH12

Koodathingal, P., et al. (2009) "ATP-dependent Proteases Differ Substantially in Their Ability to Unfold Globular Proteins." J. Biol. Chem. 284: 18674-18684.

Full Paper
19383601 | recombinant UbcH5a

Maerki, S., et al. (2009) “The Cul3–KLHL21 E3 ubiquitin ligase targets Aurora B to midzone microtubules in anaphase and is required for cytokinesis.” J. Cell Biol. 187: 791 - 800.

Full Paper
19995937 | E2

Mao, X,. et al. (2009) "GCN5 is a required cofactor for a ubiquitin ligase that targets NF-kB/RelA." Genes & Dev. 23: 849-861.

Full Paper
19339690 | E2 (UbcH5a)

Mersman, D.P., et al. (2009) "Poly-ubiquitination of the demethylase Jhd2 controls histone methylation and gene expression." Genes & Dev. 23: 951-962.

Full Paper
19346402 | UbcH5a (human E2)

Nakao, R., et al. (2009) "Ubiquitin ligase Cbl-b is a negative regulator for IGF-1 signaling during muscle atrophy caused by unloading." Mol. Cell. Biol.: MCB.01347-08.

Full Paper
19546233 | UbcH7

Quezada, C.M., et al. (2009) "A family of Salmonella virulence factors functions as a distinct class of auto-regulated E3 ubiquitin ligases." PNAS 106 (12): 4864-4869.

Full Paper
19273841 | UbcH5b

Wang, X., et al. (2009) “Ube2j2 ubiquitinates hydroxylated amino acids on ER-associated degradation substrates.” J. Cell Biol. 187: 655 - 668.

Full Paper
19951915 | UbcH5a, His6-UbcH13–Uev1a complex

Wawrzynow, B., et al. (2009) "A Function for the RING Finger Domain in the Allosteric Control of MDM2 Conformation and Activity." J. Biol. Chem. 284: 11517-11530.

Full Paper
19188367 | UbcH5a (E2-616)

Bekker-Jensen, S., et al. (2009) "HERC2 coordinates ubiquitin-dependent assembly of DNA repair factors on damaged chromosomes.” Nature cell biology 12(1): 80–86.

20023648 | Ubc13–Mms2 complex

Jin, Z., et al. (2009) "Cullin3-Based Polyubiquitination and p62-Dependent Aggregation of Caspase-8 Mediate Extrinsic Apoptosis Signaling.” Cell 137(4): 721–735.

Full Paper
19427028 | recombinant human E2 (UbcH13/Uev1a), recombinant human E2 (UbcH5a), recombinant human E2 (UbcH7)

Kim, A.H., et al. (2009) "A centrosomal Cdc20-APC pathway controls dendrite morphogenesis in postmitotic neurons.” Cell 136(2): 322–336.

Full Paper
19167333 | UbcH10

Rybak, A., et al. (2009) "The let-7 target gene mouse lin-41 is a stem cell specific E3 ubiquitin ligase for the miRNA pathway protein Ago2.” Nature Cell Biology 11(12): 1411–1420.

19898466 | E2 Ubch10, Ubch2, Ubch5a, Ubch5b, Ubch5c,Ubch6, Ubch7

Siepe, D. and Jentsch, S. (2009) "Prolyl isomerase Pin1 acts as a switch to control the degree of substrate ubiquitylation.” Nature Cell Biology 11(8): 967–972.

19597489 | recombinant E2 (UbcH5c)

Singh, R.K., et al. (2009) "Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis.” Nature cell biology 11(8): 925–933.

Full Paper
19578373 | E2 enzyme recombinant GST–UbcH5A (human)

Soucy, T.A., et al. (2009) "An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer.” Nature 458(7239): 732–736.

19360080 | Ubc10

Stewart, G.S., et al. (2009) "The RIDDLE Syndrome Protein Mediates a Ubiquitin-Dependent Signaling Cascade at Sites of DNA Damage.” Cell 136(3): 420–434.

19203578 | indicated E2 enzymes

Balastik, M., et al. (2008) "Deficiency in ubiquitin ligase TRIM2 causes accumulation of neurofilament light chain and neurodegeneration." PNAS 105 (33): 12016-12021.

Full Paper
18687884 | different E2s

Chiang M-H., et al. (2008) "Ubiquitin-Conjugating Enzyme UBE2D2 Is Responsible for FBXW2 (F-Box and WD Repeat Domain Containing 2)-Mediated Human GCM1 (Glial Cell Missing Homolog 1) Ubiquitination and Degradation." Biol Reprod 79(5):914-920.

Full Paper
18703417 | HeLa S-100 fraction

Daulny, A., et al. (2008) "Modulation of RNA polymerase II subunit composition by ubiquitylation." PNAS 105(50): 19649-19654.

Full Paper
19064926 | human E2 (UbcH5a)

Gallegos J.R., et al. (2008) “SCF TrCP1 Activates and Ubiquitylates TAp63γ.” J. Biol. Chem. 283: 66-75.

Full Paper
17965458 | UbcH3

Karagianni, P., et al. (2008) "ICBP90, a Novel Methyl K9 H3 Binding Protein Linking Protein Ubiquitination with Heterochromatin Formation." Mol. Cell. Biol. 28: 705-17.

Full Paper
17967883 | E2

Kawahara, K., et al. (2008) "Alpha-Synuclein Aggregates Interfere with Parkin Solubility and Distribution: ROLE IN THE PATHOGENESIS OF PARKINSON DISEASE." J. Biol. Chem. 283: 6979-6987.

Full Paper
18195004 | UbcH7

Kim, A.Y., et al. (2008) “SCCRO (DCUN1D1) Is an Essential Component of the E3 Complex for Neddylation.” J. Biol. Chem. 283(48): 33211-33220.

Full Paper
18826954 | Ubc12

Lovly C.M., et al. (2008) "Regulation of Chk2 Ubiquitination and Signaling through Autophosphorylation of Serine 379." Mol. Cell. Biol. 28(19): 5874-5885.

Full Paper
18644861 | human recombinant E2 (UbcH5c) enzyme

Brooks, W.S., et al. (2008) "G2E3 Is a Dual Function Ubiquitin Ligase Required for Early Embryonic Development." J. Biol. Chem. 283(32): 22304-22315.

Full Paper
18511420 | UbcH1, UbcH2, UbcH3, UbcH5a, UbcH7, UbcH9, or UbcH10

Conze, D.B., et al. (2008) “Lys63-Linked Poly-ubiquitination of IRAK-1 Is Required for Interleukin-1 Receptor- and Toll-Like Receptor-Mediated NF-κB Activation.” Mol. Cell. Biol. 28: 3538-47.

Full Paper
18347055 | Ubc13, Uev1A

Heuzé, M.L., et al. (2008) "ASB2 targets filamins A and B to proteasomal degradation." Blood 112(13): 5130-5140.

Full Paper
18799729 | Ubc12, UbcH5a

Koh, M.Y., et al. (2008) "Hypoxia-Associated Factor, a Novel E3-Ubiquitin Ligase, Binds and Ubiquitinates Hypoxia-Inducible Factor 1, Leading to Its Oxygen-Independent Degradation." Mol. Cell. Biol. 28 (23):7081-7095.

Full Paper
18838541 | human recombinant E2

Koutelou, E., et al. (2008) "Neuralized-like 1 (Neurl1) Targeted to the Plasma Membrane by N-Myristoylation Regulates the Notch Ligand Jagged1." J. Biol. Chem. 283: 3846-53.

Full Paper
18077452 | GST-tagged human UbcH2

Lagarou, A., et al. (2008) "KDM2 couples histone H2A ubiquitylation to histone H3 demethylation during Polycomb group silencing." Genes & Dev. 22(20): 2799-2810.

Full Paper
18923078 | ubiquitin-conjugating enzyme UbcH5c

Lui, Q.Y., et al. (2008). "A novel brain-enriched E3 ubiquitin ligase RNF182 is up-regulated in the brains of Alzheimer’s patients and targets ATP6VOC for degradation." Molec. Degen. 3:4.

Full Paper
18298843 | Ubc5a

Maguire M., et al. (2008) "MDM2 Regulates Dihydrofolate Reductase Activity through Monoubiquitination." Canc. Res. 68: 3232-3242.

Full Paper
18451149 | UbcH5B

Malakhova O.A. and Zhang D-E. (2008) "ISG15 Inhibits Nedd4 Ubiquitin E3 Activity and Enhances the Innate Antiviral Response." J. Biol. Chem. 283: 8783-8787.

Full Paper
18287095 | Ub-conjugating enzyme UbcH6

Menon S., et al. (2008). "Association of SAP130/SF3b-3 with Cullin-RING ubiquitin ligase complexes and its regulation by the COP9 signalosome.” BMC Biochem. 9:1.

Full Paper
18173839 | GST-UbcH5b

Ohtsubo M., et al. (2008) "Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity." PNAS 105(30): 10396-10401.

Full Paper
18650381 | ubiquitin-conjugating enzyme UbcH5c

Pebernard, S., et al. (2008) "Nse1 RING-like Domain Supports Functions of the Smc5-Smc6 Holo-complex in Genome Stability." Mol. Biol. Cell 19(10): 4099-4109.

Full Paper
18667531 | 6xHis-UbcH10, 6xHis-UbcH3, UbcH13/Uev1a, UbcH2, UbcH5a

Pierre S., et al. (2008) "Toponomics analysis of functional interactions of the ubiquitin ligase PAM during spinal nociceptive processing." Mol. Cell. Proteomics. 7(12)2475-2485.

Full Paper
18753128 | E2 proteins

Sehat B, et al. (2008). "Identification of c-Cbl as a New Ligase for Insulin-like Growth Factor-I Receptor with Distinct Roles from Mdm2 in Receptor Ubiquitination and Endocytosis." Canc. Res. 68: 5669-5677.

Full Paper
18632619 | E2 (UbcH5B), mutated ubiquitin

Sekine K., et al. (2008). "Small Molecules Destabilize cIAP1 by Activating Auto-ubiquitylation." J. Biol. Chem. 283(14): 8961-8968.

Full Paper
18230607 | human UbcH5a

Tatematsu, K., et al. (2008) "Identification of Ubiquitin Ligase Activity of RBCK1 and Its Inhibition by Splice Variant RBCK2 and Protein Kinase C[beta]." J. Biol. Chem. 283: 11575-85.

Full Paper
18303026 | UbcH7

Umebayashi K., et al. (2008) "Ubc4/5 and c-Cbl Continue to Ubiquitinate EGF Receptor after Internalization to Facilitate Polyubiquitination and Degradation." Mol. Biol. Cell. 19(8): 3454-3462.

Full Paper
18508924 | GST-UbcH5A, UbcH5C, UbcH7

Varfolomeev E., et al. (2008) "c-IAP1 and c-IAP2 Are Critical Mediators of Tumor Necrosis Factor [alpha](TNF[alpha])-induced NF-κB Activation." J. Biol. Chem. 283(36): 24295-24299.

Full Paper
18621737 | UbcH13 (always used in complex with Uev1), UbcH5a

Lu, K., et al. (2008) "Targeting WW domains linker of HECT-type ubiquitin ligase Smurf1 for activation by CKIP-1." Nature cell biology 10(8): 994–1002.

18641638 | UbcH5c

Winter, M., et al. (2008) "Control of HIPK2 stability by ubiquitin ligase Siah-1 and checkpoint kinases ATM and ATR." Nature cell biology 10(7): 812–824.

18536714 | UbcH5b

Zhang, Z. and Zhang, R. (2008) "Proteasome activator PA28[gamma] regulates p53 by enhancing its MDM2-mediated degradation.” The EMBO journal 27(6): 852–864.

Full Paper
18309296 | UbcH5b

Catic A., et al. (2007) "Screen for ISG15-cross-reactive Deubiquitinases." PLoS ONE 2(7): E769.

Full Paper
17653289 | UbcH8

Hattori, T., et al. (2007) "Pirh2 promotes ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor p27kip1." Can. Res. 67: 10789-10795.

Full Paper
18006823 | UbcH5b

Braun, M. A., et al. (2007). "Identification of Rkr1, a Nuclear RING Domain Protein with Functional Connections to Chromatin Modification in Saccharomyces cerevisiae." Mol. Cell. Biol. 27(8): 2800-2811.

Full Paper
17283062 | ubiquitin-conjugating enzyme (human recombinant UbcH5a)

Dul, B. E. and N. C. Walworth (2007). "The Plant Homeodomain Fingers of Fission Yeast Msc1 Exhibit E3 Ubiquitin Ligase Activity." J. Biol. Chem. 282(25): 18397-18406.

Full Paper
17456468 | human E2

Hong, K. U., et al. (2007). "Functional Importance of the Anaphase-Promoting Complex-Cdh1-Mediated Degradation of TMAP/CKAP2 in Regulation of Spindle Function and Cytokinesis." Mol. Cell. Biol. 27(10): 3667-3681.

Full Paper
17339342 | His6-UbcH10 (E2)

Jang, I.-C., et al. (2007). "Independent and interdependent functions of LAF1 and HFR1 in phytochrome A signaling." Genes & Dev. 21(16): 2100-2111.

| human E2 UbcH5b

Kamynina, E., et al. (2007). "Regulation of Proto-Oncogenic Dbl by Chaperone-Controlled, Ubiquitin-Mediated Degradation.” Mol. Cell. Biol. 27(5): 1809-1822.

Full Paper
17178836 | E2

Butler, M. P., et al. (2007). "Kinase active IRAKs promote polyubiquitination and degradation of the Pellino family: Direct evidence for Pellino proteins being E3 ubiquitin ligases." J. Biol. Chem. 282:29729-29737.

Full Paper
17675297 | E2 (UbcH13/Uev1a)

Kong, H. J., et al. (2007). "Cutting Edge: Autoantigen Ro52 Is an Interferon Inducible E3 Ligase That Ubiquitinates IRF-8 and Enhances Cytokine Expression in Macrophages." J. Immunol. 179(1): 26-30.

Full Paper
17579016 | E2

Lu, Z., et al. (2007). "Regulation of synaptic growth and maturation by a synapse-associated E3 ubiquitin ligase at the neuromuscular junction." J. Cell Biol. 177(6): 1077-1081.

Full Paper
17576800 | E2

Olzmann, J.A., et al. (2007) "Parkin-mediated K63-linked polyubiquitination targets misfolded DJ-1 to aggresomes via binding to HDAC6." J. Cell Biol. 178(6): 1025-1038.

Full Paper
17846173 | E2 ubiquitin-conjugating enzyme (UbcH13/Uev1a)

Pick, E., et al. (2007). "Mammalian DET1 Regulates Cul4A Activity and Forms Stable Complexes with E2 Ubiquitin-Conjugating Enzymes.” Mol. Cell. Biol. 27(13): 4708-4719.

Full Paper
17452440 | anti-UbcH12, anti-UbcH5

Pick, E., et al. (2007). "Mammalian DET1 Regulates Cul4A Activity and Forms Stable Complexes with E2 Ubiquitin-Conjugating Enzymes.” Mol. Cell. Biol. 27(13): 4708-4719.

Full Paper
17452440 | UbcH5c

Sargin, B., C. Choudhary, et al. (2007). "Flt3-dependent transformation by inactivating c-Cbl mutations in AML." Blood 110(3): 1004-1012.

Full Paper
17446348 | E2

Skaar, J. R., et al. (2007). "PARC and CUL7 Form Atypical Cullin RING Ligase Complexes.” Cancer Res. 67(5): 2006-2014.

Full Paper
17332328 | E2

Sun, Z., et al. (2007). "Keap1 Controls Post-Induction Repression of the Nrf2-Mediated Antioxidant Response by Escorting Nuclear Export of Nrf2." Mol. Cell. Biol.: 27(18):6334-6349.

Full Paper
17636022 | E2-UbcH5a

Zhao, Y., et al. (2007). "Tumor Necrosis Factor Receptor 2 Signaling Induces Selective c-IAP1-dependent ASK1 Ubiquitination and Terminates Mitogen-activated Protein Kinase Signaling." J. Biol. Chem. 282(11): 7777-7782.

Full Paper
17220297 | UbcH5b

Fortier, J. M. and J. Kornbluth (2006). "NK Lytic-Associated Molecule, Involved in NK Cytotoxic Function, Is an E3 Ligase." J. Immunol. 176(11): 6454-6463.

Full Paper
16709802 | Polyclonal UbcH8 Ab

Germano, S., D. et al. (2006). "Geldanamycins Trigger a Novel Ron Degradative Pathway, Hampering Oncogenic Signaling." J. Biol. Chem. 281: 21710-21719.

Full Paper
16740632 | E2 (UbcH5a)

Gurtan, A. M., et al. (2006) "The WD40 Repeats of FANCL Are Required for Fanconi Anemia Core Complex Assembly." J. Biol. Chem. 281(16): 10896-10905.

Full Paper
16474167 | UBCH5a

He, F., B. J. Fenner, et al. (2006). "White Spot Syndrome Virus Open Reading Frame 222 Encodes a Viral E3 Ligase and Mediates Degradation of a Host Tumor Suppressor via Ubiquitination." J. Virol. 80(8): 3884-3892.

Full Paper
16571805 | E2

Ji, P., et al. (2006). "Skp2 Contains a Novel Cyclin A Binding Domain That Directly Protects Cyclin A from Inhibition by p27kip1." J. Biol. Chem. 281(33): 24058-24069.

Full Paper
16774918 | E2(Cdc34)

Hiramatsu, Y., K. Kitagawa, et al. (2006). "Degradation of Tob1 Mediated by SCFSkp2-Dependent Ubiquitination." Cancer Res. 66(17): 8477-8483.

Full Paper
16951159 | E2 (UbcH3), E2 (UbcH5b)

Yurchenko, V., et al. (2006). "SUMO Modification of Human XRCC4 Regulates Its Localization and Function in DNA Double-Strand Break Repair." Mol. Cell. Biol. 26(5): 1786-1794.

Full Paper
16478998 | Ubc9

Kim, S. T., et al. (2006). "Exogenous Free Ubiquitin Enhances Lily Pollen Tube Adhesion to an in Vitro Stylar Matrix and May Facilitate Endocytosis of SCA (Stigma/stylar Cysteine-rich Adhesin)." Plant Physiology: 142: 984-992.

Full Paper
16998086 | N-terminal LUb

Lo, S.-C. and M. Hannink (2006). "CAND1-Mediated Substrate Adaptor Recycling Is Required for Efficient Repression of Nrf2 by Keap1." Mol. Cell. Biol. 26(4): 1235-1244.

Full Paper
16449638 | E2-UbcH5a

Lo, S.-C. and M. Hannink (2006). "PGAM5, a Bcl-XL-interacting protein, is a novel substrate for the redox-regulated Keap1-dependent ubiquitin ligase complex." J. Biol. Chem.: 281:37893-37903.

Full Paper
17046835 | E2-UbcH5a

Nelson, R. F., et al. (2006). "A Novel Route for F-box Protein-mediated Ubiquitination Links CHIP to Glycoprotein Quality Control." J. Biol. Chem. 281(29): 20242-20251.

Full Paper
16682404 | GST-UbcH5a, GST-UbcH5b, GST-UbcH5c, His-UbcH6, UbcH7

Nelson, R. F., et al. (2006). "A Novel Route for F-box Protein-mediated Ubiquitination Links CHIP to Glycoprotein Quality Control." J. Biol. Chem. 281(29): 20242-20251.

Full Paper
16682404 | GST-UbcH5a, GST-UbcH5b, GST-UbcH5c, His-UbcH6, UbcH7

Yurchenko, V., et al. (2006). "SUMO Modification of Human XRCC4 Regulates Its Localization and Function in DNA Double-Strand Break Repair." Mol. Cell. Biol. 26(5): 1786-1794.

Full Paper
16478998 | SAE1, SAE2, Ubc9 enzyme

Laine, A., et al. (2006) "Regulation of p53 Localization and Activity by Ubc13." Mol. Cell. Biol. 26(23): 8901-8913.

Full Paper
17000756 | His-Ubc13, Uev1A

Chung A. S., et al. (2005) "Ankyrin Repeat and SOCS Box 3 (ASB3) Mediates Ubiquitination and Degradation of Tumor Necrosis Factor Receptor II." Mol. Cell. Biol. 25(11): 4716-4726.

Full Paper
15899873 | ubiquitin-conjugating enzyme (E2-UbcH5a)

Gazdoiu S., et al. (2005) "Proximity-induced activation of human Cdc34 through heterologous dimerization." PNAS 102(42): 15053-15058.

Full Paper
16210246 | APP-BP1, Uba3

Gentry M. S., et al. (2005) "From The Cover: Insights into Lafora disease: Malin is an E3 ubiquitin ligase that ubiquitinates and promotes the degradation of laforin." PNAS 102(24): 8501-8506.

Full Paper
15930137 | human E2

Horn P. J., et al. (2005) "A Rik1-associated, cullin-dependent E3 ubiquitin ligase is essential for heterochromatin formation." Genes & Dev. 19(14): 1705-1714.

Full Paper
6024659 | recombinant human UbcH5b

Jang I.-C., et al. (2005) "HFR1 is targeted by COP1 E3 ligase for post-translational proteolysis during phytochrome A signaling." Genes & Dev. 19(5): 593-602.

Full Paper
15741320 | human E2 UbcH5b

Kim D. W., et al. (2005) "The Shigella flexneri effector OspG interferes with innate immune responses by targeting ubiquitin-conjugating enzymes." PNAS 102(39): 14046-14051.

Full Paper
http://www.pnas.org/content/102/39/14046.long | anti-UbcH5

Shirogane T., et al. (2005) "SCF[beta]-TRCP Controls Clock-dependent Transcription via Casein Kinase 1-dependent Degradation of the Mammalian Period-1 (Per1) Protein." J. Biol. Chem. 280(29): 26863-26872.

Full Paper
15917222 | Ubc5

Yan C., et al. (2005) "Activating transcription factor 3, a stress sensor, activates p53 by blocking its ubiquitination." EMBO J. 24(13): 2425-2435.

Full Paper
15933712 | E2

Chen L. and Madura K. (2005) "Increased Proteasome Activity, Ubiquitin-Conjugating Enzymes, and eEF1A Translation Factor Detected in Breast Cancer Tissue." Cancer Res. 65(13): 5599-5606.

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15994932 | anti-Ubch1 (E2)

Fotia, A.B., et al. (2005). "The ubiquitin-protein ligases Nedd4 and Nedd4-2 show similar ubiquitin-conjugating enzyme specificities." Intl. J. Biochem. Cell. Biol. 38(3): 472-479.

16337426 | GST-UbcH5b, GST-UbcH5c, UbcH10, UbcH3, UbcH5b

Lois L. M. and Lima C. D. (2005) "Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1." EMBO J. 24(3): 439-451.

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15660128 | Ubc12, Ubc5, Ubc7

Wang Z., et al. (2005) "RING-H2 Protein WSSV249 from White Spot Syndrome Virus Sequesters a Shrimp Ubiquitin-Conjugating Enzyme, PvUbc, for Viral Pathogenesis." J. Virol. 79(14): 8764-8772.

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15994770 | human GST-UbcH5b / human UbcH1, human UbcH10, human UbcH2, human UbcH3, human UbcH5a, human UbcH9

Yogosawa S., et al. (2005) "Ubiquitylation and Degradation of Serum-inducible Kinase by hVPS18, a RING-H2 Type Ubiquitin Ligase." J. Biol. Chem. 280(50): 41619-41627.

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16203730 | E2-enzyme mixture

Zhang D. D., et al. (2005) "Ubiquitination of Keap1, a BTB-Kelch Substrate Adaptor Protein for Cul3, Targets Keap1 for Degradation by a Proteasome-independent Pathway." J. Biol. Chem. 280(34): 30091-30099.

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15983046 | E2-UbcH5a

Zhang Y., et al. (2005) "Recognition and Ubiquitination of Salmonella Type III Effector SopA by a Ubiquitin E3 Ligase, HsRMA1." J. Biol. Chem. 280(46): 38682-38688.

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16176924 | UbcH5a

Adachi K., et al. (2004) "Ubiquitylation of Nascent Globin Chains in a Cell-free System." J. Biol. Chem. 279(40): 41767-41774.

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15297454 | E2

Bartee E., et al. (2004) "Downregulation of Major Histocompatibility Complex Class I by Human Ubiquitin Ligases Related to Viral Immune Evasion Proteins." J. Virol. 78(3): 1109-1120.

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14722266 | UbcH2

Fotia A. B., et al. (2004) "Regulation of Neuronal Voltage-gated Sodium Channels by the Ubiquitin-Protein Ligases Nedd4 and Nedd4-2." J. Biol. Chem. 279(28): 28930-28935.

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15123669 | UbcH5b

Wei, W., et al.(2004) "Degradation of the SCF component Skp2 in cell-cycle phase G1 by the anaphase promoting complex." Nature 428: 194-198.

15014503 | UbCH-10

Yang Q.-H. and Du C. (2004) "Smac/DIABLO Selectively Reduces the Levels of c-IAP1 and c-IAP2 but Not That of XIAP and Livin in HeLa Cells." J. Biol. Chem. 279(17): 16963-16970.

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14960576 | various recombinant human E2

Burstein E., et al. (2004) "A novel role for XIAP in copper homeostasis through regulation of MURR1." EMBO J. 23(1): 244-254.

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14685266 | E2

Mudgil Y., et al. (2004) "A Large Complement of the Predicted Arabidopsis ARM Repeat Proteins Are Members of the U-Box E3 Ubiquitin Ligase Family." Plant Physiology 134(1): 59-66.

Full Paper
14657406 | human UBC10, human UBC2A, human UBC3, human UBC5A, human UBC5B, human UBC6, human UBC7

Oh K.-J., et al. (2004) "The Papillomavirus E7 Oncoprotein Is Ubiquitinated by UbcH7 and Cullin 1- and Skp2-Containing E3 Ligase." J. Virol. 78(10): 5338-5346.

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15113913 | ubiquitin-conjugating enzyme E2

Peng H.-M., et al. (2004) "Ubiquitylation of Neuronal Nitric-oxide Synthase by CHIP, a Chaperone-dependent E3 Ligase." J. Biol. Chem. 279(51): 52970-52977.

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15466472 | GST-tagged UbcH5a

Plafker S. M., et al. (2004) "Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import." J. Cell Biol. 167(4): 649-659.

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15545318 | anti-UbcH6

Secombe J. and Parkhurst S.M. (2004) "Drosophila Topors Is a RING Finger-containing Protein That Functions as a Ubiquitin-protein Isopeptide Ligase for the Hairy Basic Helix-Loop-Helix Repressor Protein." J. Biol. Chem. 279(17): 17126-17133.

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14871887 | human UbcH5a

Wang W., et al. (2004) "Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail." J. Biol. Chem. 279(49): 51362-51369.

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Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. | APP-BP1, Uba3

Yanagawa Y., et al. (2004) "Arabidopsis COP10 forms a complex with DDB1 and DET1 in vivo and enhances the activity of ubiquitin conjugating enzymes." Genes & Dev. 18(17): 2172-2181.

Full Paper
15342494 | UbcH13, Uev1

Yang Q.-H. and Du C. (2004) "Smac/DIABLO Selectively Reduces the Levels of c-IAP1 and c-IAP2 but Not That of XIAP and Livin in HeLa Cells." J. Biol. Chem. 279(17): 16963-16970.

Full Paper
14960576 | various recombinant human E2, UbcH5a

Yang, Q.-H. and Du C.(2004) "Smac/DIABLO Selectively Reduces the Levels of c-IAP1 and c-IAP2 but Not That of XIAP and Livin in HeLa Cells." J. Biol. Chem. 279(17): 16963-16970.

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14960576 | various recombinant human E2

Yao D., et al. (2004) "Nitrosative stress linked to sporadic Parkinson's disease: S-nitrosylation of parkin regulates its E3 ubiquitin ligase activity." PNAS 101(29): 10810-10814.

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15252205 | E2 ubiquitin-conjugating enzyme (UbcH7)

Zhang D. D., et al. (2004) "Keap1 Is a Redox-Regulated Substrate Adaptor Protein for a Cul3-Dependent Ubiquitin Ligase Complex." Mol. Cell. Biol. 24(24): 10941-10953.

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15572695 | E2-UbcH5a

Goto, E., et al. (2003). "c-MIR, a human E3 ubiquitin ligase, is a functional homolog of herpesvirus proteins MIR1 and MIR2 and has similar activity." J. Biol. Chem. 278(17): 14657-14668.

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12582153 | UbcH5a

Hu S. and Yang X. (2003). "Cellular inhibitor of apoptosis1 and 2 are ubiquitin ligase for the apoptosis inducer Smac/DIABLO." J. Biol. Chem. 278(12): 10055-10060.

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12525502 | ubiquitin-conjugating enzyme (E2) GST-UbcH5c

Jones, J.M., et al. (2003) "Autoubiquitylation of the V(D)J recombinase protein RAG1." PNAS 100(26): 15446-15451.

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14671314 | human E2 enzymes

Osaka, H., et al. (2003). "Ubiquitin carboxyl-terminal hydrolases L1 binds to and stabilizes monoubiquitin in neuron." Hum. Molec. Genet. 12: 1945-1958.

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12913066 | (Ub)2, (Ub)3, (Ub)4

Pan Y. and Chen J. et al. (2003). "MDM2 promotes ubiquitination and degradation of MDMX." Mol. Cell. Biol. 23(15): 5113-5121.

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12860999 | GST-Ubc5Hb

Sakamoto, K.M., et al. (2003). "Development of Protacs to target cancer-promoting proteins for ubiquitination and degradation." Mol. Cell. Proteomics 2: 1350-1358.

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14525958 | Ubch5a

Takeyama, K., et al. (2003). "The BAL-binding protein BBAP and related Deltex family members exhibit ubiquitin-protein isopeptide ligase activity." J. Biol. Chem. 278(24): 21930-21937.

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12670957 | HbcH5a, UbcH5b, UbcH5c

Yurchenko, V., et al. (2003). "The RAG1 N-terminal domain is an E3 ubiquitin ligase." Genes and Dev. 17(5): 581-585.

Full Paper
12629039 | UbcH10, UbcH2, UbcH3, UbcH5a, UbcH5b, UbcH5c, UbcH6, UbcH7