Product References - Deconjugating

Boston Biochem's products have been used successfully in hundreds of UPP-related publications. We have listed our most recent references below to provide our customers with an exceptional resource for product use information. The references are organized to mirror our product divisions for easy location. Products referenced in each article are listed below in red. Please inquire to request archives as we have curated references back to 2003.

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23536702 | His6-USP2CD (UBP41 catalytic domain)
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23375434 | human recombinant isopeptidase T

Cotto-Rios, X.M. et al. (2012) "Deubiquitinases as a signaling target of oxidative stress." Cell Rep 2(6): 1475–1484.

23219552 | UbVME, recombinant ubiquitin chain K48-linked, UCH-L1, SENP1-CD, STAM, recombinant ubiquitin chain K63-linked, SUMO1-vs, USP7, AMSH,

Zang, Y. et al. (2012) "Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage." Rice 5(1): 24.

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10.1186/1939-8433-5-24 | E1 enzyme, K48R ubiquitin, K63R ubiquitin, reaction buffer, Ub, Ubc13, Uev1A, ubiquitin-AMC, nonbiotinylated USP2 catalytic domain, USP10, USP47, USP5, USP7

Reverdy, C. et al. (2012) "Discovery of Specific Inhibitors of Human USP7/HAUSP Deubiquitinating Enzyme." Chem. Biol. 19(4): 467–477.

22520753 | UCH-L1, UCH-L3, USP2, USP20, USP5, E-340, E-325, E-506, E-344, E-322

Issaenko, O.A. and Amerik, A.Y. (2012) "Chalcone-based small-molecule inhibitors attenuate malignant phenotype via targeting deubiquitinating enzymes." Cell cycle (Georgetown, Tex.) 11(9).

22510564 | purified human DUB, purified USP5

Shi, T. et al. (2012) "Identification Of Small Molecule TRABID Deubiquitinase Inhibitors By Computation-Based Virtual Screen." BMC chemical biology 12(1): 4.

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22584113 | A20 catalytic domain

Capodagli, G., et al. (2011) “Structural analysis of a viral ovarian tumor domain protease from the Crimean-Congo Hemorrhagic Fever virus in complex with covalently bonded ubiquitin.” Journal of Virology, 85: 3621-3630.

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21228232 | Ub-AMC

Chen, B.B., et al (2011) “Calmodulin antagonizes a calcium-activated SCF ubiquitin E3 ligase subunit, FBXL2, to regulate surfactant homeostasis.” Molecular and Cellular Biology: doi:10.1128/MCB.00723-10.” 

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21343341 | ubiquitin aldehyde

James, T., et al. (2011) “Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease.” PNAS, 108: 2222-2227.

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21245344 | ISG15–AMC

Cao, X., et al. (2011) “WW Domain-containing E3 Ubiquitin Protein Ligase 1 (WWP1) Delays Cellular Senescence by Promoting p27 Kip1 Degradation in Human Diploid Fibroblasts.” J. Biol. Chem. 286: 33447 - 33456.

21795702 | Wild-type ubiquitin

Cosper, P. and Leinwand, L. (2011) “Cancer Causes Cardiac Atrophy and Autophagy in a Sexually Dimorphic Manner.” Cancer Research, 71: 1710.

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21163868 | ubiquitin aldehyde

Cotto-Rios, X.M., et al. (2011) “APC/CCdh1-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage.” J. Cell Biol. 194: 177 - 186.

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21768287 | HA-UbVS probe

Huang, Z., et al. (2011) “Deubiquitylase HAUSP stabilizes REST and promotes maintenance of neural progenitor cells.” Nature Cell Biology, 13: 142-152.

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21258371 | ubiquitin aldehyde

Lu, J., et al. (2011) “RNF170 Protein, an Endoplasmic Reticulum Membrane Ubiquitin Ligase, Mediates Inositol 1,4,5-Trisphosphate Receptor Ubiquitination and Degradation.” J. Biol. Chem. 286: 24426 - 24433.

21610068 | HA-ubiquitin

Lu, K., et al. (2011) “Pivotal role of the SMURF1 C2 domain in substrate selection.” The Journal of Biological Chemistry: doi:10.1074/jbc.M110.211979.

21402695 | HA–ubiquitin

Stringer, D. and Piper, R. (2011) “A single ubiquitin is sufficient for cargo protein entry into MVBs in the absence of ESCRT Ubiquitination.” JCB, 192: 229-242.

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21242292 | Ub-Aldehyde

Uchida, S., et al. (2011) “SCF[beta]TrCP mediates stress-activated MAPK-induced Cdc25B degradation.” J. Cell Sci. 124: 2816 - 2825.

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21807946 | ubiquitin-aldehyde

Wickliffe, K., et al. (2011) “The Mechanism of Linkage-Specific Ubiquitin Chain Elongation by a Single-Subunit E2.” Cell, 144: 769-781.

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21376237 | wild-type ubiquitin

Yang, K., et al. (2011) “Regulation of the Fanconi anemia pathway by a SUMO-like delivery network.” Genes & Dev. 25: 1847 - 1858.

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21896657 | Ub-AMC

Mansfeld, J., et al. (2011) "APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.” Nature cell biology 13(10): 1234–1243.

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21926987 | anti-UbcH10

Chenon, M., et al. (2011) "A viral deubiquitylating enzyme targets viral RNA-dependent RNA polymerase and affects viral infectivity.” The EMBO Journal 31(3): 741–753.

22117220 | Ub-AMC

Jones, M.J.K., et al. (2011) "Dysregulation of DNA polymerase [kappa] recruitment to replication forks results in genomic instability.” The EMBO Journal. 31(4):908-18.

| recombinant USP2 catalytic domain (DUB-CD)

Artavanis-Tsakonas, K., et al. (2010) “Characterization and Structural Studies of the Plasmodium falciparum Ubiquitin and Nedd8 Hydrolase UCHL3.” J. Biol. Chem. 285: 6857 - 6866.

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20042598 | Nedd8-AMC

Clapp, K. M., et al. (2010) “C331A Mutant of Neuronal Nitric-oxide Synthase Is Labilized for Hsp70/CHIP (C Terminus of HSC70-interacting Protein)-dependent Ubiquitination.” J. Biol. Chem. 285: 33642-33651.

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20729196 | ubiquitin aldehyde

de Thonel, A., et al. (2010) “HSP27 controls GATA-1 protein level during erythroid cell differentiation.” Blood116: 85 - 96.

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20410505 | ubiquitin-aldehyde

Geng, L., et al. (2010) “RAD18-mediated ubiquitination of PCNA activates the Fanconi anemia DNA repair network.” J. Cell Biol. 191: 249-257.

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20937699 | human recombinant UBE2T

Guo, Y., et al. (2010) “Blockade of the Ubiquitin Protease UBP43 Destabilizes Transcription Factor PML/RAR and Inhibits the Growth of Acute Promyelocytic Leukemia.” Cancer Res. 70: 9875-9885.

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20935222 | HA-ISG15-vinylmethyl ester (HA-ISG15-VME)

Hallows, K.R., et al. (2010) “Phosphopeptide Screen Uncovers Novel Phosphorylation Sites of Nedd4-2 That Potentiate Its Inhibition of the Epithelial Na+ Channel.” J. Biol. Chem. 285: 21671 - 21678.

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20466724 | HA-tagged ubiquitin

Kops, G., et al. (2010) "APC16 is a conserved subunit of the anaphase-promoting complex/cyclosome." J. Cell Sci. 123: 1623 - 1633.

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20392738 | HA-ubiquitin

Sánchez Manchinelly, S.A. (2010) “Mitotic Down-regulation of p190RhoGAP Is Required for the Successful Completion of Cytokinesis.” J. Biol. Chem. 285: 26923 - 26932.

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20534586 | ubiquitin aldehyde

An, J.Y., et al. (2010) “UBR2 mediates transcriptional silencing during spermatogenesis via histone ubiquitination.” PNAS 107: 1912 - 1917.

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20080676 | Ub tagged with ha

Li, X.S., et al. (2010) "Mammalian SWI/SNF-A Subunit BAF250/ARID1 Is an E3 Ubiquitin Ligase That Targets Histone H2B." Mol. Cell. Biol. 30: 1673 - 1688.

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20086098 | hemagglutinin antigen-tagged ubiquitin (HA-Ub)

Liang, J., et al. (2010) “MCP-induced protein 1 deubiquitinates TRAF proteins and negatively regulates JNK and NF-B signaling.” J. Exp. Med. 207: 2959-2973.

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21115689 | IsoT, ubiquitin aldehyde, ubiquitin-AFC

Mullen, J.R., et al. (2010) “Wss1 Is a SUMO-Dependent Isopeptidase That Interacts Genetically with the Slx5-Slx8 SUMO-Targeted Ubiquitin Ligase.” Mol. Cell. Biol. 30: 3737 - 3748.

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20516210 | Usp2

Nie, J., et al. (2010) “Smad Ubiquitylation Regulatory Factor 1/2 (Smurf1/2) Promotes p53 Degradation by Stabilizing the E3 Ligase MDM2.” J. Biol. Chem. 285: 22818 - 22830.

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20484049 | HA-Ub

Seyfried, N.T., et al. (2010) "Multiplex SILAC Analysis of a Cellular TDP-43 Proteinopathy Model Reveals Protein Inclusions Associated with SUMOylation and Diverse Polyubiquitin Chains." Mol. Cell. Proteomics. 9: 705 - 718.

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20047951 | sentrin-specific endopeptidase 2 (SENP2)

Song, E.J., et al. (2010) “The Prp19 complex and the Usp4Sart3 deubiquitinating enzyme control reversible ubiquitination at the spliceosome.” Genes & Dev. 24: 1434 - 1447.

| ubiquitin-AMC

Alvarez, S.E., et al. (2010) "Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2.” Nature 465(7301): 1084–1088.

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20577214 | wild-type ubiquitin

Fujita, K., et al. (2010) "Positive feedback between p53 and TRF2 during telomere-damage signalling and cellular senescence.” Nature cell biology 12(12): 1205–1212.

21057505 | ubiquitin aldehyde

Lee, B.H., et al. (2010) "Enhancement of proteasome activity by a small-molecule inhibitor of USP14.” Nature 467(7312): 179–184.

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20829789 | UCH-L1, UCH-L3, USP15, USP2 catalytic domain (CD), USP7(CD)

Bernal-Bayard, J. and Ramos-Morales, F. (2009) “Salmonella Type III Secretion Effector SlrP Is an E3 Ubiquitin Ligase for Mammalian Thioredoxin.” J. Biol. Chem. 284: 27587 - 27595.

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19690162 | HA-tagged ubiquitin

Burnett, B.G., et al. (2009) "Regulation of SMN Protein Stability." Mol. Cell. Biol. 29(5): 1107-1115.

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19103745 | ubiquitin aldehyde

Christopher, B., et al. (2009) “The EBV Deubiquitinating Enzyme, BPLF1, Reduces EBV Ribonucleotide Reductase Activity.” J. Virol. JVI.02195-08v1 .

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19244336 | Ub C-terminal hydrolase (UCH-L1)

Colland, F., et al. (2009) “Small-molecule inhibitor of USP7/HAUSP ubiquitin protease stabilizes and activates p53 in cells.” Mol. Cancer Ther. 8: 2286 - 2295.

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19671755 | Ubiquitin COOH-terminal hydrolase-L1 (UCH-L1)

Kim, E.T., et al. (2009) “Cleavage Specificity of the UL48 Deubiquitinating Protease Activity of Human Cytomegalovirus and the Growth of an Active-Site Mutant Virus in Cultured Cells.” J. Virol. 83: 12046 - 12056.

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19759126 | K48-poly-Ub

Longerich, S., et al. (2009) “FANCI Binds Branched DNA and Is Monoubiquitinated by UBE2T-FANCL.” J. Biol. Chem. 284: 23182 - 23186.

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19589784 | HA epitope-tagged human ubiquitin

Misaghi, S., et al. (2009) "Association of C-Terminal Ubiquitin Hydrolase BRCA1-Associated Protein 1 with Cell Cycle Regulator Host Cell Factor 1." Mol. Cell. Biol. 29: 2181-2192.

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19188440 | UCH-L3

Nishikawa, H., et al. (2009) "BRCA1-Associated Protein 1 Interferes with BRCA1/BARD1 RING Heterodimer Activity." Canc. Res. 69 (1):111-119.

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19117993 | isopeptidase T

Popp, M.W., et al. (2009) "Substrate Filtering by the Active Site Crossover Loop in UCHL3 Revealed by Sortagging and Gain-of-function Mutations." J. Biol. Chem. 284 (6): 3593-3602.

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19047059 | Ub C-terminal 7-amido-4-methylcoumarin (Ub-AMC), USP8

Shanks. J., et al. (2009) "Burkholderia mallei tssM Encodes a Putative Deubiquitinase That Is Secreted and Expressed inside Infected RAW 264.7 Murine Macrophages." Infect. Immun. 77 (4): 1636-1648.

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19168747 | His6-SENP1CD

Stanisic, V., et al (2009) "OTU Domain-containing Ubiquitin Aldehyde-binding Protein 1 (OTUB1) Deubiquitinates Estrogen Receptor (ER)a and Affects ERa Transcriptional Activity." J. Biol. Chem. 284: 16135-16145 .

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19383985 | ubiquitin-aldehyde

Whitehurst, C.B., et al. (2009) "The Epstein-Barr Virus (EBV) Deubiquitinating Enzyme BPLF1 Reduces EBV Ribonucleotide Reductase Activity." J. Virol. 83: 4345-4353 .

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19244336 | Ub C-terminal hydrolase (UCH-L1)

Kim, A.H., et al. (2009) "A centrosomal Cdc20-APC pathway controls dendrite morphogenesis in postmitotic neurons.” Cell 136(2): 322–336.

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19167333 | ubiquitin aldehyde

Balastik, M., et al. (2008) "Deficiency in ubiquitin ligase TRIM2 causes accumulation of neurofilament light chain and neurodegeneration." PNAS 105 (33): 12016-12021.

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18687884 | ubiquitin aldehyde

Jalonen U, et al. (2008) "Compounds That Increase or Mimic Cyclic Adenosine Monophosphate Enhance Tristetraprolin Degradation in Lipopolysaccharide-Treated Murine J774 Macrophages." J. Pharm. Exp. Ther. 326: 514-522 .

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18469159 | ubiquitin aldehyde

Choi, E. et al. (2008) "Unique D Box and KEN Box Sequences Limit Ubiquitination of Acm1 and Promote Pseudosubstrate Inhibition of the Anaphase-promoting Complex." J. Biol. Chem. 283(35): 23701-23710.

http://www.jbc.org/content/283/35/23701.long | recombinant human ubiquitin

Kabuta T., et al. (2008). "Aberrant molecular properties shared by familial Parkinson’s disease-associated mutant UCH-L1 and carbonyl-modified UCH-L1." Hum. Mol. Genet. 17(10):1482-1496.

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18250096 | recombinant human UCH-L1

Le Negrate, G., et al. (2008) "Salmonella Secreted Factor L Deubiquitinase of Salmonella typhimurium Inhibits NF-κB, Suppresses IκB[alpha] Ubiquitination and Modulates Innate Immune Responses." J. Immunol. 180: 5045-56.

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18354230 | ubiquitin-aldehyde

Lu, K., et al. (2008) "Targeting WW domains linker of HECT-type ubiquitin ligase Smurf1 for activation by CKIP-1.“ Nature cell biology 10(8): 994–1002.

18641638 | HA–ubiquitin

Miyauchi, Y., et al. (2008) "The COP9/signalosome increases the efficiency of pVHL ubiquitin ligase-mediated hypoxia inducible factor-[alpha] ubiquitination." J. Biol. Chem. 283 (24):16622-16631.

| ubiquitin aldehyde

Newton, K., et al. (2008) "Ubiquitin Chain Editing Revealed by Polyubiquitin Linkage-Specific Antibodies.” Cell 134(4): 668–678.

18724939 | USP2CD

Ratia, K, et al. (2008). "A non-covalent class of papain-like protease/deubiquitinase inhibitors blocks SARS virus replication." PNAS 105 (42): 16119-16124 .

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18852458 | full-length USP-18, the catalytic domain of HAUSP, ubiquitin-AMC

Ogawa, M., et al. (2008) "Rines/RNF180, a novel RING finger gene-encoded product, is a membrane-bound ubiquitin ligase." Genes Cells 13: 397-409.

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18363970 | epoxomicin, lactacystin

Catic A., et al. (2007) "Screen for ISG15-cross-reactive Deubiquitinases." PLoS ONE 2(7): E769.

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17653289 | human recombinant USP14

Ghaboosi, N. and R. J. Deshaies (2007). "A Conditional Yeast E1 Mutant Blocks the Ubiquitin-Proteasome Pathway and Reveals a Role for Ubiquitin Conjugates in Targeting Rad23 to the Proteasome." Mol. Biol. Cell 18(5): 1953-1963.

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17360968 | ubiquitin-aldehyde

Hong, K. U., et al. (2007). "Functional Importance of the Anaphase-Promoting Complex-Cdh1-Mediated Degradation of TMAP/CKAP2 in Regulation of Spindle Function and Cytokinesis." Mol. Cell. Biol. 27(10): 3667-3681.

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17339342 | ubiquitin aldehyde

Kamynina, E., et al. (2007). "Regulation of Proto-Oncogenic Dbl by Chaperone-Controlled, Ubiquitin-Mediated Degradation.” Mol. Cell. Biol. 27(5): 1809-1822.

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17178836 | ubiquitin-aldehyde

Liu, Y., et al. (2007). "Histone H2AX Is a Mediator of Gastrointestinal Stromal Tumor Cell Apoptosis following Treatment with Imatinib Mesylate." Canc. Res. 67(6): 2685-2692 .

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17363589 | ubiquitin aldehyde

Rytkonen, A., et al. (2007) "SseL, a Salmonella deubiquitinase required for macrophage killing and virulence4." PNAS 104(9): 3502-3507.

| SENP1

Todi, S. V., et al. (2007). "Cellular turnover of the polyglutamine disease protein ataxin-3 is regulated by its catalytic activity." J. Biol. Chem.: 282(40): 29348-29358.

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17693639 | ubiquitin-aldehyde

Yi, Y.-J., et al. (2007). "Ubiquitin C-Terminal Hydrolase-Activity Is Involved in Sperm Acrosomal Function and Anti-Polyspermy Defense During Porcine Fertilization." Biol. Reprod.77:780-793.

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17671268 | recombinant human UCHL3

Avvakumov, G. V., et al. (2006). "Amino-terminal dimerization, NRDP1 (FLRF) - rhodanese interaction, and inhibited catalytic domain conformation of the ubiquitin specific protease 8 (USP8/UBPY)." J. Biol. Chem.281(49):38061-38070.

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17035239 | NEDD8-AMC

Das, C., et al. (2006). "Structural basis for conformational plasticity of the Parkinson's disease-associated ubiquitin hydrolase UCH-L1." PNAS 103(12): 4675-4680.

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16537382 | WT UCH-L1

Song, B.-L. and R. A. DeBose-Boyd (2006). "Insig-dependent Ubiquitination and Degradation of 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase Stimulated by δ- and γ-Tocotrienols." J. Biol. Chem. 281(35): 25054-25061.

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16831864 | ubiquitin-aldehyde

Yamada, M., J. Ohnishi, et al. (2006). "NARF, an emo-like Kinase (NLK)-associated Ring Finger Protein Regulates the Ubiquitylation and Degradation of T Cell Factor/Lymphoid Enhancer Factor (TCF/LEF)." J. Biol. Chem. 281(30): 20749-20760.

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16714285 | ubiquitin aldehyde

Zollars, E. S., et al. (2006). "Simple electrostatic model improves designed protein sequences." Protein Sci. 15(8): 2014-2018.

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http://onlinelibrary.wiley.com/doi/10.1110/ps.062105506/full | protease UCH-L3

Gentry M. S., et al. (2005) "From The Cover: Insights into Lafora disease: Malin is an E3 ubiquitin ligase that ubiquitinates and promotes the degradation of laforin." PNAS 102(24): 8501-8506.

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15930137 | WT ubiquitin

Koshikawa N. and Takenaga K. (2005) "Hypoxia-Regulated Expression of Attenuated Diphtheria Toxin A Fused with Hypoxia-Inducible Factor-1[alpha]/Oxygen-Dependent Degradation Domain Preferentially Induces Apoptosis of Hypoxic Cells in Solid Tumor." Can. Res. 65(2 .

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16357173 | ubiquitin aldehyde

Lindner H. A., et al. (2005) "The Papain-Like Protease from the Severe Acute Respiratory Syndrome Coronavirus is a Deubiquitinating Enzyme." J. Virol. 79(24): 15199-15208.

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16306591 | ubiquitin aldehyde

Lolkema M.P., et al. (2005) "Tumor Suppression by the von Hippel-Lindau Protein Requires Phosphorylation of the Acidic Domain." J. Biol. Chem. 280(23): 22205-22211.

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15824109 | ubiquitin aldehyde

Shirogane T., et al. (2005) "SCF[beta]-TRCP Controls Clock-dependent Transcription via Casein Kinase 1-dependent Degradation of the Mammalian Period-1 (Per1) Protein." J. Biol. Chem. 280(29): 26863-26872.

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15917222 | ubiquitin aldehyde

Zvonic S., et al. (2005) "Cross-talk among gp130 Cytokines in Adipocytes." J. Biol. Chem. 280(40): 33856-33863.

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16096272 | MG132

Burstein E., et al. (2004) "A novel role for XIAP in copper homeostasis through regulation of MURR1." EMBO J. 23(1): 244-254.

| ubiquitin aldehyde

Evans, P.C., et al. (2004). "Zinc-finger protein A20, a regulator of inflammation and cell survival has de-ubiquitination activity." Biochem. J. 378: 727-734.

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14748687 | [Lys63 ]polyubiquitin

Habelhah H., et al. (2004) "Ubiquitination and translocation of TRAF2 is required for activation of JNK but not of p38 or NF-κB." EMBO J. 23(2): 322-332.

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14713952 | Ub aldehyde

Nishikawa H., et al. (2004) "Mass Spectrometric and Mutational Analyses Reveal Lys-6-linked Polyubiquitin Chains Catalyzed by BRCA1-BARD1 Ubiquitin Ligase." J. Biol. Chem. 279(6): 3916-3924.

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14638690 | Ubal (ubiquitin aldehyde)

Song B.-L. and DeBose-Boyd R.A. (2004) "Ubiquitination of 3-Hydroxy-3-methylglutaryl-CoA Reductase in Permeabilized Cells Mediated by Cytosolic E1 and a Putative Membrane-bound Ubiquitin Ligase." J. Biol. Chem. 279(27): 28798-28806.

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15090540 | ubiquitin-aldehyde

Chang, W., et al. (2003) "TRF1 is degraded by ubiquitin-mediated proteolysis after release from telomeres." Genes & Dev. 17: 1328-1333.

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12782650 | methyl ubiquitin

Eguchi, H., et al. (2003). "Helicobacter pylori increases proteasome-mediated degradation of p27kip1 in gastric epithelial cells." Canc. Res. 63: 4739-4746.

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12907657 | ubiquitin aldehyde

Osaka, H., et al. (2003). "Ubiquitin carboxyl-terminal hydrolases L1 binds to and stabilizes monoubiquitin in neuron." Hum. Molec. Genet. 12: 1945-1958.

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12913066 | Ubiquitin-7-amido-4-methylcoumarin (Ub-AMC)

Sampathu, D.M., et al. (2003) "Ubiquitination of [alpha]-synuclein is not required for formation of pathological inclusions in [alpha]--Synucleinopathies." Am. J. Path. 163: 91-100.

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12819014 | ubiquitin-aldehyde, UCH-L1

Shim, M., et al. (2003). "Lithium stabilizes the CCAAT/Enhancer-binding protein [alpha] (C/EBP[alpha]) through a glycogen synthase kinase 3 (GSK3)-independent pathway involving direct inhibitor of proteasomal activity." Genes Cells 11(7): 731-744.

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12668682 | ubiquitin-aldehyde

Su, L., et al. (2003) "p190RhoGAP is cell-cycle regulated and affect cytokinesis." J. Cell. Biol. 163(3): 571-582 .

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14610059 | ubiquitin aldehyde