Product References - Buffers, solutions, standards

Boston Biochem's products have been used successfully in hundreds of UPP-related publications. We have listed our most recent references below to provide our customers with an exceptional resource for product use information. The references are organized to mirror our product divisions for easy location. Products referenced in each article are listed below in red. Please inquire to request archives as we have curated references back to 2003.

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23455478 | Mg-ATPb
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23345618 | 10x energy regeneration solution
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23362280 | ubiquitination reaction buffer
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23555887 | ATP-Mg2+
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23269275 | ATP
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23150437 | Mg2+-ATP
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23314748 | ATP
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23469016 | 20X energy regeneration mix
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23339190 | energy regeneration system
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23541081 | 10x ubiquitination buffer
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23212909 | energy regeneration solution

Zhang, S. et al. (2012) "GST:Identification of RNF8 as a ubiquitin ligase involved in targeting the p12 subunit of DNA polymerase δ for degradation in response to DNA damage." J. Biol. Chem.

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23233665 | ATP regenerating solution (ENS), ubiquitin aldehyde, HeLa S100 fraction, Recombinant human E1 ubiquitin activating enzyme, Recombinant human ubiquitin, Recombinant human ubiquitin K48R, Recombinant human ubiquitin K63R, Recombinant human UbcH5c, Recombinant human Cdc34, Recombinant human UbcH2

Guo, Z. et al. (2012) "Sequential Posttranslational Modifications Program FEN1 Degradation during Cell-Cycle Progression." Molecular cell.

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22749529 | ubiquitination reaction buffer, UBE1, UBE2M, HeLa S100 fraction, SUMOylation kits, ubiquitination kit

Zhao, Y. et al. (2012) "Non-Covalent Interaction between Polyubiquitin and GTP Cyclohydrolase 1 Dictates Its Degradation." PLoS ONE 7(9): e43306.

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22984419 | mixed ubiquitin chains (Ub2~7), sample buffer, Tetraubiquitin (Ub4), UIM-agarose

Zang, Y. et al. (2012) "Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage." Rice 5(1): 24.

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10.1186/1939-8433-5-24 | E1 enzyme, K48R ubiquitin, K63R ubiquitin, reaction buffer, Ub, Ubc13, Uev1A, ubiquitin-AMC, nonbiotinylated USP2 catalytic domain, USP10, USP47, USP5, USP7

Merkx, R. et al. (2012) "Synthesis and Evaluation of a Selective Fluorogenic Pup Derived Assay Reagent for Dop, a Potential Drug Target in Mycobacterium tuberculosis." Chembiochem 13(14): 2056–2060.

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22927162 | energy regeneration solution

Humphrey, R.K. et al. (2012) "Lysine 63-linked Ubiquitination Modulates Mixed Lineage Kinase-3 Interaction with JIP1 Scaffold protein in Cytokine-induced Pancreatic Beta Cell Death." J. Biol. Chem.

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23172226 | Mg-ATP mix cofactor mix, Myc-ubiquitin, recombinant UbE1,UbeH13-Uev1a heterodimer complex, Ub conjugation buffer,

Henle, A.M. et al. (2012) "Enzymatic Discovery of a HER-2/neu Epitope That Generates Cross-Reactive T Cells." J. Immunol.

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23180824 | Suc-LLVY-AMC, synthetic lactacystin, reaction buffer (25 mM HEPES, 0.5 mM EDTA, pH 7.6), immunoproteasome, PA28 activator alpha subunit, purified human 20S proteasome

Marfella, R. et al. (2012) "Dipeptidyl Peptidase 4 Inhibition May Facilitate Healing of Chronic Foot Ulcers in Patients with Type 2 Diabetes." Experimental Diabetes Research 2012: 1–11.

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23197976 | SDS-activation kit

Huett, A. et al. (2012) "The LRR and RING Domain Protein LRSAM1 Is an E3 Ligase Crucial for Ubiquitin-Dependent Autophagy of Intracellular Salmonella Typhimurium." Cell Host Microbe 12(6): 778–790.

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23245322 | ubiquitination buffer, HA-tagged free ubiquitin, His-tagged free ubiquitin

Kensche, T. et al. (2012) "Analysis of Nuclear Factor-κB (NF-κB) Essential Modulator (NEMO) Binding to Linear and Lysine-linked Ubiquitin Chains and Its Role in the Activation of NF-κB." J. Biol. Chem. 287(28): 23626–23634.

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22605335 | creatine phosphokinase, HeLa-S-100 fraction II, ubiquitin, ubiquitin aldehyde

Sambuughin, N. et al. (2012) "KBTBD13 interacts with Cullin 3 to form a functional ubiquitin ligase." Biochemical and biophysical research communications. 2012 Apr 20;

22542517 | ubiquitin reaction buffer

Luo, K. et al. (2012) "Sumoylation of MDC1 is important for proper DNA damage response." The EMBO journal.  2012 May 25;

22635276 | E1 stop buffer

Bell, J.L. et al. (2012) "TRIM16 Acts as an E3 Ubiquitin Ligase and Can Heterodimerize with Other TRIM Family Members." PLoS ONE 7(5): e37470.

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22629402 | In Vitro Ubiquitination Buffer containing E1 and E2

Luo, K. et al. (2012) "Sumoylation of MDC1 is important for proper DNA damage response." The EMBO journal.  2012 May 25;

22635276 | 10 x reaction buffer

Shabbeer, S. et al. (2012) "BRCA1 targets G2/M cell cycle proteins for ubiquitination and proteasomal degradation." Oncogene.

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23246971 | recombinant His-cyclin B, HA-ubiquitin, E1, UbcH5c, UbcH1, Ubc13-MMS2, UbcH10, ATP (ERS), His-BRCA1, His-BARD1

Li, C., et al. (2011) "Tumor-suppressor role for the SPOP ubiquitin ligase in signal-dependent proteolysis of the oncogenic co-activator SRC-3/AIB1.” Oncogene 30(42): 4350–4364.

21577200 | ATP

Zhang, X., et al. (2011) "USP4 inhibits p53 through deubiquitinating and stabilizing ARF-BP1.” The EMBO Journal 30(11): 2177–2189.

21522127 | in vitro ubiquitination reaction buffer / Mg-ATP / stop buffer

James, T., et al. (2011) “Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease.” PNAS, 108: 2222-2227.

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21245344 | AMC standard

Zhu, F., et al. (2011) “Phosphorylation of H2AX at Ser139 and a New Phosphorylation Site Ser16 by RSK2 Decreases H2AX Ubiquitination and Inhibits Cell Transformation.” Cancer Research, 71: 393.

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21224359 | energy regeneration solution (ERS) buffer

Cao, X., et al. (2011) “WW Domain-containing E3 Ubiquitin Protein Ligase 1 (WWP1) Delays Cellular Senescence by Promoting p27 Kip1 Degradation in Human Diploid Fibroblasts.” J. Biol. Chem. 286: 33447 - 33456.

21795702 | reaction buffer

Cosper, P. and Leinwand, L. (2011) “Cancer Causes Cardiac Atrophy and Autophagy in a Sexually Dimorphic Manner.” Cancer Research, 71: 1710.

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21163868 | Energy Regeneration Solution

Galisson, F., et al. (2011) “A Novel Proteomics Approach to Identify SUMOylated Proteins and Their Modification Sites in Human Cells.” Molecular and Cellular Proteomics, 10: M110.004796.

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21098080 | Mg-ATP

Kim, J., et al (2011) “Role of TRIM5[alpha] RING Domain E3 Ubiquitin Ligase Activity in Capsid Disassembly, Reverse Transcription Blockade, and Restriction of Simian Immunodeficiency Virus.” J. Virol. 85: 8116 - 8132.

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21680520 | ATP / Energy regeneration system

Mao, X., et al. (2011) “COMMD1 (Copper Metabolism MURR1 Domain-containing Protein 1) Regulates Cullin RING Ligases by Preventing CAND1 (Cullin-associated Nedd8-dissociated Protein 1) Binding.” J. Biol. Chem. 286: 32355 - 32365.

21778237 | ATP regenerating buffer

Scortegagna, M., et al. (2011) “USP13 Enzyme Regulates Siah2 Ligase Stability and Activity via Noncatalytic Ubiquitin-binding Domains.” J. Biol. Chem. 286: 27333 - 27341.

21659512 | ATP

Uchida, S., et al. (2011) “SCF[beta]TrCP mediates stress-activated MAPK-induced Cdc25B degradation.” J. Cell Sci. 124: 2816 - 2825.

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21807946 | ATP-regenerating system

Cui, Y., et al. (2011) "SCFFBXL15 regulates BMP signalling by directing the degradation of HECT-type ubiquitin ligase Smurf1.” The EMBO Journal 30(13): 2675–2689.

21572392 | ubiquitination assay buffer

Gerlach, B., et al. (2011) "Linear ubiquitination prevents inflammation and regulates immune signalling.” Nature 471(7340): 591–596.

| ERS (energy regeneration system?)

Kassmeier, M.D., et al. (2011) "VprBP binds full-length RAG1 and is required for B-cell development and V (D) J recombination fidelity.” The EMBO Journal. 31(4):945-58.

| reconjugation buffer

Tang, L.Y., et al. (2011) "Ablation of Smurf2 reveals an inhibition in TGF-[beta] signalling through multiple mono-ubiquitination of Smad3.” The EMBO Journal. 30(23):4777-89.

22045334 | ATP

Banerjee, S., et al. (2010) “Modulation of SCF[beta]-TrCP-dependent IB Ubiquitination by Hydrogen Peroxide.” J. Biol. Chem. 285: 2665 - 2675.

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19933270 | Mg-ATP

Ensminger, A. and Isberg, R. (2010) “E3 ubiquitin ligase activity and targeting of BAT3 by multiple Legionella pneumophila translocated substrates.” Infect. Immun., 78: 3905 - 3919.

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20547746 | Energy regenerating solution

Hallows, K.R., et al. (2010) “Phosphopeptide Screen Uncovers Novel Phosphorylation Sites of Nedd4-2 That Potentiate Its Inhibition of the Epithelial Na+ Channel.” J. Biol. Chem. 285: 21671 - 21678.

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20466724 | ATP

Sánchez Manchinelly, S.A. (2010) “Mitotic Down-regulation of p190RhoGAP Is Required for the Successful Completion of Cytokinesis.” J. Biol. Chem. 285: 26923 - 26932.

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20534586 | Energy-Regenerating System

Song, I. Y., et al. (2010) “Rad18-mediated Translesion Synthesis of Bulky DNA Adducts Is Coupled to Activation of the Fanconi Anemia DNA Repair Pathway.” J. Biol. Chem. 285: 31525-31536.

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20675655 | ATP-regenerating system

Chu, Y. and Yang, X. (2010) "SUMO E3 ligase activity of TRIM proteins.” Oncogene 30(9): 1108–1116.

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20972456 | ATP

Fujita, K., et al. (2010) "Positive feedback between p53 and TRF2 during telomere-damage signalling and cellular senescence.” Nature cell biology 12(12): 1205–1212.

21057505 | energy solution / stop buffer

Liu, J. and Ma, J. (2010) "Fates-shifted is an F-box protein that targets Bicoid for degradation and regulates developmental fate determination in Drosophila embryos.” Nature cell biology 13(1): 22–29.

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21170036 | Mg–ATP

Burns, K.E., et al. (2009) "Proteasomal Protein Degradation in Mycobacteria Is Dependent upon a Prokaryotic Ubiquitin-like Protein." J. Biol. Chem. 284(5): 3069-3075.

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19028679 | energy regeneration solution

Christopher, B., et al. (2009) “The EBV Deubiquitinating Enzyme, BPLF1, Reduces EBV Ribonucleotide Reductase Activity.” J. Virol. JVI.02195-08v1 .

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19244336 | reaction mixture buffer (50 mM Tris, pH 7.5, 2 mM Mg-ATP)

Maerki, S., et al. (2009) “The Cul3–KLHL21 E3 ubiquitin ligase targets Aurora B to midzone microtubules in anaphase and is required for cytokinesis.” J. Cell Biol. 187: 791 - 800.

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19995937 | Mg-ATP / ubiquitination reaction buffer

Mao, X,. et al. (2009) "GCN5 is a required cofactor for a ubiquitin ligase that targets NF-kB/RelA." Genes & Dev. 23: 849-861.

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19339690 | ATP regenerating buffer

Nakao, R., et al. (2009) "Ubiquitin ligase Cbl-b is a negative regulator for IGF-1 signaling during muscle atrophy caused by unloading." Mol. Cell. Biol.: MCB.01347-08.

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19546233 | reaction buffer containing an ATP-regenerating system

Stanisic, V., et al (2009) "OTU Domain-containing Ubiquitin Aldehyde-binding Protein 1 (OTUB1) Deubiquitinates Estrogen Receptor (ER)a and Affects ERa Transcriptional Activity." J. Biol. Chem. 284: 16135-16145 .

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19383985 | energy solution

Whitehurst, C.B., et al. (2009) "The Epstein-Barr Virus (EBV) Deubiquitinating Enzyme BPLF1 Reduces EBV Ribonucleotide Reductase Activity." J. Virol. 83: 4345-4353 .

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19244336 | Mg-ATP

Bekker-Jensen, S., et al. (2009) "HERC2 coordinates ubiquitin-dependent assembly of DNA repair factors on damaged chromosomes.” Nature cell biology 12(1): 80–86.

20023648 | ubiquitylation reaction buffer

Jin, Z., et al. (2009) "Cullin3-Based Polyubiquitination and p62-Dependent Aggregation of Caspase-8 Mediate Extrinsic Apoptosis Signaling.” Cell 137(4): 721–735.

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19427028 | Mg-ATP solution / Ubiquitin Conjugation Reaction Buffer

Brooks, W.S., et al. (2008) "G2E3 Is a Dual Function Ubiquitin Ligase Required for Early Embryonic Development." J. Biol. Chem. 283(32): 22304-22315.

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18511420 | Energy Regeneration System

Daulny, A., et al. (2008) "Modulation of RNA polymerase II subunit composition by ubiquitylation." PNAS 105(50): 19649-19654.

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19064926 | Energy Regeneration Solution

Karagianni, P., et al. (2008) "ICBP90, a Novel Methyl K9 H3 Binding Protein Linking Protein Ubiquitination with Heterochromatin Formation." Mol. Cell. Biol. 28: 705-17.

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http://mcb.asm.org/content/28/2/705.long | ATP

Liu, J., et al. (2008) "A therapeutic dose of doxorubicin activates ubiquitin-proteasome system-mediated proteolysis by acting on both the ubiquitination apparatus and proteasome." Am. J. Physiol. Heart.Circ. Physiol. 295(6): H2541-H2550.

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18978187 | energy regenerating solution (B-10)

Malakhova O.A. and Zhang D-E. (2008) "ISG15 Inhibits Nedd4 Ubiquitin E3 Activity and Enhances the Innate Antiviral Response." J. Biol. Chem. 283: 8783-8787.

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18287095 | ATP energy regeneration system

Sekine K., et al. (2008). "Small Molecules Destabilize cIAP1 by Activating Auto-ubiquitylation." J. Biol. Chem. 283(14): 8961-8968.

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18230607 | Mg-ATP

Lu, K., et al. (2008) "Targeting WW domains linker of HECT-type ubiquitin ligase Smurf1 for activation by CKIP-1.“ Nature cell biology 10(8): 994–1002.

18641638 | ubiquitylation assay buffer

Chu, I., et al. (2007). "Src promotes estrogen-dependent estrogen receptor [alpha] proteolysis in human breast cancer." J. Clin. Invest. 117(8): 2205-2215.

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http://www.jci.org/articles/view/21739 | energy regenerating solution

Hong, K. U., et al. (2007). "Functional Importance of the Anaphase-Promoting Complex-Cdh1-Mediated Degradation of TMAP/CKAP2 in Regulation of Spindle Function and Cytokinesis." Mol. Cell. Biol. 27(10): 3667-3681.

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17339342 | energy regeneration solution

Kamynina, E., et al. (2007). "Regulation of Proto-Oncogenic Dbl by Chaperone-Controlled, Ubiquitin-Mediated Degradation.” Mol. Cell. Biol. 27(5): 1809-1822.

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17178836 | ATP regeneration solution

Skaar, J. R., et al. (2007). "PARC and CUL7 Form Atypical Cullin RING Ligase Complexes.” Cancer Res. 67(5): 2006-2014.

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17332328 | Mg-ATP

Sun, Z., et al. (2007). "Keap1 Controls Post-Induction Repression of the Nrf2-Mediated Antioxidant Response by Escorting Nuclear Export of Nrf2." Mol. Cell. Biol.: 27(18):6334-6349.

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17636022 | ATP

Todi, S. V., et al. (2007). "Cellular turnover of the polyglutamine disease protein ataxin-3 is regulated by its catalytic activity." J. Biol. Chem.: 282(40): 29348-29358.

| ATP regeneration system

Dai, M.-S., D. et al. (2006). "Regulation of the MDM2-p53 Pathway by Ribosomal Protein L11 Involves a Post-ubiquitination Mechanism." J. Biol. Chem. 281(34): 24304-24313.

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16803902 | Mg-ATP

Havens, C. G., et al. (2006) "Regulation of Late G1/S Phase Transition and APCCdh1 by Reactive Oxygen Species." Mol. Cell. Biol. 26(12): 4701-4711.

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16738333 | energy regeneration system

Sangadala, S., et al. (2006). "LIM Mineralization Protein-1 Potentiates Bone Morphogenetic Protein Responsiveness via a Novel Interaction with Smurf1 Resulting in Decreased Ubiquitination of Smads." J. Biol. Chem. 281(25): 17212-17219.

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16611643 | ATP / creatine kinase-ATP generating system

Wada K. And Kamitani T. (2006) "UnpEL/Usp4 is ubiquitinated by Ro52 and deubiquitinated by itself." Biochem. Biophys. Res. Comm. 342(1): 253-258.

16472766 | MgATP

Sparks, S. E., et al. (2005) "Use of a cell-free system to determine UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities in human hereditary inclusion body myopathy." Glycobiology 15(11): 1102-1110.

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15987957 | ManNAc, MgCl2, and Mg‐ATP

Adachi K., et al. (2004) "Ubiquitylation of Nascent Globin Chains in a Cell-free System." J. Biol. Chem. 279(40): 41767-41774.

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15297454 | energy regenerating source (ERS)

Wei, W., et al.(2004) "Degradation of the SCF component Skp2 in cell-cycle phase G1 by the anaphase promoting complex." Nature 428: 194-198.

15014503 | ATP-regenerating system

Chang, W., et al. (2003) "TRF1 is degraded by ubiquitin-mediated proteolysis after release from telomeres." Genes & Dev. 17: 1328-1333.

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12782650 | energy solution

Sampathu, D.M., et al. (2003) "Ubiquitination of [alpha]-synuclein is not required for formation of pathological inclusions in [alpha]--Synucleinopathies." Am. J. Path. 163: 91-100.

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12819014 | energy-regenerating system

Su, L., et al. (2003) "p190RhoGAP is cell-cycle regulated and affect cytokinesis." J. Cell. Biol. 163(3): 571-582 .

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14610059 | energy regenerating system

Shim, M., et al. (2003). "Lithium stabilizes the CCAAT/Enhancer-binding protein [alpha] (C/EBP[alpha]) through a glycogen synthase kinase 3 (GSK3)-independent pathway involving direct inhibitor of proteasomal activity." Genes Cells 11(7): 731-744.

| energy regeneration system (ERS)