Product References

Boston Biochem's products have been used successfully in hundreds of UPP-related publications. We have listed our most recent references below to provide our customers with an exceptional resource for product use information. The references are organized to mirror our product divisions for easy location. Products referenced in each article are listed below in red. Please inquire to request archives as we have curated references back to 2003.

E1 Activating

Hebron, M.L. et al. (2012) "Parkin ubiquitinates Tar-DNA binding protein-43 (TDP-43) and promotes its cytosolic accumulation via interaction with histone deacetylase 6 (HDAC6)." J. Biol. Chem.

23258539 | recombinant human ubiquitin, recombinant UbcH7, E1 recombinant enzyme,
23453969 | recombinant E1 ubiquitin activating enzyme
23319590 | E1
23319590 | E1
23269275 | E1
23459942 | E1
23209297 | recombinant human Uba1
23097446 | SUMO-activating E1 (SAE1-SAE2)
23345618 | ubiquitin activating enzyme (Ube1)
23287719 | E1 (Ube1)
23379478 | E1
23332158 | “Ubiquitination assays were performed using enzymes obtained from Boston Biochem”
23362280 | E1 (UBE1)
23555887 | E1
23463011 | UBE1
23463011 | UBE1
23486064 | E1
23150437 | E1 enzyme
23150407 | recombinant rabbit E1 enzyme
23314748 | E1
23359647 | E1 enzyme
23418353 | E1 from yeast
23460917 | human E1
23300442 | UBE1
23453970 | recombinant human E1

Inhibitors

23454125 | MG132

Rinetti, G.V. and Schweizer, F. (2010) “Ubiquitination Acutely Regulates Presynaptic Neurotransmitter Release in Mammalian Neurons.” J. Neurosci. 30: 3157 - 3166.

20203175 | clasto-lactacystin [beta]-lactone

E2 Conjugating

Hebron, M.L. et al. (2012) "Parkin ubiquitinates Tar-DNA binding protein-43 (TDP-43) and promotes its cytosolic accumulation via interaction with histone deacetylase 6 (HDAC6)." J. Biol. Chem.

23258539 | recombinant human ubiquitin, recombinant UbcH7, E1 recombinant enzyme,
23328397 | UbcH5c
23453969 | Ubc13 E2 enzyme
23455478 | rabbit E1 (UBE1)
23455478 | rabbit E2 (UbcH5b)
23459942 | UbcH5b
23287719 | E2 (Ubch7)
23401859 | GST-UBC12
23332158 | “Ubiquitination assays were performed using enzymes obtained from Boston Biochem”
23362280 | UbcH5b
23555887 | E2
23463011 | Rad6B
23486064 | Ubch3
23486064 | Ubch5c
23269275 | E2 (Ubc5a)
23150437 | E2 enzyme (UbcH5a)
23150407 | recombinant human E2 enzyme UbcH5c
23314748 | UbcH5
23418353 | UbcH13/Uev1a
23418353 | UbcH5b
23453970 | recombinant human UbcH5a/UBE2D1
23453805 | hRAD6B
23453805 | hUbcH13
23453805 | hUbcH5c
23212909 | UbcH5b

E3 Ligase

23512198 | his-purified recombinant Cullin 1
23512198 | his-purified recombinant Rbx1
23512198 | his-purified recombinant Skp1

Cotto-Rios, X.M. et al. (2012) "Deubiquitinases as a signaling target of oxidative stress." Cell Rep 2(6): 1475–1484.

23219552 | UbVME, recombinant ubiquitin chain K48-linked, UCH-L1, SENP1-CD, STAM, recombinant ubiquitin chain K63-linked, SUMO1-vs, USP7, AMSH,

Hou, X. et al. (2012) "Mining and characterization of ubiquitin E3 ligases expressed in the mouse testis." BMC Genomics 13: 495.

22992278 | MYC-ubiquitin, purified recombinant E2 (UBE2D2/UBCH5B), purified recombinant E2 (UBE2D3/UBCH5C), purified recombinant E2 (Ube2k/E2-25K), purified recombinant E2 (UBE2L3/ UBCH7), putative E3s with GST tag, yeast E1

Burchell, L. et al. (2012) "Small, N-terminal tags activate parkin e3 ubiquitin ligase activity by disrupting its autoinhibited conformation." PLoS ONE 7(4): e34748.

22496854 | His-Parkin

Johnson, B.N. et al. (2012) "The ubiquitin E3 ligase parkin regulates the proapoptotic function of Bax." Proc. Natl. Acad. Sci. U.S.A. 109(16): 6283–6288.

22460798 | UbcH7

Schneider, S. et al. (2012) "Development of a homogeneous AlphaLISA ubiquitination assay using ubiquitin binding matrices as universal components for the detection of ubiquitinated proteins." Biochimica et biophysica acta. 2012 Apr 5.

22504171

Johnson, B.N. et al. (2012) "The ubiquitin E3 ligase parkin regulates the proapoptotic function of Bax." Proc. Natl. Acad. Sci. U.S.A. 109(16): 6283–6288.

22460798

Kalia, L.V., et al. (2011) “Ubiquitinylation of [alpha]-Synuclein by Carboxyl Terminus Hsp70-Interacting Protein (CHIP) Is Regulated by Bcl-2-Associated Athanogene 5 (BAG5).” PloS one, 6: e14695. doi:10.1371/journal.pone.0014695 .

21358815 | HDM2, MuRF1

Bosanac, I., et al. (2011) “Modulation of K11-Linkage Formation by Variable Loop Residues within UbcH5A.” Journal of Mol. Bio.: doi:10.1016/j.jmb.2011.03.011.* .

21396940 | MURF1

Shin, J., et al. (2011) “PARIS (ZNF746) Repression of PGC-1[alpha] Contributes to Neurodegeneration in Parkinson's Disease.” Cell, 144: 689-702.

21376232 | His-Parkin

Jin, X, et al. (2010) “An Atypical E3 Ligase Zinc Finger Protein 91 Stabilizes and Activates NF-B-inducing Kinase via Lys63-linked Ubiquitination.” J. Biol. Chem. 285: 30539-30547.

20682767 | Lys63-only ubiquitin

Kaneko, M., et al. (2010) “Loss of HRD1-Mediated Protein Degradation Causes Amyloid Precursor Protein Accumulation and Amyloid-[beta] Generation.” J. Neurosci., 3924 - 3932.

20237263 | RP-HRD1 (E3)

Chymkowitch, P., et al. (2010) "The phosphorylation of the androgen receptor by TFIIH directs the ubiquitin/proteasome process.” The EMBO Journal 30(3): 468–479.

21157430 | MDM2

Fu, W., et al. (2009) "MDM2 Acts Downstream of p53 as an E3 Ligase to Promote FOXO Ubiquitination and Degradation." J. Biol. Chem. 284: 13987-14000.

19321440 | MDM2

Siepe, D. and Jentsch, S. (2009) "Prolyl isomerase Pin1 acts as a switch to control the degree of substrate ubiquitylation.” Nature Cell Biology 11(8): 967–972.

19597489 | recombinant E3 (His6-Mdm2)

Newton, K., et al. (2008) "Ubiquitin Chain Editing Revealed by Polyubiquitin Linkage-Specific Antibodies.” Cell 134(4): 668–678.

18724939 | MuRF1 autoubiquitination reactions

Hrecka, K., M. Gierszewska, et al. (2007). "Lentiviral Vpr usurps Cul4-DDB1[VprBP] E3 ubiquitin ligase to modulate cell cycle." PNAS 104(28): 11778-11783.

17609381

Fortier, J. M. and J. Kornbluth (2006). "NK Lytic-Associated Molecule, Involved in NK Cytotoxic Function, Is an E3 Ligase." J. Immunol. 176(11): 6454-6463.

Polyclonal UbcH8 Ab

Dai, M.-S., D. et al. (2006). "Regulation of the MDM2-p53 Pathway by Ribosomal Protein L11 Involves a Post-ubiquitination Mechanism." J. Biol. Chem. 281(34): 24304-24313.

16803902 | purified human recombinant E2 (UbcH5a)

Burstein E., et al. (2004) "A novel role for XIAP in copper homeostasis through regulation of MURR1." EMBO J. 23(1): 244-254.

14685266 | E3

Deconjugating

23536702 | His6-USP2CD (UBP41 catalytic domain)
23375434 | human recombinant isopeptidase T

Cotto-Rios, X.M. et al. (2012) "Deubiquitinases as a signaling target of oxidative stress." Cell Rep 2(6): 1475–1484.

23219552 | UbVME, recombinant ubiquitin chain K48-linked, UCH-L1, SENP1-CD, STAM, recombinant ubiquitin chain K63-linked, SUMO1-vs, USP7, AMSH,

Zang, Y. et al. (2012) "Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage." Rice 5(1): 24.

10.1186/1939-8433-5-24 | E1 enzyme, K48R ubiquitin, K63R ubiquitin, reaction buffer, Ub, Ubc13, Uev1A, ubiquitin-AMC, nonbiotinylated USP2 catalytic domain, USP10, USP47, USP5, USP7

Reverdy, C. et al. (2012) "Discovery of Specific Inhibitors of Human USP7/HAUSP Deubiquitinating Enzyme." Chem. Biol. 19(4): 467–477.

22520753 | UCH-L1, UCH-L3, USP2, USP20, USP5, E-340, E-325, E-506, E-344, E-322

Issaenko, O.A. and Amerik, A.Y. (2012) "Chalcone-based small-molecule inhibitors attenuate malignant phenotype via targeting deubiquitinating enzymes." Cell cycle (Georgetown, Tex.) 11(9).

22510564 | purified human DUB, purified USP5

Shi, T. et al. (2012) "Identification Of Small Molecule TRABID Deubiquitinase Inhibitors By Computation-Based Virtual Screen." BMC chemical biology 12(1): 4.

22584113 | A20 catalytic domain

Capodagli, G., et al. (2011) “Structural analysis of a viral ovarian tumor domain protease from the Crimean-Congo Hemorrhagic Fever virus in complex with covalently bonded ubiquitin.” Journal of Virology, 85: 3621-3630.

21228232 | Ub-AMC

Chen, B.B., et al (2011) “Calmodulin antagonizes a calcium-activated SCF ubiquitin E3 ligase subunit, FBXL2, to regulate surfactant homeostasis.” Molecular and Cellular Biology: doi:10.1128/MCB.00723-10.” 

21343341 | ubiquitin aldehyde

James, T., et al. (2011) “Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease.” PNAS, 108: 2222-2227.

21245344 | ISG15–AMC

Cao, X., et al. (2011) “WW Domain-containing E3 Ubiquitin Protein Ligase 1 (WWP1) Delays Cellular Senescence by Promoting p27 Kip1 Degradation in Human Diploid Fibroblasts.” J. Biol. Chem. 286: 33447 - 33456.

21795702 | Wild-type ubiquitin

Cosper, P. and Leinwand, L. (2011) “Cancer Causes Cardiac Atrophy and Autophagy in a Sexually Dimorphic Manner.” Cancer Research, 71: 1710.

21163868 | ubiquitin aldehyde

Cotto-Rios, X.M., et al. (2011) “APC/CCdh1-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage.” J. Cell Biol. 194: 177 - 186.

21768287 | HA-UbVS probe

Huang, Z., et al. (2011) “Deubiquitylase HAUSP stabilizes REST and promotes maintenance of neural progenitor cells.” Nature Cell Biology, 13: 142-152.

21258371 | ubiquitin aldehyde

Lu, J., et al. (2011) “RNF170 Protein, an Endoplasmic Reticulum Membrane Ubiquitin Ligase, Mediates Inositol 1,4,5-Trisphosphate Receptor Ubiquitination and Degradation.” J. Biol. Chem. 286: 24426 - 24433.

21610068 | HA-ubiquitin

Lu, K., et al. (2011) “Pivotal role of the SMURF1 C2 domain in substrate selection.” The Journal of Biological Chemistry: doi:10.1074/jbc.M110.211979.

21402695 | HA–ubiquitin

Stringer, D. and Piper, R. (2011) “A single ubiquitin is sufficient for cargo protein entry into MVBs in the absence of ESCRT Ubiquitination.” JCB, 192: 229-242.

21242292 | Ub-Aldehyde

Uchida, S., et al. (2011) “SCF[beta]TrCP mediates stress-activated MAPK-induced Cdc25B degradation.” J. Cell Sci. 124: 2816 - 2825.

21807946 | ubiquitin-aldehyde

Wickliffe, K., et al. (2011) “The Mechanism of Linkage-Specific Ubiquitin Chain Elongation by a Single-Subunit E2.” Cell, 144: 769-781.

21376237 | wild-type ubiquitin

Yang, K., et al. (2011) “Regulation of the Fanconi anemia pathway by a SUMO-like delivery network.” Genes & Dev. 25: 1847 - 1858.

21896657 | Ub-AMC

Mansfeld, J., et al. (2011) "APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.” Nature cell biology 13(10): 1234–1243.

21926987 | anti-UbcH10

Chenon, M., et al. (2011) "A viral deubiquitylating enzyme targets viral RNA-dependent RNA polymerase and affects viral infectivity.” The EMBO Journal 31(3): 741–753.

22117220 | Ub-AMC

Jones, M.J.K., et al. (2011) "Dysregulation of DNA polymerase [kappa] recruitment to replication forks results in genomic instability.” The EMBO Journal. 31(4):908-18.

recombinant USP2 catalytic domain (DUB-CD)

Artavanis-Tsakonas, K., et al. (2010) “Characterization and Structural Studies of the Plasmodium falciparum Ubiquitin and Nedd8 Hydrolase UCHL3.” J. Biol. Chem. 285: 6857 - 6866.

20042598 | Nedd8-AMC

Clapp, K. M., et al. (2010) “C331A Mutant of Neuronal Nitric-oxide Synthase Is Labilized for Hsp70/CHIP (C Terminus of HSC70-interacting Protein)-dependent Ubiquitination.” J. Biol. Chem. 285: 33642-33651.

20729196 | ubiquitin aldehyde

Proteasome

23540790 | purified human constitutive 20S proteasome
23318177 | 26S proteosomes
23318177 | purified recombinant 19S RP
23150437 | purified human 26S proteasomes
23302496 | 20S rabbit proteosome
23393593 | inhibitor LC

Gobec, M. et al. (2012) "Selective Cytotoxicity of Amidinopiperidine Based Compounds Towards Burkitt’s Lymphoma Cells Involves Proteasome Inhibition." PLoS ONE 7(7): e41961.

22860040 | purified human 20S proteasome

Zhu, Y. et al. (2012) "Synthesis and inhibitory activities against colon cancer cell growth and proteasome of alkylresorcinols." J. Agric. Food Chem. 60(35): 8624–8631.

22897570 | human 20S proteasome, proteasome activator PA28, Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY-AMC)

Zhao, X. et al. (2012) "Orai1 mediates exacerbated ca(2+) entry in dystrophic skeletal muscle." PLoS ONE 7(11): e49862.

23185465 | lactacystin

Zhang, Z. et al. (2012 "1,10-Phenanthroline promotes copper complexes into tumor cells and induces apoptosis by inhibiting the proteasome activity." J. Biol. Inorg. Chem. 17(8): 1257–1267.

23053530 | purified human 20S proteasome, human ubiquitin activating enzyme E1, UbcH10

Xu, X. et al. (2012) "Identification of insulin receptor substrate 1’s degradation determinants for signaling cullin-RING E3 ubiquitin ligase 7-mediated ubiquitination." J. Biol. Chem. 287(48):40758-66.

23045529 | E1, MG132

Blackburn C, Barrett C, Blank JL, et al. "Optimization of a series of dipeptides with a P3 β-neopentyl asparagine residue as non-covalent inhibitors of the chymotrypsin-like activity of human 20S proteasome." Med. Chem. Commun. 2012 May 30;3(6):710–719. 

10.1039/C2MD20060K | purified human erythrocyte and peripheral blood monocyte 20S proteasomes, recombinant proteasomal activator PA28alpha, Z-LLE-AMC

Held, S.A.E. et al. (2012) "Imatinib mesylate and nilotinib affect MHC-class I presentation by modulating the proteasomal processing of antigenic peptides." Cancer Immunol. Immunother.

23184338 | isolated and purified 20S proteasomes

Lu, B. et al. (2012) "Phosphorylation of Human TFAM in Mitochondria Impairs DNA Binding and Promotes Degradation by the AAA(+) Lon Protease." Mol. Cell.

23201127 | 20S proteasome

Henle, A.M. et al. (2012) "Enzymatic Discovery of a HER-2/neu Epitope That Generates Cross-Reactive T Cells." J. Immunol.

23180824 | Suc-LLVY-AMC, synthetic lactacystin, reaction buffer (25 mM HEPES, 0.5 mM EDTA, pH 7.6), immunoproteasome, PA28 activator alpha subunit, purified human 20S proteasome

Guan, B. et al. (2012) "Functional Analysis of In-frame Indel ARID1A Mutations Reveals New Regulatory Mechanisms of Its Tumor Suppressor Functions." Neoplasia 14(10): 986–993.

23097632 | MG132

Dechavanne, V. et al. (2012) "Purification and separation of the 20S immunoproteasome from the constitutive proteasome and identification of the subunits by LC-MS." Protein Expr. Purif.

23147206 | Rabbit AP-123 polyclonal antibody, anti-beta2-20S human proteasome

Burrows, A.C. et al. (2012) "Skp1-Cul1-F-box Ubiquitin Ligase (SCFβTrCP)-Mediated Destruction of the Ubiquitin-Specific Protease USP37 During G2-phase Promotes Mitotic Entry." J. Biol. Chem.

23027877 | MG132, UbcH10 antibody

Foy, R.L. et al. (2012) "Polycystin-1 regulates the stability and ubiquitination of transcription factor Jade-1." Hum. Mol. Genet.

23001567 | lactacystin, MG132

Carvalho, A.N. et al. (2012) "Ubiquitin-Proteasome System Impairment and MPTP-Induced Oxidative Stress in the Brain of C57BL/6 Wild-type and GSTP Knockout Mice." Mol. Neurobiol.

23129554 | Z-leucine-leucine-glutamic acid-7-amido-4-methylcoumarin (Z-LLE-AMC), Boc-leucine-arginine-arginine-7-amido-4-methylcoumarin (Boc-LRR-AMC), succcinyl-leucine-leucine-valine-tyro-sine-7-amido-4-methylcoumarin (Suc-LLVY-AMC), isopeptidase inhibitor (Ubiquitin aldehyde [Ubal]), MG132,

Ismail, A. et al. (2012) "Proteasome Inhibitory Potential of Commonly Consumed Dietary Ingredients." International Journal of Food and Nutrition Science 1(4): 27–31.

20S proteasome purified from rabbit

Lansdell, T.A. et al. (2012) "Non-competitive modulation of the proteasome by imidazoline scaffolds overcome bortezomib resistant and delay MM tumor growth in vivo." ACS Chem. Biol.

23198928 | purified proteasome particles, Suc-LLVY-AMC, Z-ARR-AMC, Z-LLE-AMC

Blount, J.R. et al. (2012) "Ubiquitin-Specific Protease 25 Functions in Endoplasmic Reticulum-Associated Degradation." PLoS ONE 7(5): e36542.

22590560 | MG132

Chollangi, S. et al. (2012) "The hemerythrin-like domain within F-box and leucine-rich repeat protein 5 communicates cellular iron and oxygen availability by distinct mechanisms." The Journal of biological chemistry. 2012 May 30;

22648410 | MG132

Zhang, Y. et al. (2012) "Regulation of cell cycle progression by forkhead transcription factor FOXO3 through its binding partner DNA replication factor Cdt1." Proc. Natl. Acad. Sci. U.S.A. 109(15): 5717–5722.

22451935 | MG132

Inhibitor

23475958 | ubiquitin aldehyde
23318177 | 19S inhibitor b-AP15
epoximycin | 23437325
23535459 | MG132
23486064 | ubiquitin aldehyde
23424652 | HA-Ub vinyl sulfone
23458873 | MG-132 proteosome inhibitor
23541081 | MG-132
23541081 | ubiquitin aldehyde
23449802 | ubiquitin aldehyde
23512198 | ubiquitin aldehyde

Zhang, S. et al. (2012) "GST:Identification of RNF8 as a ubiquitin ligase involved in targeting the p12 subunit of DNA polymerase δ for degradation in response to DNA damage." J. Biol. Chem.

23233665 | ATP regenerating solution (ENS), ubiquitin aldehyde, HeLa S100 fraction, Recombinant human E1 ubiquitin activating enzyme, Recombinant human ubiquitin, Recombinant human ubiquitin K48R, Recombinant human ubiquitin K63R, Recombinant human UbcH5c, Recombinant human Cdc34, Recombinant human UbcH2

Frisan, T. et al. (2012) "Ubiquitin C-terminal hydrolase-L1 interacts with adhesion complexes and promotes cell migration, survival, and anchorage independent growth." FASEB J.

22932395 | Ub-VS functional probe

Henle, A.M. et al. (2012) "Enzymatic Discovery of a HER-2/neu Epitope That Generates Cross-Reactive T Cells." J. Immunol.

23180824 | Suc-LLVY-AMC, synthetic lactacystin, reaction buffer (25 mM HEPES, 0.5 mM EDTA, pH 7.6), immunoproteasome, PA28 activator alpha subunit, purified human 20S proteasome

Guan, B. et al. (2012) "Functional Analysis of In-frame Indel ARID1A Mutations Reveals New Regulatory Mechanisms of Its Tumor Suppressor Functions." Neoplasia 14(10): 986–993.

23097632 | MG132

Cosper, P.F. et al. (2012) "Interferon-γ Causes Cardiac Myocyte Atrophy via Selective Degradation of Myosin Heavy Chain in a Model of Chronic Myocarditis." Am. J. Pathol. 181(6): 2038–2046.

23058369 | MG-132

Carvalho, A.N. et al. (2012) "Ubiquitin-Proteasome System Impairment and MPTP-Induced Oxidative Stress in the Brain of C57BL/6 Wild-type and GSTP Knockout Mice." Mol. Neurobiol.

23129554 | Z-leucine-leucine-glutamic acid-7-amido-4-methylcoumarin (Z-LLE-AMC), Boc-leucine-arginine-arginine-7-amido-4-methylcoumarin (Boc-LRR-AMC), succcinyl-leucine-leucine-valine-tyro-sine-7-amido-4-methylcoumarin (Suc-LLVY-AMC), isopeptidase inhibitor (Ubiquitin aldehyde [Ubal]), MG132,

Caldeira, M.V. et al. (2012) "Excitotoxic stimulation downregulates the ubiquitin-proteasome system through activation of NMDA receptors in cultured hippocampal neurons." Biochim. Biophys. Acta.

23069389 | HA-Ub-VS

Blount, J.R. et al. (2012) "Ubiquitin-Specific Protease 25 Functions in Endoplasmic Reticulum-Associated Degradation." PLoS ONE 7(5): e36542.

22590560 | MG132

Chollangi, S. et al. (2012) "The hemerythrin-like domain within F-box and leucine-rich repeat protein 5 communicates cellular iron and oxygen availability by distinct mechanisms." The Journal of biological chemistry. 2012 May 30;

22648410 | MG132

Zhang, Y. et al. (2012) "Regulation of cell cycle progression by forkhead transcription factor FOXO3 through its binding partner DNA replication factor Cdt1." Proc. Natl. Acad. Sci. U.S.A. 109(15): 5717–5722.

22451935 | MG132

Kensche, T. et al. (2012) "Analysis of NF-κB essential modulator (NEMO) binding to linear and lysine-linked ubiquitin chains and its role in the activation of NF-κB." The Journal of biological chemistry. 2012 May 17;

22605335 | ubiquitin aldehyde

Long, M.J.C. et al. (2012) "Inhibitor Mediated Protein Degradation." Chemistry & Biology 19(5): 629–637.

22633414 | IU1

Tsuji, T. et al. (2012) "Heat Shock Protein 90-Mediated Peptide-Selective Presentation of Cytosolic Tumor Antigen for Direct Recognition of Tumors by CD4+ T Cells." J. Immunol. 188(8): 3851–3858.

22427632 | epoxomicin

Long, M.J.C. et al. (2012) "Inhibitor Mediated Protein Degradation." Chemistry & Biology 19(5): 629–637.

22633414 | lactacystin

Ubiquitin

Chicooree, N. et al. (2013) "A novel approach to the analysis of SUMOylation with the independent use of trypsin and elastase digestion followed by database searching utilising consecutive residue addition to lysine." Rapid Commun. Mass Spectrom. 27(1): 127–134.

23239325 | di-Ub-K48, di-Ub-K63, di-SUMO-2, di-SUMO-3

Hebron, M.L. et al. (2012) "Parkin ubiquitinates Tar-DNA binding protein-43 (TDP-43) and promotes its cytosolic accumulation via interaction with histone deacetylase 6 (HDAC6)." J. Biol. Chem.

23258539 | recombinant human ubiquitin, recombinant UbcH7, E1 recombinant enzyme,
23453969 | ubiquitin
23469023 | His6-tagged polyubiquitin chain (K48-linked)
23469023 | His6-tagged polyubiquitin chain (K63-linked)
23455478 | ubiquitin
23359680 | ubiquitin
23459942 | Ub-K0
23459942 | Ub-K0
23459942 | Ub-K63
23459942 | Ub-WT
23361369 | ubiquitinated protein substrate di-Ub-K48
23277564 | lysineless ubiquitin
23146630 | di-ubiquitins
23345618 | FLAG-ubiquitin
23287719 | Ub
23379478 | ubiquitin
23332158 | “Ubiquitination assays were performed using enzymes obtained from Boston Biochem”
23362280 | ubiquitin
23555887 | HA-ubiquitin
23486064 | ubiquitin wild type
23486064 | ubiquitin-k0
23269275 | FLAG-ubiquitin
23498734 | HA::ubiquitin
23150437 | Me-Ub

SUMO

Chicooree, N. et al. (2013) "A novel approach to the analysis of SUMOylation with the independent use of trypsin and elastase digestion followed by database searching utilising consecutive residue addition to lysine." Rapid Commun. Mass Spectrom. 27(1): 127–134.

23239325 | di-Ub-K48, di-Ub-K63, di-SUMO-2, di-SUMO-3

Cotto-Rios, X.M. et al. (2012) "Deubiquitinases as a signaling target of oxidative stress." Cell Rep 2(6): 1475–1484.

23219552 | UbVME, recombinant ubiquitin chain K48-linked, UCH-L1, SENP1-CD, STAM, recombinant ubiquitin chain K63-linked, SUMO1-vs, USP7, AMSH,

Guo, Z. et al. (2012) "Sequential Posttranslational Modifications Program FEN1 Degradation during Cell-Cycle Progression." Molecular cell.

22749529 | ubiquitination reaction buffer, UBE1, UBE2M, HeLa S100 fraction, SUMOylation kits, ubiquitination kit

Hu, X. et al. (2012) "Rap80 Protein Recruitment to DNA Double-strand Breaks Requires Binding to Both Small Ubiquitin-like Modifier (SUMO) and Ubiquitin Conjugates." J. Biol. Chem. 287(30): 25510–25519.

22689573 | SUMO2 3– 8, agarose-SUMO2 beads, Ub Lys-63 2–7,

Liu, Y. et al. (2012) "NF-κB Repression by PIAS3 Mediated RelA SUMOylation." PLoS ONE 7(5): e37636.

22649547 | SAE1/SAE2

Luo, K. et al. (2012) "Sumoylation of MDC1 is important for proper DNA damage response." The EMBO journal.  2012 May 25;

22635276 | SUMO-activating enzyme (UBA2), SUMO1, SUMO2, SUMO3

Rendtlew Danielsen, J. et al. (2012) "DNA damage-inducible SUMOylation of HERC2 promotes RNF8 binding via a novel SUMO-binding Zinc finger." J. Cell Biol. 197(2): 179–187.

22508508 | SUMO1, SUMO2

Osula, O. et al. (2012) "Identification of protein SUMOylation sites by mass spectrometry using combined microwave-assisted aspartic acid cleavage and tryptic digestion." J Mass Spectrom 47(5): 644–654.

22576878 | di-SUMO-2, di-SUMO-3

Chang, C., et al (2011) “A Novel Cyclic AMP/Epac1/CaMKI Signaling Cascade Promotes GCM1 Desumoylation and Placental Cell Fusion.” Mol. Cell. Biol. 31: 3820 - 3831.

21791615 | His-SUMO1

Chang, C., et al (2011) “A Novel Cyclic AMP/Epac1/CaMKI Signaling Cascade Promotes GCM1 Desumoylation and Placental Cell Fusion.” Mol. Cell. Biol. 31: 3820 - 3831.

21791615 | recombinant SAE1/SAE2

Galisson, F., et al. (2011) “A Novel Proteomics Approach to Identify SUMOylated Proteins and Their Modification Sites in Human Cells.” Molecular and Cellular Proteomics, 10: M110.004796.

21098080 | SASE1/SAE2 heterodimer

Wyatt, D., et al. (2011) “Small Ubiquitin-like Modifier Modification of Arrestin-3 Regulates Receptor Trafficking.” The Journal of Biological Chemistry, 286: 3884-3893.

21118812 | anti-SUMO-1 polyclonal antibody

Koike, A, et al. (2010) “Recruitment of Phosphorylated NPM1 to Sites of DNA Damage through RNF8-Dependent Ubiquitin Conjugates.” Cancer Res. 70: 6746 - 6756.

20713529 | SUMO polymers

Chu, Y. and Yang, X. (2010) "SUMO E3 ligase activity of TRIM proteins.” Oncogene 30(9): 1108–1116.

20972456 | SUMO E1, SUMO1, SUMO2

Shanks. J., et al. (2009) "Burkholderia mallei tssM Encodes a Putative Deubiquitinase That Is Secreted and Expressed inside Infected RAW 264.7 Murine Macrophages." Infect. Immun. 77 (4): 1636-1648.

19168747 | Di-SUMO-2, Di-SUMO-3

Zunino, R., et al.(2009) "Translocation of SenP5 from the Nucleoli to the Mitochondria Modulates DRP1-dependent Fission during Mitosis." J. Biol. Chem. 284: 17783-17795.

19411255 | His6-SUMO1, SUMO activating enzyme

MacPherson, M.J., et al. (2009) "The CTCF Insulator Protein Is Post-translationally Modified by SUMO." Mol. Cell. Biol. 29(3): 714-725.

19029252 | SUMO1, SUMO2, SUMO3

Dawlaly, M.M., et al. (2008) "Resolution of Sister Centromeres requires RanBP2-mediated SUMOylation of Topoisomerase II[alpha]." Cell 133: 103-115.

18394993 | SUMO1 conjugation kit

Lima, C.D. and Reverter, D. (2008) "Structure of the Human SENP7 Catalytic Domain and Poly-SUMO Deconjugation Activities for SENP6 and SENP7." J. Biol. Chem. 283 (46): 32045-32055.

18799455 | di-SUMO2, di-SUMO3, poly-SUMO2, poly-SUMO3

Benson, M. D., et al. (2007). "SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5." PNAS 104(6): 1805-1810.

17261810 | lactacystin

Chou, C.-C., et al. (2007). "SUMO modification regulates the DNA-binding activity of GCMa." J. Biol. Chem.: 282(37): 27239-27249.

17646165 | recombinant SAE1/SAE2

Catic A., et al. (2007) "Screen for ISG15-cross-reactive Deubiquitinases." PLoS ONE 2(7): E769.

17653289 | GST-SUMO1

Miller, S. L. H., et al. (2007). "Ubiquitin-interacting Motifs Inhibit Aggregation of PolyQ-expanded Huntingtin." J. Biol. Chem. 282(13): 10096-10103.

17276991 | SUMO-1

Rytkonen, A., et al. (2007) "SseL, a Salmonella deubiquitinase required for macrophage killing and virulence4." PNAS 104(9): 3502-3507.

SUMO-1-AMC

Avvakumov, G. V., et al. (2006). "Amino-terminal dimerization, NRDP1 (FLRF) - rhodanese interaction, and inhibited catalytic domain conformation of the ubiquitin specific protease 8 (USP8/UBPY)." J. Biol. Chem.281(49):38061-38070.

17035239 | SUMO-AMC

NEDD8

23300442 | NEDD8

Zhong, H.-J. et al. (2012) "A Metal-Based Inhibitor of NEDD8-Activating Enzyme." PLoS ONE 7(11): e49574.

23185368 | anti-Ubc12 rabbit polyclonal antibody, NEDD8 Conjugation Initiation Kit

Na, C.-H. et al. (2012) "Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis." J. Proteome Res. 11(9):4722-32.

22871113 | K48 di-Ub, recombinant purified ISG15, recombinant purified Nedd8, recombinant purified Ub

Gastaldello, S. et al. (2012) "Herpes virus deneddylases interrupt the cullin-RING ligase neddylation cycle by inhibiting the binding of CAND1." Journal of molecular cell biology. 2012 Apr 3;

22474075 | Nedd8-VS

Capodagli, G., et al. (2011) “Structural analysis of a viral ovarian tumor domain protease from the Crimean-Congo Hemorrhagic Fever virus in complex with covalently bonded ubiquitin.” Journal of Virology, 85: 3621-3630.

21228232 | NEDD8-AMC

Wu, K., et al. (2011) “Mono-ubiquitination Drives Nuclear Export of the Human DCN1-like Protein hDCNL1.” J. Biol. Chem. 286: 34060 - 34070.

21813641 | Nedd8, PK-Nedd8

Hosp, J., et al. (2011) "A tobacco homolog of DCN1 is involved in cellular reprogramming and in developmental transitions.” doi:10101/npre.2011.5728.1

10101 | NEDD8- agarose

Artavanis-Tsakonas, K., et al. (2010) “Characterization and Structural Studies of the Plasmodium falciparum Ubiquitin and Nedd8 Hydrolase UCHL3.” J. Biol. Chem. 285: 6857 - 6866.

20042598 | NedP1

Choi, Y. et al. (2010) "The Human Cdc34 Carboxyl Terminus Contains a Non-covalent Ubiquitin Binding Activity That Contributes to SCF-dependent Ubiquitination." J. Biol. Chem. 285: 17754 - 17762.

20353940 | E1 enzyme for Nedd8 (APP-BP1/Uba3)

Gastaldello, S., et al. (2010) "A deneddylase encoded by Epstein–Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases.” Nature cell biology 12(4): 351–361.

20190741 | NEDD8-VS functional probe

Cooper, E.M., et al. (2009) "K63-specific deubuiquitination by two JAMM/MPN+ complexes:BRISC-associated Brcc-36 and proteasomal Poh1." EMBO J. 28(6) 621-631 .

Nedd8

Kim, A.Y., et al. (2008) “SCCRO (DCUN1D1) Is an Essential Component of the E3 Complex for Neddylation.” J. Biol. Chem. 283(48): 33211-33220.

18826954 | NEDD8

Heuzé, M.L., et al. (2008) "ASB2 targets filamins A and B to proteasomal degradation." Blood 112(13): 5130-5140.

18799729 | NEDD8

Miller, S. L. H., et al. (2007). "Ubiquitin-interacting Motifs Inhibit Aggregation of PolyQ-expanded Huntingtin." J. Biol. Chem. 282(13): 10096-10103.

17276991 | His-tagged NEDD8

Avvakumov, G. V., et al. (2006). "Amino-terminal dimerization, NRDP1 (FLRF) - rhodanese interaction, and inhibited catalytic domain conformation of the ubiquitin specific protease 8 (USP8/UBPY)." J. Biol. Chem.281(49):38061-38070.

17035239 | NEDD8-AMC

Oved, S., Y. Mosesson, et al. (2006). "Conjugation to Nedd8 Instigates Ubiquitylation and Down-regulation of Activated Receptor Tyrosine Kinases." J. Biol. Chem. 281(31): 21640-21651.

16735510 | anti-Nedd8, polyclonal

ISG15

Na, C.-H. et al. (2012) "Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis." J. Proteome Res. 11(9):4722-32.

22871113 | K48 di-Ub, recombinant purified ISG15, recombinant purified Nedd8, recombinant purified Ub

Basters, A. et al. (2012) "High yield expression of catalytically active USP18 using a Trigger Factor fusion system." BMC Biotechnol. 12(1): 56.

22916876 | HA-ISG15 vinyl sulfone (ISG15-VS), ISG15-amidomethyl coumarin (AMC), HA-Ubiquitin (Ub-VS)

Capodagli, G., et al. (2011) “Structural analysis of a viral ovarian tumor domain protease from the Crimean-Congo Hemorrhagic Fever virus in complex with covalently bonded ubiquitin.” Journal of Virology, 85: 3621-3630.

21228232 | ISG15-AMC

James, T., et al. (2011) “Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease.” PNAS, 108: 2222-2227.

21245344 | ISG15–AMC

Malakhova O.A. and Zhang D-E. (2008) "ISG15 Inhibits Nedd4 Ubiquitin E3 Activity and Enhances the Innate Antiviral Response." J. Biol. Chem. 283: 8783-8787.

18287095 | ISG15

Ratia, K, et al. (2008). "A non-covalent class of papain-like protease/deubiquitinase inhibitors blocks SARS virus replication." PNAS 105 (42): 16119-16124 .

18852458 | ISG15-AMC

Catic A., et al. (2007) "Screen for ISG15-cross-reactive Deubiquitinases." PLoS ONE 2(7): E769.

17653289 | ISG15

UFM1

Bacik, J., et al. (2010) “Crystal Structure of the Human Ubiquitin-activating Enzyme 5 (UBA5) Bound to ATP: MECHANISTIC INSIGHTS INTO A MINIMALISTIC E1 ENZYME.” J. Biol. Chem. 285: 20273 - 20280.

20368332 | UFM1

Autophagy

Havens, C. G., et al. (2006) "Regulation of Late G1/S Phase Transition and APCCdh1 by Reactive Oxygen Species." Mol. Cell. Biol. 26(12): 4701-4711.

16738333

FAT10

23452859 | recombinant FAT10

Antibodies

23394999 | rabbit anti-UBE2M antibody
23344957 | anti-UbcH5
23375434 | ubiquitin antibody

Pulvino, M. et al. (2012) "Inhibition of proliferation and survival of diffuse large B-cell lymphoma cells by a small-molecule inhibitor of the ubiquitin-conjugating enzyme Ubc13-Uev1A." Blood.

22791293 | anti-UbcH5

Hu, R. et al. (2012) "Distinct transcriptional programs mediated by the ligand-dependent full-length androgen receptor and its splice variants in castration-resistant prostate cancer." Cancer research.

22710436 | anti-UBE2C

Maezawa, S. et al. (2012) "Ubiquitylation of terminal deoxynucleotidyltransferase inhibits its activity." PLoS ONE 7(7): e39511.

22808041 | rabbit pAb against UbcH5, rabbit pAb against UbcH6, rabbit pAb against UbcH7

Delfino, G.B. et al. (2012) "Quadriceps Muscle Atrophy After Anterior Cruciate Ligament Transection Involves Increased mRNA Levels of Atrogin-1, Muscle Ring Finger 1, and Myostatin." Am J Phys Med Rehabil.

22854904 | Ubiquitin Polyclonal Antibody

Ali, A.M. et al. (2012) "FAAP20: a novel ubiquitin-binding FA nuclear core-complex protein required for functional integrity of the FA-BRCA DNA repair pathway." Blood 119(14): 3285–3294.

22343915 | ubiquitin antibody

Zhang, Y.-G. et al. (2012) "Axin1 prevents salmonella invasiveness and inflammatory response in intestinal epithelial cells." PLoS ONE 7(4): e34942.

22509369 | anti-sumo1

Diniz, G.P. et al. (2012) "New Insight into the mechanisms associated to rapid effect of T3 on AT1R expression." Journal of molecular endocrinology. 2012 Apr 23;

22525353 | polyclonal antibody against ubiquitin

Kudryashova, E. et al. (2012) "Satellite cell senescence underlies myopathy in a mouse model of limb-girdle muscular dystrophy 2H." J. Clin. Invest. 122(5): 1764–1776.

22505452 | rabbit anti–SUMO-1

Song, M., et al. (2011) “Nuclear PTEN Regulates the APC-CDH1 Tumor-Suppressive Complex in a Phosphatase-Independent Manner.” Cell, 144: 187-199.

21241890 | Anti-UbcH10, anti-UbcH5, Anti-UbcH10 (A-650), (A-615)

Wang, H., et al. (2011) “CCI-779 Inhibits Cell-Cycle G2–M Progression and Invasion of Castration-Resistant Prostate Cancer via Attenuation of UBE2C Transcription and mRNA Stability.” Cancer Res. 71: 4866 - 4876.

21593191 | anti-UBE2C

Chen, A., et al. (2011) “Enzyme E2 from Chinese White Shrimp Inhibits Replication of White Spot Syndrome Virus and Ubiquitinates Its RING Domain Proteins.” J. Virol. 85: 8069 - 8079.

21680526 | anti-ubiquitin / A-100

Chen, L., et al. (2011) “Sts1 Plays a Key Role in Targeting Proteasomes to the Nucleus.” The Journal of Biological Chemistry, 286: 3104-3118.

21075847 | Anti-His6 monoclonal antibody

Rakovic, A., et al. (2011) “Mutations in PINK1 and Parkin Impair Ubiquitination of Mitofusins in Human Fibroblasts.” PLoS one, 6: e16746. doi:10.1371/journal.pone.0016746.

21408142 | anti-ubiquitin

Wyatt, D., et al. (2011) “Small Ubiquitin-like Modifier Modification of Arrestin-3 Regulates Receptor Trafficking.” The Journal of Biological Chemistry, 286: 3884-3893.

21118812 | anti-Ubc9 polyclonal antibody

Kaiser, S.E., et al. (2011) "Protein standard absolute quantification (PSAQ) "method for the measurement of cellular ubiquitin pools.” Nature methods 8(8): 691–696.

Ubiquitin antibody (A-100)

Kaiser, S.E., et al. (2011) "Protein standard absolute quantification (PSAQ) method for the measurement of cellular ubiquitin pools.” Nature methods 8(8): 691–696.

21743460 | Ubiquitin antibody (A-100)

Mansfeld, J., et al. (2011) "APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.” Nature cell biology 13(10): 1234–1243.

21926987 | anti-UbcH10

Bacik, J., et al. (2010) “Crystal Structure of the Human Ubiquitin-activating Enzyme 5 (UBA5) Bound to ATP: MECHANISTIC INSIGHTS INTO A MINIMALISTIC E1 ENZYME.” J. Biol. Chem. 285: 20273 - 20280.

20368332 | anti-UFM1 primary rabbit polyclonal antibody

Kavazis, A. N., et al. (2010) “Short-term exercise training protects against doxorubicin-induced cardiac mitochondrial damage independent of HSP72.” Am. J. Physiol. Heart Circ. Physiol. 299: H1515-H1524.

20833957 | ubiquitin-protein conjugates

Tang, W., et al. (2010) “Emi2-mediated Inhibition of E2-substrate Ubiquitin Transfer by the Anaphase-promoting Complex/Cyclosome through a D-Box–independent Mechanism.” Mol. Biol. Cell 21: 2589 - 2597.

20534816 | anti UbcH10

Kumar, B., et al. (2010) “The C Terminus of Rpt3, an ATPase Subunit of PA700 (19 S) Regulatory Complex, Is Essential for 26 S Proteasome Assembly but Not for Activation.” J. Biol. Chem. 285: 39523-39535.

20937828 | anti-Rpt3

Liang, J., et al. (2010) “MCP-induced protein 1 deubiquitinates TRAF proteins and negatively regulates JNK and NF-B signaling.” J. Exp. Med. 207: 2959-2973.

21115689 | anti-ubiquitin

Kits

23442071 | Ubiquitin-Protein Conjugation kit

Guo, Z. et al. (2012) "Sequential Posttranslational Modifications Program FEN1 Degradation during Cell-Cycle Progression." Molecular cell.

22749529 | ubiquitination reaction buffer, UBE1, UBE2M, HeLa S100 fraction, SUMOylation kits, ubiquitination kit

Lim, J.H. et al. (2012) "CYLD negatively regulates transforming growth factor-β-signalling via deubiquitinating Akt." Nat Commun 3: 771

22491319 | In vitro ubiquitination and deubiquitination assay kit

Osula, O. et al. (2012) "Identification of protein SUMOylation sites by mass spectrometry using combined microwave-assisted aspartic acid cleavage and tryptic digestion." J Mass Spectrom 47(5): 644–654.

22576878 | SUMOylation kit containing SUMO-1

Zhou, X.H. et al. (2012) "A three generation study with high-lysine transgenic rice in Sprague-Dawley rats." Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 50(6): 1902–1910.

22510491 | rat sex hormone ELISA kit

Hodson, C., et al. (2011) “Structural Analysis of Human FANCL, the E3 Ligase in the Fanconi Anemia Pathway.” J. Biol. Chem. 286: 32628 - 32637.

21775430 | His-UbcH7

Sung, C.K., et al. (2011) “Transcriptional and post-translational regulation of the quiescence factor and putative tumor suppressor p150Sal2.” The FASEB Journal fj.10-173674; published ahead of print January 12, 2011,doi:10.1096/fj.10-173674.

21228219 | ubiquitin conjugation reaction buffer kit (SK-10)

Dolan, P.J. and Johnson, G. (2010) “A Caspase Cleaved Form of Tau Is Preferentially Degraded through the Autophagy Pathway.” J. Biol. Chem. 285: 21978 - 21987.

20466727 | UbcH5b

Cohen, S., et al. (2009) "During muscle atrophy, thick, but not thin, filament components are degraded by MuRF1-dependent ubiquitylation." J. Cell Biol. 185: 1083-1095.

19506036 | UbcH13, Uev1 E2-conjugating enzyme

Pacheco, C.D., et al. (2009) "Tau deletion exacerbates the phenotype of Niemann–Pick type C mice and implicates autophagy in pathogenesis." Hum. Mol. Genet. 18(5): 956-965.

19074461 | 20S Proteasome Assay Kit, SDS-Activation Format

Sun, L., et al. (2009) “JFK, a Kelch domain-containing F-box protein, links the SCF complex to p53 regulation.” PNAS 106: 10195-10200.

ubiquitin-protein conjugation kit

Stanisic, V., et al (2009) "OTU Domain-containing Ubiquitin Aldehyde-binding Protein 1 (OTUB1) Deubiquitinates Estrogen Receptor (ER)a and Affects ERa Transcriptional Activity." J. Biol. Chem. 284: 16135-16145.

19383985 | ubiquitination kit, K-960

Marfella, R., et al. (2008) "Proteasome Activity as a Target of Hormone Replacement Therapy-Dependent Plaque Stabilization in Postmenopausal Women." Hypertension 51: 1135-41.

proteasome 20S SDS activation kit

Marfella, R., et al. (2008) "Effects of Ubiquitin-Proteasome System Deregulation on the Vascular Senescence and Atherosclerosis Process in Elderly Patients." J. Gerontol. A Biol. Sci. Med. Sci. 63: 200-3.

sodium dodecyl sulfate [SDS]-activation kit

Marfella, R., et al. (2007). "Morning Blood Pressure Surge as a Destabilizing Factor of Atherosclerotic Plaque: Role of Ubiquitin-Proteasome Activity." Hypertension 49(4): 784-791.

proteasome-20S SDS-activation kit

Esposito, K., et al. (2006). "Rosiglitazone Cools Down Inflammation in the Metabolic Syndrome." Arterioscler. Thromb. Vasc. Biol. 26(6): 1413-1414.

16709957 | SDS-activation kit

Samuel, T., et al. (2006). "Distinct BIR Domains of cIAP1 Mediate Binding to and Ubiquitination of Tumor Necrosis Factor Receptor-associated Factor 2 and Second Mitochondrial Activator of Caspases." J. Biol. Chem. 281(2): 1080-1090.

16282325 | ubiquitin-protein conjugation kit

Marfella, R., et al. (2006). "The Ubiquitin-Proteasome System and Inflammatory Activity in Diabetic Atherosclerotic Plaques: Effects of Rosiglitazone Treatment." Diabetes 55(3): 622-632.

16505224 | proteasome 20S SDS activation kit

Gurova K. V., et al. (2005) "Small molecules that reactivate p53 in renal cell carcinoma reveal a NF-κB-dependent mechanism of p53 suppression in tumors." PNAS 102(48): 17448-17453.

16287968 | kit for assaying proteasomal activity

Buffers, Solutions, Standards

23455478 | Mg-ATPb
23345618 | 10x energy regeneration solution
23362280 | ubiquitination reaction buffer
23555887 | ATP-Mg2+
23269275 | ATP
23150437 | Mg2+-ATP
23314748 | ATP
23469016 | 20X energy regeneration mix
23339190 | energy regeneration system
23541081 | 10x ubiquitination buffer
23212909 | energy regeneration solution

Zhang, S. et al. (2012) "GST:Identification of RNF8 as a ubiquitin ligase involved in targeting the p12 subunit of DNA polymerase δ for degradation in response to DNA damage." J. Biol. Chem.

23233665 | ATP regenerating solution (ENS), ubiquitin aldehyde, HeLa S100 fraction, Recombinant human E1 ubiquitin activating enzyme, Recombinant human ubiquitin, Recombinant human ubiquitin K48R, Recombinant human ubiquitin K63R, Recombinant human UbcH5c, Recombinant human Cdc34, Recombinant human UbcH2

Guo, Z. et al. (2012) "Sequential Posttranslational Modifications Program FEN1 Degradation during Cell-Cycle Progression." Molecular cell.

22749529 | ubiquitination reaction buffer, UBE1, UBE2M, HeLa S100 fraction, SUMOylation kits, ubiquitination kit

Zhao, Y. et al. (2012) "Non-Covalent Interaction between Polyubiquitin and GTP Cyclohydrolase 1 Dictates Its Degradation." PLoS ONE 7(9): e43306.

22984419 | mixed ubiquitin chains (Ub2~7), sample buffer, Tetraubiquitin (Ub4), UIM-agarose

Zang, Y. et al. (2012) "Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage." Rice 5(1): 24.

10.1186/1939-8433-5-24 | E1 enzyme, K48R ubiquitin, K63R ubiquitin, reaction buffer, Ub, Ubc13, Uev1A, ubiquitin-AMC, nonbiotinylated USP2 catalytic domain, USP10, USP47, USP5, USP7

Merkx, R. et al. (2012) "Synthesis and Evaluation of a Selective Fluorogenic Pup Derived Assay Reagent for Dop, a Potential Drug Target in Mycobacterium tuberculosis." Chembiochem 13(14): 2056–2060.

22927162 | energy regeneration solution

Humphrey, R.K. et al. (2012) "Lysine 63-linked Ubiquitination Modulates Mixed Lineage Kinase-3 Interaction with JIP1 Scaffold protein in Cytokine-induced Pancreatic Beta Cell Death." J. Biol. Chem.

23172226 | Mg-ATP mix cofactor mix, Myc-ubiquitin, recombinant UbE1,UbeH13-Uev1a heterodimer complex, Ub conjugation buffer,

Henle, A.M. et al. (2012) "Enzymatic Discovery of a HER-2/neu Epitope That Generates Cross-Reactive T Cells." J. Immunol.

23180824 | Suc-LLVY-AMC, synthetic lactacystin, reaction buffer (25 mM HEPES, 0.5 mM EDTA, pH 7.6), immunoproteasome, PA28 activator alpha subunit, purified human 20S proteasome

Marfella, R. et al. (2012) "Dipeptidyl Peptidase 4 Inhibition May Facilitate Healing of Chronic Foot Ulcers in Patients with Type 2 Diabetes." Experimental Diabetes Research 2012: 1–11.

23197976 | SDS-activation kit

Huett, A. et al. (2012) "The LRR and RING Domain Protein LRSAM1 Is an E3 Ligase Crucial for Ubiquitin-Dependent Autophagy of Intracellular Salmonella Typhimurium." Cell Host Microbe 12(6): 778–790.

23245322 | ubiquitination buffer, HA-tagged free ubiquitin, His-tagged free ubiquitin

Kensche, T. et al. (2012) "Analysis of Nuclear Factor-κB (NF-κB) Essential Modulator (NEMO) Binding to Linear and Lysine-linked Ubiquitin Chains and Its Role in the Activation of NF-κB." J. Biol. Chem. 287(28): 23626–23634.

22605335 | creatine phosphokinase, HeLa-S-100 fraction II, ubiquitin, ubiquitin aldehyde

Sambuughin, N. et al. (2012) "KBTBD13 interacts with Cullin 3 to form a functional ubiquitin ligase." Biochemical and biophysical research communications. 2012 Apr 20;

22542517 | ubiquitin reaction buffer

Luo, K. et al. (2012) "Sumoylation of MDC1 is important for proper DNA damage response." The EMBO journal.  2012 May 25;

22635276 | E1 stop buffer

Bell, J.L. et al. (2012) "TRIM16 Acts as an E3 Ubiquitin Ligase and Can Heterodimerize with Other TRIM Family Members." PLoS ONE 7(5): e37470.

22629402 | In Vitro Ubiquitination Buffer containing E1 and E2

Luo, K. et al. (2012) "Sumoylation of MDC1 is important for proper DNA damage response." The EMBO journal.  2012 May 25;

22635276 | 10 x reaction buffer

Substrate Proteins

23287719 | Ub-7-amido-4-methylcoumarin (Ub-AMC)
23318177 | Suc-LLVY-AMC
23318177 | Suc-LLVY-AMC
23437325 | Ac-Arg-Leu-Arg-AMC
23437325 | Ac-Nle-Pro-Nle-Asp-AMC
23437325 | Suc-Leu-Leu-Val-Tyr-AMC
23459205 | His-s5a
23463011 | Ub-AFC
23375434 | ubiquitin-AMC
23401522 | Ub-AMC

Guglielmotto, M. et al. (2012) "Aβ 1-42-mediated down-regulation of Uch-L1 is dependent on NF-κB activation and impaired BACE1 lysosomal degradation." Aging cell.

22726800 | ubiquitin-7-amino-4-methylcoumarin (ubiquitin-AMC)

Chung, D.-W.D. et al. (2012) "Characterization of the Ubiquitylating Components of the Human Malaria Parasite’s Protein Degradation Pathway." PLoS ONE 7(8): e43477.

22912882 | re-energizing buffer, human recombinant UBE1 E1, Suc-LLVY-AMC, human recombinant UBC E2, biotin conjugated ubiquitin, ubiquitin,

Kanga, S. et al. (2012) "A deubiquitylating complex required for neosynthesis of a yeast mitochondrial ATP synthase subunit." PLoS ONE 7(6): e38071.

22723847 | Ubiquitin-AMC

Hochrainer, K. et al. (2012) "Reperfusion rather than ischemia drives the formation of ubiquitin aggregates after middle cerebral artery occlusion." Stroke 43(8): 2229–2235.

22700531 | synthetic peptide Succinyl-Leu-Leu-Val-Tyr-7- Amino-4-methylcoumarin

Zhu, Y. et al. (2012) "Synthesis and inhibitory activities against colon cancer cell growth and proteasome of alkylresorcinols." J. Agric. Food Chem. 60(35): 8624–8631.

22897570 | human 20S proteasome, proteasome activator PA28, Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY-AMC)

Neilsen, P.M. et al. (2012) "New 26S Proteasome Inhibitors with High Selectivity for Chymotrypsin-Like Activity and p53-Dependent Cytotoxicity." ACS Chem. Biol.

23190346 | purified rabbit20S proteasome, Suc-LLVY-AMC

Blackburn C, Barrett C, Blank JL, et al. "Optimization of a series of dipeptides with a P3 β-neopentyl asparagine residue as non-covalent inhibitors of the chymotrypsin-like activity of human 20S proteasome." Med. Chem. Commun. 2012 May 30;3(6):710–719. 

10.1039/C2MD20060K | purified human erythrocyte and peripheral blood monocyte 20S proteasomes, recombinant proteasomal activator PA28alpha, Z-LLE-AMC

Henle, A.M. et al. (2012) "Enzymatic Discovery of a HER-2/neu Epitope That Generates Cross-Reactive T Cells." J. Immunol.

23180824 | Suc-LLVY-AMC, synthetic lactacystin, reaction buffer (25 mM HEPES, 0.5 mM EDTA, pH 7.6), immunoproteasome, PA28 activator alpha subunit, purified human 20S proteasome

Carvalho, A.N. et al. (2012) "Ubiquitin-Proteasome System Impairment and MPTP-Induced Oxidative Stress in the Brain of C57BL/6 Wild-type and GSTP Knockout Mice." Mol. Neurobiol.

23129554 | Z-leucine-leucine-glutamic acid-7-amido-4-methylcoumarin (Z-LLE-AMC), Boc-leucine-arginine-arginine-7-amido-4-methylcoumarin (Boc-LRR-AMC), succcinyl-leucine-leucine-valine-tyro-sine-7-amido-4-methylcoumarin (Suc-LLVY-AMC), isopeptidase inhibitor (Ubiquitin aldehyde [Ubal]), MG132,

Lansdell, T.A. et al. (2012) "Non-competitive modulation of the proteasome by imidazoline scaffolds overcome bortezomib resistant and delay MM tumor growth in vivo." ACS Chem. Biol.

23198928 | purified proteasome particles, Suc-LLVY-AMC, Z-ARR-AMC, Z-LLE-AMC

Rodriguez, K.A. et al. (2012) "Altered composition of liver proteasome assemblies contributes to enhanced proteasome activity in the exceptionally long-lived naked mole-rat." PLoS ONE 7(5): e35890.

22567116 | butoxycarbonyl-LeuArgArg-AMC (Boc-LRR-AMC)

Rodriguez, K.A. et al. (2012) "Altered composition of liver proteasome assemblies contributes to enhanced proteasome activity in the exceptionally long-lived naked mole-rat." PLoS ONE 7(5): e35890.

22567116 | carbobenzoxy-LeuLeuGlu-AMC (Z-LLE-AMC)

Rodriguez, K.A. et al. (2012) "Altered composition of liver proteasome assemblies contributes to enhanced proteasome activity in the exceptionally long-lived naked mole-rat." PLoS ONE 7(5): e35890.

22567116 | succinyl-LeuLeuValTyr-7-amido-4-methylc​oumarin(Suc-LLVY-AMC)

Chou, Y.-W. et al. (2012) "Differential domain structure stability of the severe acute respiratory syndrome coronavirus papain-like protease." Arch. Biochem. Biophys. 520(2): 74–80.

22391227 | ubiquitin-AFC

Reverdy, C. et al. (2012) "Discovery of Specific Inhibitors of Human USP7/HAUSP Deubiquitinating Enzyme." Chem. Biol. 19(4): 467–477.

22520753 | ubiquitin-AMC (ubiquitin-7-amido-4-methylcoumarin), SENP1, U-550

Affinity Matrices

23459205 | LC3 covalently coupled to agarose beads
23459205 | recombinant His-ubqln1
23459205 | recombinant His-ubqln4
23358411 | TUBE2-UBA

Hu, X. et al. (2012) "Rap80 Protein Recruitment to DNA Double-strand Breaks Requires Binding to Both Small Ubiquitin-like Modifier (SUMO) and Ubiquitin Conjugates." J. Biol. Chem. 287(30): 25510–25519.

22689573 | SUMO2 3– 8, agarose-SUMO2 beads, Ub Lys-63 2–7,

Durcan, T.M., et al. (2011) “The Machado–Joseph disease-associated mutant form of ataxin-3 regulates parkin ubiquitination and stability.” Human Molecular Genetics, 20: 141-154.

20940148 | His-ataxin-3 (14Q)

Gerlach, B., et al. (2011) "Linear ubiquitination prevents inflammation and regulates immune signalling.” Nature 471(7340): 591–596.

21455173 | agarose beads coupled to ubiquitin

LaLonde, D. and Bretscher, A. (2011) “The UBX Protein SAKS1 Negatively Regulates Endoplasmic Reticulum-associated Degradation and p97-dependent Degradation.” The Journal of Biological Chemistry, 286: 4892-4901.

21135095 | His6-tagged Ataxin-3

Sun, H., et al. (2011) “Bcr-Abl ubiquitination and Usp9x inhibition block kinase signaling and promote CML cell apoptosis.” Blood, 117: 3151-3162.

Bcr-Abl ubiquitination and Usp9x inhibition block kinase signaling and promote CML cell apoptosis. | ataxin-agarose beads, Rap80-agarose beads

Feng, L., et al. (2010) “The Lys63-specific Deubiquitinating Enzyme BRCC36 Is Regulated by Two Scaffold Proteins Localizing in Different Subcellular Compartments.” J. Biol. Chem. 285: 30982-30988.

20656690 | RAP80 UIM-agarose beads

Liang, J., et al. (2010) “MCP-induced protein 1 deubiquitinates TRAF proteins and negatively regulates JNK and NF-B signaling.” J. Exp. Med. 207: 2959-2973.

21115689 | ubiquitin-agarose

De Schutter, J., et al. (2009) “SHIP2 (SH2 Domain-containing Inositol Phosphatase 2) SH2 Domain Negatively Controls SHIP2 Monoubiquitination in Response to Epidermal Growth Factor.” J. Biol. Chem. 284: 36062 - 36076.

19880507 | ubiquitin-agarose beads

Lis, E. T. and F. E. Romesberg (2006). "Role of Doa1 in the Saccharomyces cerevisiae DNA Damage Response." Mol. Cell. Biol. 26(11): 4122-4133.

16705165 | ubiquitin agarose

Kowanetz K., et al. (2004) "Suppressors of T-cell Receptor Signaling Sts-1 and Sts-2 Bind to Cbl and Inhibit Endocytosis of Receptor Tyrosine Kinases." J. Biol. Chem. 279(31): 32786-32795.

15159412 | Ub-Agarose

Fractions

Zhang, S. et al. (2012) "GST:Identification of RNF8 as a ubiquitin ligase involved in targeting the p12 subunit of DNA polymerase δ for degradation in response to DNA damage." J. Biol. Chem.

23233665 | ATP regenerating solution (ENS), ubiquitin aldehyde, HeLa S100 fraction, Recombinant human E1 ubiquitin activating enzyme, Recombinant human ubiquitin, Recombinant human ubiquitin K48R, Recombinant human ubiquitin K63R, Recombinant human UbcH5c, Recombinant human Cdc34, Recombinant human UbcH2

Herr, R.A. et al. (2012) "Newly Discovered Viral E3 Ligase pK3 Induces Endoplasmic Reticulum-associated Degradation of Class I Major Histocompatibility Proteins and Their Membrane-bound Chaperones." J. Biol. Chem. 287(18): 14467–14479.

22403403 | Fraction II (FII) from rabbit reticulocyte extract

Watanabe, T. et al. (2012) "FBL2 regulates amyloid precursor protein (APP) metabolism by promoting ubiquitination-dependent APP degradation and inhibition of APP endocytosis." J. Neurosci. 32(10): 3352–3365.

Fraction II Reticulocyte Conjugation kit | 22399757

Kensche, T. et al. (2012) "Analysis of Nuclear Factor-κB (NF-κB) Essential Modulator (NEMO) Binding to Linear and Lysine-linked Ubiquitin Chains and Its Role in the Activation of NF-κB." J. Biol. Chem. 287(28): 23626–23634.

22605335 | creatine phosphokinase, HeLa-S-100 fraction II, ubiquitin, ubiquitin aldehyde

Herr, R.A. et al. (2012) "Newly Discovered Viral E3 Ligase pK3 Induces Endoplasmic Reticulum-associated Degradation of Class I Major Histocompatibility Proteins and Their Membrane-bound Chaperones." J. Biol. Chem. 287(18): 14467–14479.

22403403 | deubiquiting enzyme inhibitor ubiquitin aldehyde, proteasome inhibitor MG132

Arun, P., et al. (2011) “Senescence Marker Protein 30 (SMP30) Expression in Eukaryotic Cells: Existence of Multiple Species and Membrane Localization.” PLoS one, 6: e16545. doi:10.1371/journal.pone.0016545.

21347421 | S-100 Hela degradation mixture

Zhu, F., et al. (2011) “Phosphorylation of H2AX at Ser139 and a New Phosphorylation Site Ser16 by RSK2 Decreases H2AX Ubiquitination and Inhibits Cell Transformation.” Cancer Research, 71: 393.

21224359 | Fraction II (FII)-HeLa

Bhandari, D., et al. (2011) “Ubiquitination of mRNA cycling sequence binding protein from Leishmania donovani (LdCSBP) modulates the RNA endonuclease activity of its Smr domain.” FEBS Letters, 585: 809-813.

S-100 HeLa conjugation kit

Chaugule, V.K., et al. (2011) "Autoregulation of Parkin activity through its ubiquitin-like domain.” The EMBO Journal 30(14): 2853–2867.

21694720 | HeLa S100 fraction

Burnett, B.G., et al. (2009) "Regulation of SMN Protein Stability." Mol. Cell. Biol. 29: 1107-1115.

19103745 | ubiquitin conjugation enzyme fraction

Marteijn, J., et al. (2009) "The ubiquitin ligase Triad1 inhibits myelopoiesis through UbcH7 and Ubc13 interacting domains.” Leukemia 23(8): 1480–1489.

19340006 | reticulocyte fraction IIA

Wang, X., et al. (2009) “Ube2j2 ubiquitinates hydroxylated amino acids on ER-associated degradation substrates.” J. Cell Biol. 187: 655 - 668.

19951915 | FII from rabbit reticulocyte extract

Chiang M-H., et al. (2008) "Ubiquitin-Conjugating Enzyme UBE2D2 Is Responsible for FBXW2 (F-Box and WD Repeat Domain Containing 2)-Mediated Human GCM1 (Glial Cell Missing Homolog 1) Ubiquitination and Degradation." Biol Reprod 79(5):914-920.

18703417 | Recombinant CDC34

Liu, J., et al. (2008) "A therapeutic dose of doxorubicin activates ubiquitin-proteasome system-mediated proteolysis by acting on both the ubiquitination apparatus and proteasome." Am. J. Physiol. Heart.Circ. Physiol. 295(6): H2541-H2550.

18978187 | ubiquitin conjugation enzymes (F-340)

Todi, S. V., et al. (2007). "Cellular turnover of the polyglutamine disease protein ataxin-3 is regulated by its catalytic activity." J. Biol. Chem.: 282(40): 29348-29358.

17693639 | rabbit 26 S fractions

Zhu, F., et al. (2006). "COOH-Terminal Src Kinase-Mediated c-Jun Phosphorylation Promotes c-Jun Degradation and Inhibits Cell Transformation." Cancer Res. 66(11): 5729-5736.

16740711 | Fraction II HeLa Conjugation Kit

Doss-Pepe E. W., et al. (2005) "[alpha]-Synuclein and Parkin Contribute to the Assembly of Ubiquitin Lysine 63-linked Multiubiquitin Chains." J. Biol. Chem. 280(17): 16619-16624.

15718234 | Fraction II

Kaneko-Oshikawa C., et al. (2005) "Mammalian E4 Is Required for Cardiac Development and Maintenance of the Nervous System." Mol. Cell. Biol. 25(24): 10953-10964.

16314518 | S100 lysate protein

Marteijn J. A. F., et al. (2005) "The E3 ubiquitin-protein ligase Triad1 inhibits clonogenic growth of primary myeloid progenitor cells." Blood 106(13): 4114-4123.

16118314 | http://bloodjournal.hematologylibrary.org/content/106/13/4114.long

Song B.-L. and DeBose-Boyd R.A. (2004) "Ubiquitination of 3-Hydroxy-3-methylglutaryl-CoA Reductase in Permeabilized Cells Mediated by Cytosolic E1 and a Putative Membrane-bound Ubiquitin Ligase." J. Biol. Chem. 279(27): 28798-28806.

15090540 | Fraction I, Fraction II, S100 isolated from HeLa cells

Sampathu, D.M., et al. (2003) "Ubiquitination of [alpha]-synuclein is not required for formation of pathological inclusions in [alpha]--Synucleinopathies." Am. J. Path. 163: 91-100.

12819014 | ubiquitin conjugation fractions A and B (derived from mammalian cell lysate)

Shim, M., et al. (2003). "Lithium stabilizes the CCAAT/Enhancer-binding protein [alpha] (C/EBP[alpha]) through a glycogen synthase kinase 3 (GSK3)-independent pathway involving direct inhibitor of proteasomal activity." Genes Cells 11(7): 731-744.

12668682 | HeLa S-100 fraction

Ohh, M., et al. (2002). "An intact NEDD8 pathway is required for Cullin-dependent ubiquitylation in mammalian cells.” EMBO Reports 3(2): 177-182.

11818338 | HeLa FII