Material Data Sheet
ISG15 covalently coupled to agarose beads via primary amines allowing for a fully functional Cterminus. Useful for isolation and capture of ISG15 interacting proteins such as the ISG15 activating E1 enzyme, the ISG15 carrier enzyme Ubch8, ISG15 E3 ligases, UBP43 (USP18) and other proteins/enzymes that have an affinity for ISG15 protein.
Stock:0.5 ml of ISG15 agarose is supplied in 1 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl.
Use & Storage
Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.
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