UL-630

Material Data Sheet

ISG15 Agarose

Price: $159.00
Catalog #: UL-630

ISG15 Agarose

Price: 
$159
Quantity: 
500 µL
Data Sheet: 

ISG15 covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for isolation and capture of ISG15 interacting proteins such as the ISG15 activating E1 enzyme, the ISG15 carrier enzyme Ubch8, ISG15 E3 ligases, UBP43 (USP18) and other proteins/enzymes that have an affinity for ISG15 protein.

Product Information

Stock: 
0.5 ml of ISG15 agarose is supplied in 1 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl.

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

Storage: 

The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.

Literature

References: 

Dao C.T. and Zhang D-E (2005) Frontiers. Biosc. 10: 2710-2722
D’Cunha J. et al., (1996) Proc. Natl. Acad. Sci. 93:211-215
Jentsch S. and Pyrowolakis G. (2000) Tren. Cell. Biol. 10:335-342
Kim K.I. and Zhang D. (2003) Biochem. Biophys. Res. Comm. 307:431-434
Liakopoulos D., et al., (1998) EMBO J. 17:2208-2214
Muller S. et al., (2001) Nat. Rev. Mol. Cell. Biol. 2:202-210
Owhashi M. et al. (2003) Biochem. Biophys. Res. Comm. 309:533-539
Potter J.L. et al. (1999) J. Biol Chem. 267:25061068