Material Data Sheet

Di-Ubiquitin/Ub2 WT Chains (K33-linked) 

Price: $189.00
Catalog #: UC-101B

Di-Ubiquitin/Ub2 WT Chains (K33-linked) 

25 µg

Linkage specific poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB's") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules. Poly-Ubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF's and/or other Ubiquitin-sensing elements.

K33-linked Di-Ubiquitin chains are manufactured using recombinant Ubiquitin and purely enzymatic techniques to avoid the potential for contaminating synthetic intermediates. The correctness of linkage and purity of each production lot is assessed using the Absolute Quantitation of Ubiquitin method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information on the composition of poly-Ubiquitin samples.

Product Information

1 mg/ml (58 µM) in sterile, deionized water
17 kDa
>95% by SDS-PAGE, Ub-AQUA analysis provided for each lot

Store at –20°C.  Avoid multiple freeze/thaw cycles.