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We offer a range of methods to characterize your protein and can specifically modify proteins with a variety of labels. Depending on the specific properties of your protein, we can also perform activity and functional assays.
- Biophysical parameters (amino acid analysis, sequencing, circular dichroism, mass spectrometry, cysteine content determination)
- Solubility/stability screening with various storage conditions
- Soluble aggregate studies (analytical size exclusion chromatography)
- Enzyme activity
- Denaturing studies
- SDS-PAGE or Western blotting
- Modification using biochemical or enzymatic methods
- Avidin-biotin technology
- Antibody conjugation
- Fluorescent labeling
- Metabolic or biochemical labeling of non-radioactive labels
- Controlled proteolytic modification and deglycosylation
- Assessment of modification efficiencies, final product yield, purity, and mass
- Expertise in protein characterization and activity determination
- Enzymatic kinetic analysis using UV-Visible and fluorescence spectophotometric analysis (continuous or end point)
- Plate based assays
- HPLC based assays
- Gel based assays
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