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UBE1L2 Activating E1 Enzyme
(Uba6) is a newly identified E1 activating enzyme for ubiquitin. This enzyme charges a newly
identified E2 enzyme, Use1, which is not charged by UBE1 (Uba1). It also charges various E2 enzymes
including all UbcH5 isoforms, UbcH6, UbcH7, UbcH10 and UbcH13. Additionally, FAT10 forms a
thiolester complex with Use1 in the presence of UBE1L2 but not with UBE1. The UBE1L2 enzyme
does not activate other UBLs (including SUMO, NEDD8, ISG15, UFM1), indicating specificity for
ubiquitin and FAT10.
References: Jin J. et al., (2007) Nature 447:1135-1138. Pelzer C.,
et al., (2007) J. Biol. Chem. 282: 23010-23014.
Please note that higher resolution graphics are available in the PDF version of this document available at
Figure 1. Ubiquitin Thioester
Complex Formation with various E2 enzymes in the presence of the
Uba6 E1 enzyme. Reactions
containing GST-Uba6 (50 nM), E2 enzymes (5μM) and Ubiquitin
(100μM) were incubated in the
absence or presence of Mg-ATP (5mM) at 37 degrees C for 60
minutes. Proteins were separated by 16% SDS-PAGE
under non-reducing conditions. The § symbol denotes the Ub-E2
thiolester complexes.
Figure 2. FAT10:Use1 Thiolester
Complex Formation. Reactions
containing GST-Uba6 (50 nM),E2 enzymes
(5μM) and FAT10 (10μM) were incubated in the absence or presence
of Mg-ATP (5mM) at 37 degrees C for 60
minutes. Proteins were separated by 4-20% SDS-PAGE under
non-reducing conditions. Asterisks (*) denote the E2
enzyme proteins.
| Table 1: Thioester
Complex Formation Summary |
| UB:E2 Pair |
UBE1(Uba1) |
UBE1L2(Uba6) |
| Ub:Use1 |
X |
Yes |
| Ub:UbcH1 |
Yes |
X |
| Ub:UbcH2 |
Yes |
X |
| Ub:UbcH3 |
Yes |
X |
| Ub:UbcH5a |
Yes |
Yes |
| Ub:UbcH5b |
Yes |
Yes |
| Ub:UbcH5c |
Yes |
Yes |
| Ub:UbcH6 |
Yes |
Yes |
| Ub:UbcH7 |
Yes |
Yes |
| Ub:UbcH10 |
Yes |
Yes* |
| Ub:UbcH13 |
Yes |
Yes |
| Ub:Uev1a/UbcH13 |
Yes |
Yes |
| |
|
|
| UBL:E2 Pair |
UBE1(Uba1) |
UBE1L2(Uba6) |
| FAT10:Use1 |
X |
Yes |
| FAT10:UbcH1 |
X |
X |
| FAT10:UbcH3 |
X |
X |
| FAT10:UbcH5 |
X |
X |
| FAT10:UbcH6 |
X |
X |
| FAT10:UbcH7 |
X |
X |
| FAT10:UbcH13 |
X |
X |
| |
|
|
| NEDD8:UbcH12 |
X |
X |
| SUMO1:UbcH9 |
X |
X |
| SUMO2:UbcH9 |
X |
X |
| SUMO3:UbcH9 |
X |
X |
| ISG15:UbcH8 |
X |
X |
| UFM1:Ufc1 |
X |
X |
| * Weak thioester
formation, visible by Western blot only. |
|
Catalog Information |
| Activating Enzyme |
Catalog No. |
Size |
GST-Ubiquitin
Activating Enzyme, UBE1L2/UBA6
human recombinant |
|
25 μg |
| |
|
|
| E2 Congugating Enzyme |
Catalog No. |
Size |
His6-Use1,
human recombinant |
|
50 μg
100 μg |
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©1998-2005 Boston Biochem, Inc. All rights reserved. |
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