New E1 Activating Enzymes!

November 2003

SUMO-1/Smt3p Activating Enzyme (E1), Aos1p/Uba2p, S. cerevisiae
Cat.#: E-310 (GST-Tagged)
Cat.#: E-311

The Activating Enzyme, Aos1p/Uba2p dimer, catalyzes the activation of the C-terminal carboxyl group of SUMO-1 (human) and Smt3p (yeast) by forming a high-energy thioester bond in an ATP-dependent manner. The activated SUMO-1 or Smt3p is then transferred to a lysine of the target protein by UbcH9/UBC9. In humans, SUMO-1 is conjugated to a variety of proteins including RanGAP1, Ik Ba , and PML without the requirement of an E3 ligase. UbcH9-mediated IkBa modification by SUMO-1 inhibits the activation of NF-κB. SUMO-1 modification is implicated in the progression of G 2 to M phase cell cycle progression.


Nedd8 Activating Enzyme (E1), APPBP1/Uba3, human
Cat.#: E-312 (GST-Tagged)
Cat.#: E-313

The Nedd8 Activating Enzyme, APPBP1/Uba3 dimer, catalyzes the activation of the C-terminal carboxyl group of Nedd8 by forming a high-energy thioester bond in an ATP-dependent manner. The activated Nedd8 is then transferred to a lysine of the target protein by UbcH12 without the requirement of an E3 ligase. Nedd8 conjugation is implicated in the turnover regulation of p27(Kip1) and is known to modify Cdc53 and cullin family me mbers of ubiquitin-protein ligases.

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