Ubiquitin-Like Research Made Easy!

October 2004

ISG15 Products

The ubiquitin-like ISG15 is conjugated to intracellular target proteins in the presence of UbcH8 and the ISG15 E1 Activating Enzyme after IFN- a/b stimulation or microbial challenge. The ISGlyation pathway is distinct from ubiquitination and it appears that it does not target proteins for proteasomal degradation.

ISG15 Activating Enzyme (E1), human recombinant
Cat.#: E-308 (GST-tagged)
Cat.#: E-309 (untagged)

UbcH8, human recombinant
Cat.#: E2-643 (GST-tagged)
Cat.#: E2-644 (untagged)

ISG15 (UCRP), human recombinant
Cat.#: UL-600 (GST-tagged)
Cat.#: UL-601 (untagged)

SUMO Products

The ubiquitin-like SUMO-1 is conjugated to a variety of proteins in the presence of UbcH9 and the Aos1p/Uba2p (SAE1/SAE2 in human) Activating Enzyme. Sumolyation can occur without the requirement of a specific E3 ligase activity and substrates are primarily localized to the nucleus. SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation.

SUMO-1 Activating Enzyme (E1) (Aos1p/Uba2p), S. cerevisiae
Cat.#: E-310 (GST-tagged)
Cat.#: E-311 (untagged)

UbcH9, human recombinant
Cat.#: E2-645

SUMO-1 (Sentrin-1), human recombinant
Cat.#: UL-710 (GST-tagged)
Cat.#: UL-712 (untagged)

Anti-Sumo-1
Cat.#: A-712
Cat.#: A-645

Nedd8 Products

The ubiquitin-like protein Nedd8 is conjugated to targets by the Nedd8-specific E1 Activating Enzyme (App-Bp1/Uba3), the UbcH12 E2 enzyme, and the ROC1/Rbx1 RING finger E3 ligase. Nedd8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family.

Nedd8 Activating Enzyme (E1) (AppBp1/Uba3), human recombinant
Cat.#: E-312 (GST-tagged)
Cat.#: E-313 (untagged)

UbcH12, human recombinant
Cat.#: E2-655 (GST-tagged)
Cat.#: E2-656 (untagged)

Nedd8, human recombinant
Cat.#: UL-812 (untagged)
Cat.#: UL-813 (His-tagged)

Anti-Nedd8
Cat.#: A-812

Anti-UbcH12
Cat.#: A-655