New Enzymes for your Ubiquitin-Related Research!

June 2004

Ubiquitin Activating Enzyme (E1), human
Cat.#: E-305

GST-Ubiquitin Activating Enzyme (E1), human
Cat.#: E-306

Enzyme responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. E1 is a critical component for the initiation of any in vitro conjugation reactions.

UbcH5c
Cat.#: E2-627

UbcH5s are human homologs of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. UbcH5 mediates the degradation of a myriad of short-lived regulatory proteins including the tumor suppressor p53 in the presence of E6/E6-AP or MDM2, the c-Fos transcription factor, the signal-induced ubiquitination of Ik Ba, as well as the processing of precursor of NF-κB1, p105.